A fed-batch based cultivation mode in Escherichia coli results in improved specific activity of a novel chimeric-truncated form of tissue plasminogen activator

Journal of Applied Microbiology

Volumn 114, Issue 2, 2013, Pages 364-372

  Mahboudi, F ^a   Barkhordari, F ^a   Godarzi, R M ^a   Enayati, S ^a   Davami, F ^a  

^a PASTEUR INSTITUTE OF IRAN   (Iran)

Author keywords

Escherichia coli expression; Fed batch cultivation; Specific activity; Tissue type plasminogen activator

Indexed keywords

BATCH DATA PROCESSING; PURIFICATION; RECOMBINANT PROTEINS; TISSUE;

CULTIVATION MODES; E. COLI; ESCHERICHIA COLI EXPRESSION; FED BATCHES; FED-BATCH CULTIVATION; PLASMINOGEN ACTIVATORS; PROKARYOTIC EXPRESSION SYSTEM; SPECIFIC ACTIVITY; TISSUE PLASMINOGEN ACTIVATOR; TISSUE-TYPE PLASMINOGEN ACTIVATOR;

ESCHERICHIA COLI;

FIBRIN; TISSUE PLASMINOGEN ACTIVATOR;

CHIMERA; COLIFORM BACTERIUM; FERMENTATION; GENE EXPRESSION; MICROBIAL ACTIVITY; PROTEIN; RECOMBINATION;

ARTICLE; BACTERIAL STRAIN; BINDING AFFINITY; CONTROLLED STUDY; ESCHERICHIA COLI; FED BATCH CULTURE; FERMENTATION; GENE CASSETTE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ESCHERICHIA COLI; PROKARYOTA;

EID: 84872414103     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12059     Document Type: Article

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