High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

Microbial Cell Factories

Volumn 10, Issue , 2011, Pages

  Ukkonen, Kaisa ^a,b   Vasala, Antti ^a   Ojamo, Heikki ^b   Neubauer, Peter ^c  

^a BioSilta Oy   (Finland)

^b UNIVERSITY OF OULU   (Finland)

^c TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

Author keywords

Fed batch; Oxygen transfer; Recombinant protein; Shake flask

Indexed keywords

ALCOHOL DEHYDROGENASE; GLUCOSE; POLYSACCHARIDE; RECOMBINANT PROTEIN; BACTERIAL PROTEIN; OXYGEN;

ARTICLE; CELL DENSITY; CULTURE MEDIUM; DEVICES; ESCHERICHIA COLI; FED BATCH CULTURE; NONHUMAN; OXYGEN TRANSPORT; PROTEIN EXPRESSION; QUANTUM YIELD; TECHNOLOGY; BATCH CELL CULTURE; ENZYMOLOGY; EVALUATION; FERMENTATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; INSTRUMENTATION; LACTOBACILLUS; METABOLISM; METHODOLOGY;

ALCOHOL DEHYDROGENASE; BACTERIAL PROTEINS; BATCH CELL CULTURE TECHNIQUES; CULTURE MEDIA; ESCHERICHIA COLI; FERMENTATION; GLUCOSE; LACTOBACILLUS; OXYGEN; RECOMBINANT PROTEINS;

EID: 83155185459     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-10-107     Document Type: Article

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