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Volumn 36, Issue 4, 2013, Pages 469-487

A kinetic-metabolic model based on cell energetic state: Study of CHO cell behavior under Na-butyrate stimulation

Author keywords

CHO cells; Energy regulation; Kinetic model; Metabolic modeling; Metabolic regulation; Sodium butyrate

Indexed keywords

AMINO ACIDS; CYTOLOGY; ENERGY POLICY; GLOBAL OPTIMIZATION; GLUCOSE; KINETICS; METABOLISM; PARAMETER ESTIMATION; SENSITIVITY ANALYSIS; SODIUM; SODIUM COMPOUNDS;

EID: 84880132740     PISSN: 16157591     EISSN: 16157605     Source Type: Journal    
DOI: 10.1007/s00449-012-0804-3     Document Type: Article
Times cited : (32)

References (56)
  • 1
    • 33745038944 scopus 로고    scopus 로고
    • Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells
    • DOI 10.1021/bp050339v
    • Galbraith DJ, Tait AS, Racher AJ, Birch JR, James DC (2006) Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells. Biotechnol Prog 22:753-762 (Pubitemid 43877444)
    • (2006) Biotechnology Progress , vol.22 , Issue.3 , pp. 753-762
    • Galbraith, D.J.1    Tait, A.S.2    Racher, A.J.3    Birch, J.R.4    James, D.C.5
  • 2
    • 0034281735 scopus 로고    scopus 로고
    • Insulin-like growth factor-i and transferrin mediate growth and survival of Chinese hamster ovary cells
    • Sunstrom NS, Gay RD, Wong DC, Kitchen NA, Deboer L, Gray PP (2000) Insulin-like growth factor-i and transferrin mediate growth and survival of Chinese hamster ovary cells. Biotechnol Prog 16:698-702
    • (2000) Biotechnol Prog , vol.16 , pp. 698-702
    • Sunstrom, N.S.1    Gay, R.D.2    Wong, D.C.3    Kitchen, N.A.4    Deboer, L.5    Gray, P.P.6
  • 3
    • 0031945008 scopus 로고    scopus 로고
    • Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells
    • DOI 10.1038/nbt0598-468
    • Fussenegger M, Schlatter S, Datwyler D, Mazur X, Bailey JE (1998) Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat Biotechnol 16:468-472 (Pubitemid 28211201)
    • (1998) Nature Biotechnology , vol.16 , Issue.5 , pp. 468-472
    • Fussenegger, M.1    Schlatter, S.2    Datwyler, D.3    Mazur, X.4    Bailey, J.E.5
  • 4
    • 77957018662 scopus 로고    scopus 로고
    • Gene amplification and vector engineering to achieve rapid and high-level therapeutic protein production using the Dhfr-based CHO cell selection system
    • Cacciatore JJ, Chasin LA, Leonard EF (2010) Gene amplification and vector engineering to achieve rapid and high-level therapeutic protein production using the Dhfr-based CHO cell selection system. Biotechnol Adv 28:673-681
    • (2010) Biotechnol Adv , vol.28 , pp. 673-681
    • Cacciatore, J.J.1    Chasin, L.A.2    Leonard, E.F.3
  • 6
    • 1942457733 scopus 로고    scopus 로고
    • Structural Requirements for Additional N-Linked Carbohydrate on Recombinant Human Erythropoietin
    • DOI 10.1074/jbc.M311095200
    • Elliott S, Chang D, Delorme E, Dunn C, Egrie J, Giffin J, Lorenzini T, Talbot C, Hesterberg L (2004) Structural requirements for additional N-linked carbohydrate on recombinant human erythropoietin. J Biol Chem 279:16854-16862 (Pubitemid 38509388)
    • (2004) Journal of Biological Chemistry , vol.279 , Issue.16 , pp. 16854-16862
    • Elliott, S.1    Chang, D.2    Delorme, E.3    Eris, T.4    Lorenzini, T.5
  • 9
    • 34250628439 scopus 로고    scopus 로고
    • Proliferation control strategies to improve productivity and survival during CHO based production culture
    • Kumar N, Gammell P, Clynes M (2007) Proliferation control strategies to improve productivity and survival during CHO based production culture. Cytotechnology 53:33-46
