Identification of metabolic fluxes in hepatic cells from transient 13C-labeling experiments: Part I. Experimental observations

Biotechnology and Bioengineering

Volumn 100, Issue 2, 2008, Pages 344-354

  Hofmann, Ute ^a   Maier, Klaus ^b   Niebel, Anja ^b   Vacun, Gabriele ^b   Reuss, Matthias ^b   Mauch, Klaus ^c  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b UNIVERSITY OF STUTTGART   (Germany)

^c INSILICO Biotechnology GmbH   (Germany)

Author keywords

13C labeling; Extraction; Hepatic metabolism; Intracellular metabolite concentrations; Mammalian cells; Quantification

Indexed keywords

AMINO ACIDS; CELL CULTURE; COLLAGEN; GLUCOSE; LIQUID CHROMATOGRAPHY;

HEPATIC CELLS; MAMMALIAN CELLS; METABOLIC FLUXES;

METABOLISM;

AMINO ACID; CARBON 13; COLLAGEN; GLUCOSE; LACTIC ACID; PENTOSE PHOSPHATE; PYRUVIC ACID; TRICARBOXYLIC ACID; UREA;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL METABOLISM; EXCRETION; EXTRACTION; GAS CHROMATOGRAPHY; GLYCOLYSIS; LIQUID CHROMATOGRAPHY; LIVER CELL; MASS SPECTROMETRY; METABOLITE; NONHUMAN;

CARBON RADIOISOTOPES; CELL LINE; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HEPATOCYTES; HUMANS; ISOTOPE LABELING; SIGNAL TRANSDUCTION;

MAMMALIA;

EID: 44449113808     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21747     Document Type: Article

References (29)

1. Bhattacharya M, Fuhrman L, Ingram A, Nickerson KW, Conway T. 1995. Single-run separation and detection of multiple metabolic intermediates by anion-exchange high-performance liquid chromatography and application to cell pool extracts prepared from Escherichia coli. Anal Biochem 232(1):98-106.
2. Buchholz A, Takors R, Wandrey C. 2001. Quantification of intracellular metabolites in Escherichia coli K12 using liquid chromatographic-electrospray ionization tandem mass spectrometric techniques. Anal Biochem 295(2):129-137.
3. Chaves Das Neves HJ, Vasconcelos AM. 1987. Capillary gas chromatography of amino acids, including asparagine and glutamine: Sensitive gas chromatographic-mass spectrometric and selected ion monitoring gas chromatographic-mass spectrometric detection of the N,O(S)-tert- butyldimethylsilyl derivatives. J Chromatogr 392:249-258.
4. Des Rosiers C, Di Donato L, Comte B, Laplante A, Marcoux C, David F, Fernandez CA, Brunengraber H. 1995. Isotopomer analysis of citric acid cycle and gluconeogenesis in rat liver. Reversibility of isocitrate dehydrogenase and involvement of ATP-citrate lyase in gluconeogenesis. J Biol Chem 270(17):10027-10036.
5. Doverskog M, Ljunggren J, Ohman L, Haggstrom L. 1997. Physiology of cultured animal cells. J Biotechnol 59(1-2):103-115.
6. Fischer E, Scheibler H. 1909. Zur Kenntnis der Waldenschen Umkehrung. IV. Ber 42:1219-1228.
7. 13C constraints. Anal Biochem 325(2):308-316.
8. Goel A, Ferrance J, Jeong J, Ataai MM. 1993. Analysis of metabolic fluxes in batch and continous cultures of Bacillus subtilis. Biotechnol Bioeng 42:686-696.
9. Gonzalez B, Francois J, Renaud M. 1997. A rapid and reliable method for metabolite extraction in yeast using boiling buffered ethanol. Yeast 13(14):1347-1355.
10. Harvey DJ, Horning MG. 1973. Characterization of the trimethylsilyl derivatives of sugar phosphates and related compounds by gas chromatography and gas chromatography-mass spectrometry. J Chromatogr 76(1):51-62.
11. Hermes M, von Hippel S, Osswald H, Kloor D. 2005. S-adenosylhomocysteine metabolism in different cell lines: Effect of hypoxia and cell density. Cell Physiol Biochem 15(5):233-244.
12. Hua Q, Yang C, Baba T, Mori H, Shimizu K. 2003. Responses of the central metabolism in Escherichia coli to phosphoglucose isomerase and glucose-6-phosphate dehydrogenase knockouts. J Bacteriol 185(24):7053-7067.
13. Kury D, Keller U. 1991. Trimethylsilyl-O-methyloxime derivatives for the measurement of [6,6-2H2]-D-glucose-enriched plasma samples by gas chromatography-mass spectrometry. J Chromatogr 572(1-2):302-306.
14. Lee K, Berthiaume F, Stephanopoulos GN, Yarmush ML. 1999. Metabolic flux analysis: A powerful tool for monitoring tissue function. Tissue Eng 5(4):347-368.
15. Luo B, Groenke K, Takors R, Wandrey C, Oldiges M. 2007. Simultaneous determination of multiple intracellular metabolites in glycolysis, pentose phosphate pathway and tricarboxylic acid cycle by liquid chromatography-mass spectrometry. J Chromatogr A 1147(2):153-164.
16. Maharjan RP, Ferenci T. 2003. Global metabolite analysis: The influence of extraction methodology on metabolome profiles of Escherichia coli. Anal Biochem 313(1):145-154.
17. 13C labeling experiments. J Biotechnol 129(2):249-267.
18. 13C and 2H mass isotopomer distribution of phosphoenolpyruvate by gas chromatography/mass spectrometry. J Mass Spectrom 31(6):643-648.
19. 13C-based flux analysis. Mol Syst Biol 2:62.
20. 13C tracer experiments and metabolite balancing for metabolic flux analysis: Comparing two approaches. Biotechnol Bioeng 58(2-3):254-267.
21. van Dam JC, Eman MR, Frank J, Lange HC, van Dedem GWK, Heijnen JJ. 2002. Analysis of glycolytic intermediates in Saccharomyces cerevisiae using anion exchange chromatography and electrospray ionization with tandem mass spectrometric detection. Anal Chim Acta 460:209-218.
22. 13C-labeled primary metabolites. FEMS Yeast Res 5(6-7):559-568.
23. Villas-Boas SG, Mas S, Akesson M, Smedsgaard J, Nielsen J. 2005. Mass spectrometry in metabolome analysis. Mass Spectrom Rev 24(5):613-646.
24. 13C metabolic flux analysis. Metab Eng 3(3):195-206.
25. Wiechert W, Nöh K. 2005. From stationary to instationary metabolic flux analysis. Adv Biochem Eng Biotechnol 92:145-172.
26. Wittmann C, Hans M, van Winden WA, Ras C, Heijnen JJ. 2005. Dynamics of intracellular metabolites of glycolysis and TCA cycle during cell-cycle-related oscillation in Saccharomyces cerevisiae. Biotechnol Bioeng 89(7):839-847.
27. Xie L, Wang DI. 1994. Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor. Cytotechnology 15(1-3):17-29.
28. Yang MS, Yu LC, Gupta RC. 2004. Analysis of changes in energy and redox states in HepG2 hepatoma and C6 glioma cells upon exposure to cadmium. Toxicology 201(1-3):105-113.
29. Yarmush ML, Banta S. 2003. Metabolic engineering: Advances in modeling and intervention in health and disease. Annu Rev Biomed Eng 5:349-381.