Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli

Journal of Molecular Biology

Volumn 320, Issue 1, 2002, Pages 1-10

  Jurado, Paola ^a   Ritz, Daniel ^b   Beckwith, Jon ^b   De Lorenzo, Víctor ^a   Fernández, Luis A ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Disulfide bond; E. coli; Intrabodies; Recombinant antibodies; Single chain Fv

Indexed keywords

CHAPERONE; IMMUNOGLOBULIN; ISOMERASE; PROTEIN DISULFIDE REDUCTASE (GLUTATHIONE); SIGMA FACTOR; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; THIOREDOXIN REDUCTASE;

ANTIBODY PRODUCTION; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; CYTOPLASM; DISULFIDE BOND; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE MUTATION; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; REDUCTION;

ESCHERICHIA COLI;

EID: 0036296338     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00405-9     Document Type: Article

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