    • (2007) Cytotechnology , vol.53 , pp. 33-46
    • Kumar, N.1    Gammell, P.2    Clynes, M.3
  • 10
    • 33747370959 scopus 로고    scopus 로고
    • Antibody production
    • DOI 10.1016/j.addr.2005.12.006, PII S0169409X06000883
    • Birch JR, Racher AJ (2006) Antibody production. Adv Drug Deliv Rev 58:671-685 (Pubitemid 44247226)
    • (2006) Advanced Drug Delivery Reviews , vol.58 , Issue.5-6 , pp. 671-685
    • Birch, J.R.1    Racher, A.J.2
  • 11
    • 8344271026 scopus 로고    scopus 로고
    • Production of recombinant protein therapeutics in cultivated mammalian cells
    • Wurm FM (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 22:1393-1398
    • (2004) Nat Biotechnol , vol.22 , pp. 1393-1398
    • Wurm, F.M.1
  • 12
    • 34250642327 scopus 로고    scopus 로고
    • Modifications of therapeutic proteins: Challenges and prospects
    • Jenkins N (2007) Modifications of therapeutic proteins: challenges and prospects. Cytotechnology 53:121-125
    • (2007) Cytotechnology , vol.53 , pp. 121-125
    • Jenkins, N.1
  • 14
    • 78650525718 scopus 로고    scopus 로고
    • Dynamic model of CHO cell metabolism
    • Nolan RP, Lee K (2011) Dynamic model of CHO cell metabolism. Metab Eng 13:108-124
    • (2011) Metab Eng , vol.13 , pp. 108-124
    • Nolan, R.P.1    Lee, K.2
  • 15
    • 34547759811 scopus 로고    scopus 로고
    • Dynamic flux cartography of hairy roots primary metabolism
    • DOI 10.1016/j.phytochem.2007.04.028, PII S0031942207002907
    • Cloutier M, Perrier M, Jolicoeur M (2007) Dynamic flux cartography of hairy roots primary metabolism. Phytochemistry 68:2393-2404 (Pubitemid 47224960)
    • (2007) Phytochemistry , vol.68 , Issue.16-18 , pp. 2393-2404
    • Cloutier, M.1    Perrier, M.2    Jolicoeur, M.3
  • 16
    • 18244367173 scopus 로고    scopus 로고
    • Increased productivity of recombinant tissular plasminogen activator (t-PA) by butyrate and shift of temperature: A cell cycle phases analysis
    • DOI 10.1023/A:1014088919546
    • Hendrick V, Winnepenninckx P, Abdelkafi CV, Andeputte O, Cherlet M, Marique T, Renemann G, Loa A, Kretzmer G, Werenne J (2001) Increased productivity of recombinant tissular plasminogen activator (t-PA) by butyrate and shift of temperature: a cell cycle phases analysis. Cytotechnology 36:71-83 (Pubitemid 34067714)
    • (2001) Cytotechnology , vol.36 , Issue.1-3 , pp. 71-83
    • Hendrick, V.1    Winnepenninckx, P.2    Abdelkafi, C.3    Vandeputte, O.4    Cherlet, M.5    Marique, T.6    Renemann, G.7    Loa, A.8    Kretzmer, G.9    Werenne, J.10
  • 17
    • 0001162423 scopus 로고    scopus 로고
    • Overexpression of bcl-2 inhibits sodium butyrate-induced apoptosis in Chinese hamster ovary cells resulting in enhanced humanized antibody production
    • DOI 10.1002/1097-0290(2000)71:3<184::AID-BIT1008
    • Kim NS, Lee GM (2001) Overexpression of bcl-2 inhibits sodium butyrate-induced apoptosis in Chinese hamster ovary cells resulting in enhanced humanized antibody production. Biotechnol Bioeng 71:184-193 (Pubitemid 32381138)
    • (2000) Biotechnology and Bioengineering , vol.71 , Issue.3 , pp. 184-193
    • Kim, N.S.1    Lee, G.M.2
  • 18
    • 0038247891 scopus 로고    scopus 로고
    • Development of apoptosis-resistant dihydrofolate reductase-deficient Chinese hamster ovary cell line
    • DOI 10.1002/bit.10633
    • Lee SK, Lee GM (2003) Development of apoptosis resistant dihydrofolate reductase-deficient Chinese hamster ovary cell line. Biotechnol Bioeng 82:872-876 (Pubitemid 36604460)
    • (2003) Biotechnology and Bioengineering , vol.82 , Issue.7 , pp. 872-876
