Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

Microbial Cell Factories

Volumn 9, Issue , 2010, Pages

  Šiurkus, Juozas ^b   Panula Perälä, Johanna ^c   Horn, Uwe ^d   Kraft, Mario ^d   Rimšeliene, Renata ^b   Neubauer, Peter ^a,c  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^b Fermentas UAB   (Lithuania)

^c UNIVERSITY OF OULU   (Finland)

^d HANS KNÖLL INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MINERAL; RIBONUCLEASE INHIBITOR; SODIUM CHLORIDE;

ARTICLE; BACTERIUM CULTURE; BINDING SITE; BIOPROCESS; BIOREACTOR; CELL DENSITY; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE OPTIMIZATION; ESCHERICHIA COLI; EXPRESSION VECTOR; FED BATCH CULTURE; GENE FUSION; GENE TECHNOLOGY; GROWTH RATE; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN TARGETING; RIBOSOME; SCALE UP; CELL CLONE; CYTOLOGY; METHODOLOGY; MICROARRAY ANALYSIS; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PILOT STUDY;

BACTERIOLOGICAL TECHNIQUES; BIOREACTORS; CLONE CELLS; CULTURE MEDIA; ESCHERICHIA COLI; INDUSTRIAL MICROBIOLOGY; MICROARRAY ANALYSIS; PILOT PROJECTS;

EID: 77952397828     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-9-35     Document Type: Article

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