High-level expression and characterization of an anti-VEGF165 single-chain variable fragment (scFv) by small ubiquitin-related modifier fusion in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 81, Issue 2, 2008, Pages 311-317

  Ye, Tingmei ^a   Lin, Zhihua ^a   Lei, Huanzong ^a  

^a LISHUI UNIVERSITY   (China)

Author keywords

Purification; scFv; Small ubiquitin related modifier; Vascular endothelial growth factor

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINES; AMINO ACIDS; ANTIBODIES; CELL PROLIFERATION; CHROMATOGRAPHY; DESORPTION; ENDOTHELIAL CELLS; ESCHERICHIA COLI; FUSION REACTIONS; HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NICKEL ALLOYS; ORGANIC ACIDS; PROTEINS; PURIFICATION; SELF ASSEMBLY;

AMINO ACID SEQUENCING; ANTIBODY FRAGMENTS; CELLULAR PROTEINS; ENDOTHELIAL CELL PROLIFERATIONS; EXPRESSION LEVELS; FUSION GENES; FUSION PROTEINS; HIGH YIELDS; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIES; HUMAN THERAPEUTICS; HUMAN UMBILICAL VEINS; IN VITRO ACTIVITIES; INTRAMOLECULAR DISULFIDE BONDS; LASER DESORPTION/IONIZATION; MASS SPECTROSCOPIES; NATIVE SEQUENCES; NITRILOTRIACETIC ACIDS; PRIMARY STRUCTURES; RECOMBINANT ANTIBODIES; SCFV; SMALL UBIQUITIN-RELATED MODIFIER; TIME OF FLIGHTS; UBIQUITIN; VARIABLE FRAGMENTS; VASCULAR ENDOTHELIAL GROWTH FACTOR; YIELD PRODUCTIONS;

CHROMATOGRAPHIC ANALYSIS;

HYBRID PROTEIN; NITRILOTRIACETIC ACID; RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; SUMO PROTEIN; VASCULOTROPIN 165; VASCULOTROPIN ANTIBODY;

ANTIBODY; BACTERIUM; BIOASSAY; GENE EXPRESSION; PROTEIN; RECOMBINATION;

AMINO TERMINAL SEQUENCE; ANTIBODY AFFINITY; ARTICLE; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISULFIDE BOND; ENDOTHELIUM CELL; ESCHERICHIA COLI; GENE CONSTRUCT; GENE EXPRESSION; GENE FUSION; HUMAN; HUMAN CELL; NONHUMAN; PLASMID; PROTEIN PURIFICATION; UMBILICAL VEIN;

ANTIBODIES; CELL PROLIFERATION; CELLS, CULTURED; CHROMATOGRAPHY, AFFINITY; ENDOTHELIAL CELLS; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUMO-1 PROTEIN; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 55649112043     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1655-3     Document Type: Article

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