메뉴 건너뛰기




Volumn 200, Issue 1-2, 1997, Pages 69-77

High level production of soluble single chain antibodies in small-scale Escherichia coli cultures

Author keywords

bacterial expression system; single chain Fv antibody fragment; small scale culture

Indexed keywords

CD3 ANTIGEN;

EID: 0031022084     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(96)00188-3     Document Type: Article
Times cited : (167)

References (36)
  • 3
    • 0025123399 scopus 로고
    • Folding and aggregation of β-lactamase in the periplasmic space of escherichia coli
    • Bowden, G.A. and Georgiou, G. (1990) Folding and aggregation of β-lactamase in the periplasmic space of Escherichia coli. J. Biol. Chem. 265, 16760.
    • (1990) J. Biol. Chem. , vol.265 , pp. 16760
    • Bowden, G.A.1    Georgiou, G.2
  • 4
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248.
    • (1976) Anal. Biochem. , vol.72 , pp. 248
    • Bradford, M.M.1
  • 6
    • 0006454079 scopus 로고
    • Renaturation, purification and characterization of recombinant fab-fragments produced in escherichia coli
    • Buchner, J. and Rudolph, R. (1991) Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli. Bio/Technology 9, 157.
    • (1991) Bio/technology , vol.9 , pp. 157
    • Buchner, J.1    Rudolph, R.2
  • 7
    • 0026792807 scopus 로고
    • A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies
    • Buchner, J., Pastan, I. and Brinkmann, U. (1992) A method for increasing the yield of properly folded recombinant fusion proteins: single-chain immunotoxins from renaturation of bacterial inclusion bodies. Anal. Biochem. 205, 263.
    • (1992) Anal. Biochem. , vol.205 , pp. 263
    • Buchner, J.1    Pastan, I.2    Brinkmann, U.3
  • 8
    • 0027975979 scopus 로고
    • Intra- and extracellular expression of an scfv antibody fragment in e. Coli: Effect of bacterial strains and pathway engineering using groes/l chaperonins
    • Duenas, M., Vazquez, J., Ayala, M., Söderlind, E., Ohlin, M., Perez, L., Borrebaeck, C.A.K. and Gavilondo, J.V. (1994) Intra- and extracellular expression of an scFv antibody fragment in E. coli: effect of bacterial strains and pathway engineering using GroES/L chaperonins. BioTechniques 16, 476.
    • (1994) Biotechniques , vol.16 , pp. 476
    • Duenas, M.1    Vazquez, J.2    Ayala, M.3    Söderlind, E.4    Ohlin, M.5    Perez, L.6    Borrebaeck, C.A.K.7    Gavilondo, J.V.8
  • 10
    • 0024448151 scopus 로고
    • Calculation of protein extinction coefficients from amino acid sequence data
    • Gill, S.C. and von Hippel, P.H. (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182, 319.
    • (1989) Anal. Biochem. , vol.182 , pp. 319
    • Gill, S.C.1    Von Hippel, P.H.2
  • 11
    • 0025162270 scopus 로고
    • A comparison of strategies to stabilize immunoglobulin fv-fragments
    • Glockshuber, R., Malia, M., Pfitzinger, I. and Plückthun, A. (1990) A comparison of strategies to stabilize immunoglobulin Fv-fragments. Biochemistry 29, 1362.
    • (1990) Biochemistry , vol.29 , pp. 1362
    • Glockshuber, R.1    Malia, M.2    Pfitzinger, I.3    Plückthun, A.4
  • 14
    • 14744271625 scopus 로고
    • Protein aggregation in vitro and in vivo: A quantitative model of the kinetic competition between folding and aggregation
    • Kiefhaber, T., Rudolf, R., Kohler, H.-H. and Buchner, J. (1991) Protein aggregation in vitro and in vivo: a quantitative model of the kinetic competition between folding and aggregation. Bio/Technology 9, 825.
    • (1991) Bio/technology , vol.9 , pp. 825
    • Kiefhaber, T.1    Rudolf, R.2    Kohler, H.-H.3    Buchner, J.4
  • 15
    • 0028001154 scopus 로고
    • Recombinant single-chain fv fragments carrying c-terminal cysteine residues: Production of bivalent and biotinylated miniantibodies
    • Kipriyanov, S.M., Dübel, S., Breitling, F., Kontermann, R.E. and Little, M. (1994) Recombinant single-chain Fv fragments carrying C-terminal cysteine residues: production of bivalent and biotinylated miniantibodies. Mol. Immunol. 31, 1047.
    • (1994) Mol. Immunol. , vol.31 , pp. 1047
    • Kipriyanov, S.M.1    Dübel, S.2    Breitling, F.3    Kontermann, R.E.4    Little, M.5
  • 16
    • 0029877588 scopus 로고    scopus 로고
    • Affinity enhancement of a recombinant antibody: Formation of complexes with multiple valency by a single-chain fv fragment-core streptavidin fusion
    • Kipriyanov, S.M., Little, M., Kropshofer, H., Breitling, F., Gotter, S. and Dübel, S. (1996a) Affinity enhancement of a recombinant antibody: formation of complexes with multiple valency by a single-chain Fv fragment-core streptavidin fusion. Protein Eng. 9, 203.
    • (1996) Protein Eng. , vol.9 , pp. 203
    • Kipriyanov, S.M.1    Little, M.2    Kropshofer, H.3    Breitling, F.4    Gotter, S.5    Dübel, S.6
  • 17
    • 0029793209 scopus 로고    scopus 로고
    • Rapid detection of recombinant antibody fragments directed against cell-surface antigens by flow cytometry
