High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor

Microbial Cell Factories

Volumn 9, Issue , 2010, Pages

  Glazyrina, Julia ^a   Materne, Eva Maria ^a   Dreher, Thomas ^b   Storm, Dirk ^a   Junne, Stefan ^a   Adams, Thorsten ^b   Greller, Gerhard ^b   Neubauer, Peter ^a  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^b SARTORIUS STEDIM BIOTECH GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DEHYDROGENASE; GLUCOSE; OXYGEN; RECOMBINANT PROTEIN;

AEROBIC CELL CULTURE; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; BATCH PROCESS; BIOREACTOR; CELL DENSITY; COMPUTER SYSTEM; CONTROL SYSTEM; CONTROLLED STUDY; DRY WEIGHT; EQUIPMENT DESIGN; ESCHERICHIA COLI; FED BATCH CULTURE; HETEROLOGOUS EXPRESSION; NONHUMAN; NUTRIENT SUPPLY; OPTICAL DENSITY; PROTEIN SYNTHESIS; BIOSYNTHESIS; CULTURE TECHNIQUE; ENZYMOLOGY; EVALUATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; INSTRUMENTATION; MICROBIOLOGY;

ESCHERICHIA COLI;

ALCOHOL DEHYDROGENASE; BIOREACTORS; CELL CULTURE TECHNIQUES; ESCHERICHIA COLI; RECOMBINANT PROTEINS;

EID: 77952680059     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-9-42     Document Type: Article

References (26)