    • Lee, S.K.1    Lee, G.M.2
  • 19
    • 0025860171 scopus 로고
    • Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression
    • Palermo DP, DeGruf ME, Marotti KR, Rehberg E, Post LE (1992) Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression. J Biotechnol 19:35-48
    • (1992) J Biotechnol , vol.19 , pp. 35-48
    • Palermo, D.P.1    DeGruf, M.E.2    Marotti, K.R.3    Rehberg, E.4    Post, L.E.5
  • 20
  • 21
    • 0027112177 scopus 로고
    • Development of a helical-ribbon impeller bioreactor for high-density plant cell suspension culture
    • Jolicoeur M, Chavarie C, Carreau PJ, Archambault J (1992) Development of a helical-ribbon impeller bioreactor for high-density plant cell suspension culture. Biotechnol Bioeng 39:511-521
    • (1992) Biotechnol Bioeng , vol.39 , pp. 511-521
    • Jolicoeur, M.1    Chavarie, C.2    Carreau, P.J.3    Archambault, J.4
  • 22
    • 0025868196 scopus 로고
    • Improved ion-pair high-performance liquid chromatographic method for the quantification of a wide variety of nucleotides and sugar nucleotides in animal cells
    • Ryll T, Wagner R (1991) Improved ion-pair high-performance liquid chromatographic method for the quantification of a wide variety of nucleotides and sugar nucleotides in animal cells. J Chromatogr 570:77-88
    • (1991) J Chromatogr , vol.570 , pp. 77-88
    • Ryll, T.1    Wagner, R.2
  • 24
    • 78649447462 scopus 로고    scopus 로고
    • A detailed metabolic flux analysis of an underdetermined network of CHO cells
    • Zamoranoa F, Vande Wouwera A, Bastin G (2010) A detailed metabolic flux analysis of an underdetermined network of CHO cells. J Biotechnol 150:495-508
    • (2010) J Biotechnol , vol.150 , pp. 495-508
    • Zamoranoa, F.1    Vande Wouwera, A.2    Bastin, G.3
  • 25
    • 84555201597 scopus 로고    scopus 로고
    • Metabolic flux analysis of mammalian cells
    • Martens DE (2007) Metabolic flux analysis of mammalian cells. Cell Eng 5:275-299
    • (2007) Cell Eng , vol.5 , pp. 275-299
    • Martens, D.E.1
  • 26
    • 80052032471 scopus 로고    scopus 로고
    • Metabolic flux analysis of CHO cells at growth and non-growth phases using isotopic tracers and mass spectrometry
    • Ahn WC, Antoniewicz MR (2011) Metabolic flux analysis of CHO cells at growth and non-growth phases using isotopic tracers and mass spectrometry. Metab Eng 13:598-609
    • (2011) Metab Eng , vol.13 , pp. 598-609
    • Ahn, W.C.1    Antoniewicz, M.R.2
  • 27
    • 0019879459 scopus 로고
    • Control of energy transformation of mitochondria: Analysis by a quantitative model
    • Bohnensack R (1981) Control of energy transformation of mitochondria: analysis by a quantitative model. Biochim Biophys Act 634:203-218
    • (1981) Biochim Biophys Act , vol.634 , pp. 203-218
    • Bohnensack, R.1
  • 28
    • 0015047607 scopus 로고
    • Simulation of the Krebs cycle and closely related metabolism in perfused rat liver. I. Construction of a model
    • Garfinkel D (1971) Simulation of the Krebs cycle and closely related metabolism in perfused rat liver. I. Construction of a model. Comput Biomed Res 4:1-17
    • (1971) Comput Biomed Res , vol.4 , pp. 1-17
    • Garfinkel, D.1
  • 29
    • 0015045962 scopus 로고
    • Simulation of the Krebs cycle and closely related metabolism in perfused rat liver. II. Properties of the model
    • Garfinkel D (1971) Simulation of the Krebs cycle and closely related metabolism in perfused rat liver. II. Properties of the model. Comput Biomed Res 4:18-42
    • (1971) Comput Biomed Res , vol.4 , pp. 18-42
    • Garfinkel, D.1
