Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support

Protein Expression and Purification

Volumn 52, Issue 1, 2007, Pages 194-201

  Hu, Xuejun ^a   O'Hara, Liam ^b   White, Simon ^a   Magner, Edmond ^a   Kane, Marian ^b   Gerard Wall, J ^a  

^a UNIVERSITY OF LIMERICK   (Ireland)

^b NATIONAL UNIVERSITY OF IRELAND   (Ireland)

Author keywords

Chaperone; Domoic acid; Escherichia coli; Immunosensor; Mesoporous silicate; Recombinant antibody; scFv

Indexed keywords

CHAPERONE; DOMOIC ACID; DRUG DERIVATIVE; IMMUNOGLOBULIN FRAGMENT; KAINIC ACID; NEUROTOXIN; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETICS; GENOTYPE; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; METABOLISM; MOLECULAR CLONING; RESTRICTION MAPPING;

CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENOTYPE; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; KAINIC ACID; MOLECULAR CHAPERONES; NEUROTOXINS; RECOMBINANT PROTEINS; RESTRICTION MAPPING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33845957062     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.08.009     Document Type: Article

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