    • Kipriyanov, S.M., Kupriyanova, O.A., Little, M. and Moldenhauer, G. (1996b) Rapid detection of recombinant antibody fragments directed against cell-surface antigens by flow cytometry. J. Immunol. Methods 196, 51.
    • (1996) J. Immunol. Methods , vol.196 , pp. 51
    • Kipriyanov, S.M.1    Kupriyanova, O.A.2    Little, M.3    Moldenhauer, G.4
  • 18
    • 0027526906 scopus 로고
    • The effect of folding catalysts on the in vivo folding process of different antibody fragments expressed in escherichia coli
    • Knappik, A., Krebber, C. and Plückthun, A. (1993) The effect of folding catalysts on the in vivo folding process of different antibody fragments expressed in Escherichia coli. Bio/Technology 11, 77.
    • (1993) Bio/technology , vol.11 , pp. 77
    • Knappik, A.1    Krebber, C.2    Plückthun, A.3
  • 19
    • 0028966985 scopus 로고
    • Engineered turns of a recombinant antibody improve its in vivo folding
    • Knappik, A. and Plückthun, A. (1995) Engineered turns of a recombinant antibody improve its in vivo folding. Protein Eng. 8, 81.
    • (1995) Protein Eng. , vol.8 , pp. 81
    • Knappik, A.1    Plückthun, A.2
  • 20
    • 0023613953 scopus 로고
    • Rapid and efficient site-specific mutagenesis without phenotypic selection
    • Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154, 367.
    • (1987) Methods Enzymol. , vol.154 , pp. 367
    • Kunkel, T.A.1    Roberts, J.D.2    Zakour, R.A.3
  • 21
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of the bacteriophage t4
    • Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227, 680.
    • (1970) Nature , vol.227 , pp. 680
    • Laemmli, U.K.1
  • 22
    • 0345077411 scopus 로고
    • Promoters largely determine the efficiency of repressor action
    • Lanzer, M. and Bujard, H. (1988) Promoters largely determine the efficiency of repressor action. Proc. Natl. Acad. Sci. USA 85, 8973.
    • (1988) Proc. Natl. Acad. Sci. USA , vol.85 , pp. 8973
    • Lanzer, M.1    Bujard, H.2
  • 23
    • 0028285385 scopus 로고
    • Recombinant single-chain antibody peptide conjugates expressed in escherichia coli for the rapid diagnosis of hiv
    • Lilley, G.G., Dolezal, O., Hillyard, C.J., Bernard, C. and Hudson, P. (1994) Recombinant single-chain antibody peptide conjugates expressed in Escherichia coli for the rapid diagnosis of HIV. J. Immunol. Methods 171, 211.
    • (1994) J. Immunol. Methods , vol.171 , pp. 211
    • Lilley, G.G.1    Dolezal, O.2    Hillyard, C.J.3    Bernard, C.4    Hudson, P.5
  • 26
    • 0026327378 scopus 로고
    • Construction, binding properties, metabolism, and tumor targeting of a single-chain fv derived from the pancarcinoma monoclonal antibody cc49
    • Milenic, D.E., Yokota, T., Filpula, D.R., Finkelman, M.A.J., Dodd, S.W., Wood, J.F., Whitlow, M., Snoy P. and Schlom, J. (1991) Construction, binding properties, metabolism, and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49. Cancer Res. 51, 6363.
    • (1991) Cancer Res. , vol.51 , pp. 6363
    • Milenic, D.E.1    Yokota, T.2    Filpula, D.R.3    Finkelman, M.A.J.4    Dodd, S.W.5    Wood, J.F.6    Whitlow, M.7    Snoy, P.8    Schlom, J.9
  • 28
    • 0027450561 scopus 로고
    • The complete general secretory pathway in gram-negative bacteria
    • Pugsley, A.P. (1993) The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57, 50.
    • (1993) Microbiol. Rev. , vol.57 , pp. 50
    • Pugsley, A.P.1
  • 30
    • 0026350603 scopus 로고
    • Secretion and in vivo folding of the fab fragment of the antibody mcpc603 in escherichia coli: Influence of disulfides and cis-prolines
    • Skerra, A. and Plückthim, A. (1991) Secretion and in vivo folding of the Fab fragment of the antibody McPC603 in Escherichia coli: influence of disulfides and cis-prolines. Protein Eng. 4, 971.
    • (1991) Protein Eng. , vol.4 , pp. 971
    • Skerra, A.1    Plückthim, A.2
  • 31
    • 0027312336 scopus 로고
    • Bacterial expression of immunoglobulin fragments
    • Skerra, A. (1993) Bacterial expression of immunoglobulin fragments. Curr. Opin. Immunol. 5, 256.
    • (1993) Curr. Opin. Immunol. , vol.5 , pp. 256
    • Skerra, A.1
  • 33
    • 0018836897 scopus 로고
    • Okt3: A monoclonal anti-human t lymphocyte antibody with potent mytogenic properties
    • Van Wauwe, J.P, DeMey, J.R. and Goossens, J.G. (1980) OKT3: a monoclonal anti-human T lymphocyte antibody with potent mytogenic properties. J. Immunol. 124, 2708.
    • (1980) J. Immunol. , vol.124 , pp. 2708
    • Van Wauwe, J.P.1    Demey, J.R.2    Goossens, J.G.3
  • 36
    • 0026684815 scopus 로고
    • Rapid tumor penetration of a single-chain fv and comparison with other immunoglobulin forms
    • Yokota, T., Milenic, D.E., Whitlow, M. and Schlom, J. (1992) Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms. Cancer Res. 52, 3402.
    • (1992) Cancer Res. , vol.52 , pp. 3402
    • Yokota, T.1    Milenic, D.E.2    Whitlow, M.3    Schlom, J.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.