  • 30
    • 0025995674 scopus 로고
    • An extended dynamic model of oxidative phosphorylation
    • Korzeniewski B (1991) An extended dynamic model of oxidative phosphorylation. Biochim Biophys Acta 1060:210-223
    • (1991) Biochim Biophys Acta , vol.1060 , pp. 210-223
    • Korzeniewski, B.1
  • 31
    • 34548297895 scopus 로고    scopus 로고
    • Computer modeling of mitochondrial tricarboxylic acid cycle, oxidative phosphorylation, metabolite transport, and electrophysiology
    • DOI 10.1074/jbc.M701024200
    • Wu F, Yang F, Vinnakota KC, Beard DA (2007) Computer modeling of mitochondrial tricarboxylic acid cycle, oxidative phosphorylation, metabolite transport, and electrophysiology. J Biol Chem 282:24525-24537 (Pubitemid 47347524)
    • (2007) Journal of Biological Chemistry , vol.282 , Issue.34 , pp. 24525-24537
    • Wu, F.1    Yang, F.2    Vinnakota, K.C.3    Beard, D.A.4
  • 32
    • 0035996969 scopus 로고    scopus 로고
    • Description and analysis of metabolic connectivity and dynamics in the human red blood cell
    • Kauffman LG, Pajerowski JD, Jamshidi N, Palsson BO, Edwards JS (2002) Description and analysis of metabolic connectivity and dynamics in the human red blood cell. Biophys J 83:646-662 (Pubitemid 34803606)
    • (2002) Biophysical Journal , vol.83 , Issue.2 , pp. 646-662
    • Kauffman, K.J.1    Pajerowski, J.D.2    Jamshidi, N.3    Palsson, B.O.4    Edwards, J.S.5
  • 33
    • 34249062728 scopus 로고    scopus 로고
    • A computational model of skeletal muscle metabolism linking cellular adaptations induced by altered loading states to metabolic responses during exercise
    • Dash RK, DiBella JA, Cabrera ME (2007) A computational model of skeletal muscle metabolism linking cellular adaptations induced by altered loading states to metabolic responses during exercise. Biomol Eng 6:14
    • (2007) Biomol Eng , vol.6 , pp. 14
    • Dash, R.K.1    DiBella, J.A.2    Cabrera, M.E.3
  • 35
    • 0035982810 scopus 로고    scopus 로고
    • A computational model for glycogenolysis in skeletal muscle
    • DOI 10.1114/1.1492813
    • Lambeth MJ, Kushmerick MJ (2002) A computational model for glycogenolysis in skeletal muscle. Ann Biomed Eng 30:808-827 (Pubitemid 35019468)
    • (2002) Annals of Biomedical Engineering , vol.30 , Issue.6 , pp. 808-827
    • Lambeth, M.J.1    Kushmerick, M.J.2
  • 36
    • 77049120710 scopus 로고    scopus 로고
    • Metabolic flux analysis of CHO cells in perfusion culture by metabolite balancing and 2D[13C, 1H] COSYNMR spectroscopy
    • Goudar C, Biener R, Boisart C, Heidemann R, Piret J, deGraaf A, Konstantinov K (2010) Metabolic flux analysis of CHO cells in perfusion culture by metabolite balancing and 2D[13C, 1H] COSYNMR spectroscopy. Metab Eng 12:138-149
    • (2010) Metab Eng , vol.12 , pp. 138-149
    • Goudar, C.1    Biener, R.2    Boisart, C.3    Heidemann, R.4    Piret, J.5    DeGraaf, A.6    Konstantinov, K.7
  • 37
    • 3242795003 scopus 로고    scopus 로고
    • The principle of flux minimization and its application to estimate stationary fluxes in metabolic networks
    • Holzhutter HG (2004) The principle of flux minimization and its application to estimate stationary fluxes in metabolic networks. Eur J Biochem 271:2905-2922
    • (2004) Eur J Biochem , vol.271 , pp. 2905-2922
    • Holzhutter, H.G.1
  • 38
    • 29244444547 scopus 로고    scopus 로고
    • Improvement of hepatitis B surface antigen expression by dimethyl sulfoxide in the culture of recombinant Chinese hamster ovary cells
