Evaluation of PCR amplification bias by terminal restriction fragment length polymorphism analysis of small-subunit rRNA and mcrA genes by using defined template mixtures of methanogenic pure cultures and soil DNA extracts

Applied and Environmental Microbiology

Volumn 69, Issue 1, 2003, Pages 320-326

  Lueders, Tillmann ^a   Friedrich, Michael W ^a  

^a M P Inst Terrestrische Mikrobiol   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COENZYMES; DNA; FLUORESCENCE; RNA;

MICROBIAL COMMUNITY ANALYSIS;

MICROBIOLOGY;

GENOMIC DNA; METHYL COENZYME M REDUCTASE; PRIMER DNA; PROTEIN; SMALL SUBUNIT RIBOSOMAL RNA; UNCLASSIFIED DRUG;

POLYMERASE CHAIN REACTION;

AMPLICON; ARTICLE; BACTERIUM CULTURE; DNA EXTRACTION; DNA TEMPLATE; EVALUATION; FLUORESCENCE; GENE AMPLIFICATION; GENE FREQUENCY; METHANOBACTERIALES; NONHUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; QUANTITATIVE GENETICS; REPRODUCIBILITY; RESTRICTION FRAGMENT; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SOIL; TECHNIQUE; TEMPERATURE;

BIAS (EPIDEMIOLOGY); CULTURE MEDIA; DNA, BACTERIAL; ECOSYSTEM; EURYARCHAEOTA; OXIDOREDUCTASES; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL; SOIL; TEMPLATES, GENETIC;

BACTERIA (MICROORGANISMS); METHANOBACTERIALES;

EID: 0037225001     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.1.320-326.2003     Document Type: Article

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