1. Eibl R, Kaiser S, Lombriser R, Eibl D. Disposable bioreactors: the current state-of-the-art and recommended applications in biotechnology. Appl Microbiol Biotechnol 2010, 86:41-49. 10.1007/s00253-009-2422-9, 20094714.
2. Eibl R, Eibl D. Application of Disposable Bag-Bioreactors in Tissue Engineering and for the Production of Therapeutic Agents. Adv Biochem Eng Biotechnol 2009, 112:183-207.
3. Eibl R, Werner S, Eibl D. Disposable bioreactors for plant liquid cultures at Litre-scale. Engineering in Life Sciences 2009, 9:156-164.
4. Eibl R, Werner S, Eibl D. Bag Bioreactor Based on Wave-Induced Motion: Characteristics and Applications. Adv Biochem Eng Biotechnol 2010, 115:55-87.
5. Hanson MA, Brorson KA, Moreira AR, Rao G. Comparisons of optically monitored small-scale stirred tank vessels to optically controlled disposable bag bioreactors. Microb Cell Fact 2009, 8:44. 10.1186/1475-2859-8-44, 2731076, 19656387.
6. Singh V. Disposable bioreactor for cell culture using wave-induced agitation. Cytotechnology 1999, 30:149-158. 10.1023/A:1008025016272, 19003364.
7. Weber W, Weber E, Geisse S, Memmert K. Optimisation of protein expression and establishment of the Wave Bioreactor for Baculovirus/insect cell culture. Cytotechnology 2002, 38:77-85. 10.1023/A:1021102015070, 19003089.
8. Ling WLW, Deng L, Lepore J, Cutler C, Connon-Carlson S, Wang Y, Voloch M. Improvement of monoclonal antibody production in hybridoma cells by dimethyl sulfoxide. Biotechnology Progress 2003, 19:158-162. 10.1021/bp020068d, 12573019.
9. Ekström D, Cheng W, Andersson R, Mitra G, Zhu JW. Adenovirus production and recovery using a wave bioreactor. Abstracts of Papers of the American Chemical Society 2004, 227:U202.
10. Haldankar R, Li DQ, Saremi Z, Baikalov C, Deshpande R. Serum-free suspension large-scale transient transfection of CHO cells in WAVE bioreactors. Molecular Biotechnology 2006, 34:191-199. 10.1385/MB:34:2:191, 17172664.
11. Slivac I, Srcek VG, Radosevic K, Kniewald Z. Application of wave bioreactor for the production of BHK 21 and CHO cells biomass. Toxicology Letters 2005, 158:S217.
12. Chen HF, Zhou SZ, Pierce GP, Colosi P. Adaptation of the wave bioreactor to baculoviral production of AAV vectors: Scale-up considerations. Molecular Therapy 2004, 9:S160.
13. Palazon J, Mallol A, Eibl R, Lettenbauer C, Cusido RM, Pinol MT. Growth and ginsenoside production in hairy root cultures of Panax ginseng using a novel bioreactor. Planta Medica 2003, 69:344-349. 10.1055/s-2003-38873, 12709902.
14. Ritala A, Wahlstrom EH, Holkeri H, Hafren A, Makelainen K, Baez J, Makinen K, Nuutila AM. Production of a recombinant industrial protein using barley cell cultures. Protein Expression and Purification 2008, 59:274-281. 10.1016/j.pep.2008.02.013, 18406168.
15. Kilani J, Lebeault JM. Study of the oxygen transfer in a disposable flexible bioreactor with surface aeration in vibrated medium. Applied Microbiology and Biotechnology 2007, 74:324-330. 10.1007/s00253-006-0682-1, 17136538.
16. Mikola M, Seto J, Amanullah A. Evaluation of a novel Wave Bioreactor cellbag for aerobic yeast cultivation. Bioprocess Biosyst Eng 2007, 30:231-241. 10.1007/s00449-007-0119-y, 17340094.
17. Ullah M, Burns T, Bhalla A, Beltz HW, Greller G, Adams T. Disposable Bioreactors for Cells and Microbes Productivities similar to those achieved with stirred tanks can be achieved with disposable bioreactors. Biopharm International 2008, 44.
18. Krause M, Ukkonen K, Haataja T, Ruottinen M, Glumoff T, Neubauer A, Neubauer P, Vasala A. A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures. Microb Cell Fact 2010, 9:11. 10.1186/1475-2859-9-11, 2841585, 20167131.
19. Panula-Perälä J, Siurkus J, Vasala A, Wilmanowski R, Casteleijn MG, Neubauer P. Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks. Microb Cell Fact 2008, 7:31. 10.1186/1475-2859-7-31, 2588551, 19017379.
20. Soini J, Ukkonen K, Neubauer P. High cell density media for Escherichia coli are generally designed for aerobic cultivations - consequences for large-scale bioprocesses and shake flask cultures. Microb Cell Fact 2008, 7:26. 10.1186/1475-2859-7-26, 2526063, 18687130.
21. Viitanen MI, Vasala A, Neubauer P, Alatossava T. Cheese whey-induced high-cell-density production of recombinant proteins in Escherichia coli. Microb Cell Fact 2003, 2:2. 10.1186/1475-2859-2-2, 155635, 12740045.
22. Riesenberg D, Schulz V, Knorre WA, Pohl HD, Korz D, Sanders EA, Ross A, Deckwer WD. High cell density cultivation of Escherichia coli at controlled specific growth rate. J Biotechnol 1991, 20:17-27. 10.1016/0168-1656(91)90032-Q, 1367313.
23. Reuss M. Oxygen transfer and mixing: scale-up implications. 2nd completely revised edition 2009, 185-217. Weinheim: Wiley-VCH, Reed G, Stephanopoulos G.
24. Neubauer P, Haggström L, Enfors SO. Influence of substrate oscillations on acetate formation and growth yield in Escherichia coli glucose limited fed-batch cultivations. Biotechnol Bioeng 1995, 47:139-146. 10.1002/bit.260470204, 18623386.
25. Nancib N, Branlant C, Boudrant J. Metabolic roles of peptone and yeast extract for the culture of a recombinant strain of Escherichia coli. J Ind Microbiol 1991, 8:165-169. 10.1007/BF01575849, 1367899.
26. Siurkus J, Panula-Perälä J, Horn U, Kraft M, Rimseliene R, Neubauer P. Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures. Microb Cell Fact 2010, 9:35. 10.1186/1475-2859-9-35, 20487563.