    • Li JH, Sun XM, Zhang YX (2006) Improvement of hepatitis B surface antigen expression by dimethyl sulfoxide in the culture of recombinant Chinese hamster ovary cells. Process Biochem 41:317-322
    • (2006) Process Biochem , vol.41 , pp. 317-322
    • Li, J.H.1    Sun, X.M.2    Zhang, Y.X.3
  • 39
    • 0142092531 scopus 로고    scopus 로고
    • Determination of tricarboxylic acid cycle acids and other related substances in cultured mammalian cells by gradient ion-exchange chromatography with suppressed conductivity detection
    • DOI 10.1016/S0021-9673(03)01134-8
    • Lu S, Sun X, Shi H, Zhang Y (2003) Determination of tricarboxylic acid cycle acids and other related substances in cultured mammalian cells by gradient ion-exchange chromatography with suppressed conductivity detection. J Chromatogr A 1012:161-168 (Pubitemid 37297596)
    • (2003) Journal of Chromatography A , vol.1012 , Issue.2 , pp. 161-168
    • Lu, S.1    Sun, X.2    Shi, C.3    Zhang, Y.4
  • 40
    • 33748109526 scopus 로고    scopus 로고
    • Metabolic characteristics of recombinant Chinese hamster ovary cells expressing glutamine synthetase in presence and absence of glutamine
    • DOI 10.1007/s10616-006-9010-y
    • Zhang F, Sun W, Yi X, Zhang Y (2006) Metabolic characteristics of recombinant Chinese hamster ovary cells expressing glutamine synthetase in presence and absence of glutamine. Cytotechnology 51:21-28 (Pubitemid 44306072)
    • (2006) Cytotechnology , vol.51 , Issue.1 , pp. 21-28
    • Zhang, F.1    Sun, X.2    Yi, X.3    Zhang, Y.4
  • 41
    • 58149460015 scopus 로고    scopus 로고
    • Effective quenching processes for physiologically valid metabolite profiling of suspension cultured mammalian cells
    • Sellick CA, Hansen R, Maqsood AR, Dunn WB, Stephens GM, Goodacre R et al (2009) Effective quenching processes for physiologically valid metabolite profiling of suspension cultured mammalian cells. Anal Chem 81:174-183
    • (2009) Anal Chem , vol.81 , pp. 174-183
    • Sellick, C.A.1    Hansen, R.2    Maqsood, A.R.3    Dunn, W.B.4    Stephens, G.M.5    Goodacre, R.6
  • 42
  • 43
    • 77952065243 scopus 로고    scopus 로고
    • Metabolic effects of influenza virus infection in cultured animal cells: Intra- and extracellular metabolite profiling
    • Ritter JB, Wahl AS, Freund S, Genzel Y, Reichl U (2010) Metabolic effects of influenza virus infection in cultured animal cells: intra- and extracellular metabolite profiling. BMC Syst Biol 4:61
    • (2010) BMC Syst Biol , vol.4 , pp. 61
    • Ritter, J.B.1    Wahl, A.S.2    Freund, S.3    Genzel, Y.4    Reichl, U.5
  • 44
    • 13844296832 scopus 로고    scopus 로고
    • Insight into metabolism of CHO cells at low glucose concentration on the basis of the determination of intracellular metabolites
    • DOI 10.1016/j.procbio.2004.07.004, PII S0032959204003322
    • Lu S, Sun X, Zhang Y (2005) Insight into metabolism of CHO cells at low glucose concentration on the basis of the determination of intracellular metabolites. Process Biochem 40:1917-1921 (Pubitemid 40240870)
    • (2005) Process Biochemistry , vol.40 , Issue.5 , pp. 1917-1921
    • Lu, S.1    Sun, X.2    Zhang, Y.3
  • 46
    • 33846839360 scopus 로고    scopus 로고
    • Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment
    • DOI 10.1263/jbb.103.82, PII S1389172307700282
    • De Leon GM, Wlaschin KF, Nissom PM, Yap M, Hu WS (2007) Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment. J Biosci Bioeng 103:82-91 (Pubitemid 46216907)
    • (2007) Journal of Bioscience and Bioengineering , vol.103 , Issue.1 , pp. 82-91
    • De Leon, G.M.1    Wlaschin, K.F.2    Nissom, P.M.3    Yap, M.4    Hu, W.-S.5
