Metabolite-balancing techniques vs. 13C tracer experiments to determine metabolic fluxes in hybridoma cells

Biotechnology and Bioengineering

Volumn 58, Issue 2-3, 1998, Pages 258-262

  Bonarius, Hendrik P J ^a,b   Timmerarends, Bram ^a   De Gooijer, Cornelis D ^a   Tramper, Johannes ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b NOVO NORDISK A S   (Denmark)

Author keywords

13C tracer; Hybridoma; Mass balance; Mass spectroscopy; Metabolic flux; NMR spectroscopy

Indexed keywords

ADENOSINETRIPHOSPHATE; CONTINUOUS CELL CULTURE; MASS SPECTROMETRY; METABOLISM; METABOLITES; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

MASS BALANCE; METABOLIC FLUX;

ANIMAL CELL CULTURE;

ADENOSINE TRIPHOSPHATE; CARBON 13; CARBON DIOXIDE; GLUCOSE; LACTIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYBRIDOMA; MASS SPECTROMETRY; METABOLISM; METABOLITE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTON NUCLEAR MAGNETIC RESONANCE;

ADENOSINE TRIPHOSPHATE; ANIMALS; CARBON ISOTOPES; CELLS, CULTURED; COMPUTATIONAL BIOLOGY; HYBRIDOMAS; MASS SPECTROMETRY; MODELS, BIOLOGICAL; NAD; NADP;

ANIMALIA; MAMMALIA;

EID: 0032485839     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980420)58:2/3<258::AID-BIT20>3.0.CO;2-7     Document Type: Article

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