Optimizing the expression of a monoclonal antibody fragment under the transcriptional control of the escherichia coli lac promoter

Canadian Journal of Microbiology

Volumn 46, Issue 6, 2000, Pages 532-541

  Donovan, Robert S ^a   Robinson, Campbell W ^a   Glick, Bernard R ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; GLUCOSE; GLYCEROL; IMMUNOGLOBULIN F(AB) FRAGMENT; ISOPROPYL THIOGALACTOSIDE; LACTOSE; MONOCLONAL ANTIBODY;

ANTIBODY PRODUCTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN INDUCTION; TRANSCRIPTION REGULATION;

ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA;

EID: 0033922059     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/cjm-46-6-532     Document Type: Article

References (35)

1. Barbas, C.F., Kang, A.S., Lerner, R.A., and Benkovic, S.J. 1991. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc. Natl. Acad. Sci. U.S.A. 88: 7978-7982.
2. Beckwith, J.R. 1987. The Lactose Operon. In Escherichia coli and Salmonella typhimurium. Vol. 2. American Society for Microbiology. Washington, D.C. pp. 1444-1452.
3. Bentley, W.E., Mirjalili, N., Anderson, D.C., Davis, R.H., and Kompala, D.S. 1990. Plasmid encoded protein: the principal factor of "metabolic burden" associated with recombinant bacteria. Biotechnol. Bioeng. 35: 668-681.
4. Bentley, W.E., Davis, R.H., and Kompala, D.S. 1991. Dynamics of CAT expression in E. coli. Biotechnol. Bioeng. 38: 749-760.
5. BioRad Laboratories 1993. Mini-Protean II dual slab cell instruction manual. BioRad Laboratories Inc., Richmond, Calif.
6. BioRad Laboratories 1994. Mini-Trans blot instruction manual. BioRad Laboratories Inc., Richmond, Calif.
7. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72: 248-254.
8. Brent, R., and Ptashne, M. 1981. Mechanism of action of lexA gene product. Proc. Natl. Acad. Sci. U.S.A. 78: 4204-4208.
9. Carrier, J.E., Nugent, M.E., Tacon, W.C.A., and Primrose, S.B. 1983. High expression of cloned genes in E. coli and its consequences. Trends Biotechnol. 1: 109-113.
10. Carter, P., Kelley, R.F., Rodrigues, M.L., Snedecor, B., Covarrubias, M., Velligan, M., Wong, W.L., Rowland, A.M., Kotts, C.E., Carver, M.E., Yang, M., Bourell, J.H., Shepard, H.M., and Henner, D. 1992. High level Escherichia coli expression and production of a bivalent humanized antibody fragment. Bio/Technology, 10: 163-167.
11. DeBellis, D., and Schwartz, I. 1990. Regulated expression of foreign genes fused to lac: control by glucose levels in the growth medium. Nucleic Acids Res. 18: 1311-1317.
12. Donovan, R.S., Robinson, C.W., and Glick, B.R. 1994. Detection enhancement of computer images of bands from western blots using a greyscale scanner. Biotechniques, 17: 660-661.
13. Donovan, R.S., Robinson, C.W., and Glick, B.R. 1995. An inexpensive system to provide sparged aeration to shake flask cultures. Biotechnol. Techniques, 9: 665-670.
14. Georgiou, G., and Shuler, M.L. 1988. Release of periplasmic enzymes and other physiological effects of β-lactamase overproduction in Escherichia coli. Biotechnol. Bioeng. 32: 741-748.
15. Glick, B.R. 1995. Metabolic load and heterologous gene expression. Biotechnol. Adv. 13: 247-261.
16. Glick, B.R., and Pasternak, J.J. 1998. Molecular Biotechnology: Principles and applications of recombinant DNA. 2nd ed. American Society for Microbiology, Washington, D.C.
17. Glick, B.R., and Whitney, G.K. 1987. Factors affecting the expression of foreign proteins in Escherichia coli. J. Indust. Microbiol. 1: 277-282.
18. Itakura, K., Hirose, T., Crea, R., and Riggs, A.D. 1977. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science, 198: 1056-1063.
19. Khosla, C., Curtis, J.E., Bydalek, P. Schwartz, J.R., and Bailey, J.E. 1990. Expression of recombinant proteins in Escherichia coli using an oxygen-responsive promoter. Bio/Technology, 8: 554-558.
20. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
21. Miller, J.H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratories. Cold Spring Harbor, N.Y.
22. Neubauer, P., Hofmann, K., Holst, O., Mattiason, B., and Kruschke, P. 1992. Maximizing the expression of a recombinant gene in Escherichia coli by manipulation of induction time using lactose as inducer. Appl. Microbiol. Biotechnol. 36: 739-744.
23. Oxer, M.D., Bently, C.M., Doyle, J.G., Peakman, T.C., Charles, I.G., and Makoff, A. 1991. High level heterologous expression in E. coli using the anaerobically-activated nirB promoter. Nucleic Acids Res. 19: 1899-1892.
24. Postma, P.W., Broekhuizen, C.P., and Geerse, R.H. 1989. The role of the PEP:carboyhdrate phosphotransferase system in the regulation of bacterial metabolism. FEMS Microbiol. Rev. 63: 69-80.
25. Raibound, E., Stanssens, P., and Fiers, F.W. 1981. Plasmid vectors for high efficiency expression controlled by the pL promoter of coliphage lambda. Gene, 15: 81-93.
26. Reznikoff, W.S., and Abelson, J.N. 1980. The lac promoter. In The operon. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y. pp. 221-224.
27. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
28. Shibui, T., and Nagahari, K. 1992. Secretion of a functioinal Fab fragment in Escherichia coli and the influence of culture conditions. Appl. Microbiol. Biotechnol. 37: 352-357.
29. Skerra, A., and Pluckthun, A. 1991. Secretion and in vivo folding of the Fab fragment of the antibody McPC603 in Escherichia coli: influence of disulphides and cis-prolines. Prot. Eng. 4: 971-979.
30. Skerra, A., Pfitzinger, I., and Pluckthun, A. 1991. The functional expression of antibody Fv fragments in Escherichia coli: improved vectors and a generally applicable purification technique. Bio/Technology, 9: 273-278.
31. Straight, J.V., and Ramkrishna, D. 1989. Bacterial growth on lactose: an experimental investigation. Biotechnol. Bioeng. 34: 705-716.
32. Towbin, H., Staehlin, T., and Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354.
33. Whitney, G.K., Glick, B.R., and Robinson, C.W. 1989. Induction of T4 DNA ligase in a recombinant strain of Escherichia coli. Biotechnol. Bioeng. 33: 991-998.
34. Wood, T.K., and Peretti, S.W. 1991. Effect of chemically-induced, cloned-gene expression on protein synthesis in E. coli. Biotechnol. Bioeng. 38: 397-412.
35. Yabuta, M., Onai-Miura, S., and Ohsuye, K. 1995. Thermoinducible expression of a recombinant fusion protein by Escherichia coli lac repressor mutants. J. Biotechnol. 39: 67-73.