  • 47
    • 41249093488 scopus 로고    scopus 로고
    • Genomic and proteomic exploration of CHO and hybridoma cells under sodium butyrate treatment
    • DOI 10.1002/bit.21665
    • Yee JC, De Leon GM, Philp RJ, Yap M, Hu WS (2008) Genomic and proteome exploration of CHO and hybridoma cells under sodium butyrate treatment. Biotechnol Bioeng 99:1186-1204 (Pubitemid 351462776)
    • (2008) Biotechnology and Bioengineering , vol.99 , Issue.5 , pp. 1186-1204
    • Joon, C.Y.1    Gatti, M.D.L.2    Philp, R.J.3    Yap, M.4    Hu, W.-S.5
  • 48
    • 0027971850 scopus 로고
    • Modulation of cellular proliferation alters glutamine transport and metabolism in human hepatoma cells
    • Bode BP, Souba WW (1994) Modulation of cellular proliferation alters glutamine transport and metabolism in human hepatoma cells. Ann Surg 220:411-424 (Pubitemid 24323438)
    • (1994) Annals of Surgery , vol.220 , Issue.4 , pp. 411-424
    • Bode, B.P.1    Souba, W.W.2
  • 49
    • 0032521113 scopus 로고    scopus 로고
    • The characterization of butyrate transport across pig and human colonic luminal membrane
    • DOI 10.1111/j.1469-7793.1998.819bs.x
    • Rizhaupt A, Ellis A, Hosie KB, P Shirazi-Beechey S (1998) The characterization of butyrate transport across pig and human colonic luminal membrane. J Physiol 507:819-830 (Pubitemid 28143582)
    • (1998) Journal of Physiology , vol.507 , Issue.3 , pp. 819-830
    • Ritzhaupt, A.1    Ellis, A.2    Hosie, K.B.3    Shirazi-Beechey, S.P.4
  • 50
    • 0034938816 scopus 로고
    • Butyrate impairs energy metabolism in isolated perfused liver of fed rats
    • Beauvieux MC, Tissier P, Gin H, Canioni P, Gallis JL (1986) Butyrate impairs energy metabolism in isolated perfused liver of fed rats. J Nutr 131:1986-1992
    • (1986) J Nutr , vol.131 , pp. 1986-1992
    • Beauvieux, M.C.1    Tissier, P.2    Gin, H.3    Canioni, P.4    Gallis, J.L.5
  • 52
    • 0033587379 scopus 로고    scopus 로고
    • Metabolic flux analysis of hybridoma continuous culture steady state multiplicity
    • DOI 10.1002/(SICI)1097-0290(19990620)63:6<675::AID
    • Follstad BD, Balcarel RR, Stephanopoulos G, Wang D (1999) Metabolic flux analysis of hybridoma continuous culture steady state multiplicity. Biotechnol Bioeng 63:675-683 (Pubitemid 29200402)
    • (1999) Biotechnology and Bioengineering , vol.63 , Issue.6 , pp. 675-683
    • Follstad, B.D.1    Balcarcel, R.R.2    Stephanopoulos, G.3    Wang, D.I.C.4
  • 54
    • 44449177159 scopus 로고    scopus 로고
    • 13C-labeling experiments: Part II. Flux estimation
    • DOI 10.1002/bit.21746
    • Maier K, Hofmann U, Reuss M, Mauch K (2008) Identification of metabolic fluxes in hepatic cells from transient 13C-labeling experiments: part II. Flux estimation. Biotechnol Bioeng 100:355-370 (Pubitemid 351758177)
    • (2008) Biotechnology and Bioengineering , vol.100 , Issue.2 , pp. 355-370
    • Maier, K.1    Hofmann, U.2    Reuss, M.3    Mauch, K.4
  • 55
    • 0035543345 scopus 로고    scopus 로고
    • Analysis of CHO cells metabolic redistribution in a glutamate-based defined medium in continuous culture
    • DOI 10.1021/bp0100981
    • Altamirano C, Illanes A, Casablancas A, Gámez X, Cairó JJ, Gòdia C (2001) Analysis of CHO cells metabolic redistribution in a glutamate-based defined medium in continuous culture. Biotechnol Prog 17:1032-1041 (Pubitemid 34018281)
    • (2001) Biotechnology Progress , vol.17 , Issue.6 , pp. 1032-1041
    • Altamirano, C.1    Illanes, A.2    Casablancas, A.3    Gamez, X.4    Cairo, J.J.5    Godia, C.6


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