Activation of p115-RhoGEF requires direct association of Gα13and the Dbl homology domain

Journal of Biological Chemistry

Volumn 287, Issue 30, 2012, Pages 25490-25500

  Chen, Zhe ^a   Guo, Liang ^b   Hadas, Jana ^a   Gutowski, Stephen ^a   Sprang, Stephen R ^c   Sternweis, Paul C ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b ARGONNE NATIONAL LABORATORY   (United States)

^c UNIVERSITY OF MONTANA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIRECT LINKS; G PROTEIN; GTPASES; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HELICAL DOMAINS; HETEROTRIMERIC G PROTEINS; HOMOLOGY DOMAIN; HYDROPHOBIC MOTIFS; PLECKSTRIN HOMOLOGY DOMAINS; SINGLE TRYPTOPHANS; SMALL ANGLE X-RAY SCATTERING;

AMINO ACIDS; X RAY SCATTERING;

BINDING SITES;

G PROTEIN ALPHA13; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; PROTEIN DBL; PROTEIN P155; REGULATOR FOR G PROTEIN SIGNALING; REGULATOR PROTEIN; RHO KINASE; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; BINDING SITE; BIOCHEMISTRY; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; HYDROPHOBICITY; MUTAGENESIS; MUTATION; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; RADIATION SCATTERING; REGULATORY MECHANISM; SEQUENCE HOMOLOGY;

AMINO ACID MOTIFS; BINDING SITES; GTP-BINDING PROTEIN ALPHA SUBUNITS, G12-G13; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MUTAGENESIS; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; RHOA GTP-BINDING PROTEIN; SIGNAL TRANSDUCTION; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84864076260     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.333716     Document Type: Article

References (35)

1. Sternweis, P. C., Carter, A. M., Chen, Z., Danesh, S. M., Hsiung, Y. F., and Singer, W. D. (2007) Regulation of Rho guanine nucleotide exchange factors by G proteins. Adv. Protein Chem. 74, 189-228 (Pubitemid 47369751)
2. Aittaleb, M., Boguth, C. A., and Tesmer, J. J. (2010) Structure and function of heterotrimeric G protein-regulated Rho guanine nucleotide exchange factors. Mol. Pharmacol. 77, 111-125
3. Etienne-Manneville, S., and Hall, A. (2002) Rho GTPases in cell biology. Nature 420, 629-635 (Pubitemid 36764489)
4. Rossman, K. L., Der, C. J., and Sondek, J. (2005) GEF means go. Turning on RHO GTPases with guanine nucleotide-exchange factors. Nat. Rev. Mol. Cell Biol. 6, 167-180.
5. Schmidt, A., and Hall, A. (2002) Guanine nucleotide exchange factors for Rho GTPases. Turning on the switch. Genes Dev. 16, 1587-1609 (Pubitemid 34743321)
6. Kozasa, T., Jiang, X., Hart, M. J., Sternweis, P. M., Singer, W. D., Gilman, A. G., Bollag, G., and Sternweis, P. C. (1998) p115 RhoGEF, a GTPase activating protein for Ga12 and Ga13. Science 280, 2109-2111
7. Hart, M. J., Jiang, X., Kozasa, T., Roscoe, W., Singer, W. D., Gilman, A. G., Sternweis, P. C., and Bollag, G. (1998) Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Ga13. Science 280, 2112-2114 (Pubitemid 28366203)
8. 12 activates Rho GTPase through tyrosine-phosphorylated leukemia-associated Rho- GEF. Proc. Natl. Acad. Sci. U.S.A. 100, 733-738
9. 13 with significant conformational rearrangements in the interface. J. Biol. Chem. 284, 5000-5009
10. Taya, S., Inagaki, N., Sengiku, H., Makino, H., Iwamatsu, A., Urakawa, I., Nagao, K., Kataoka, S., and Kaibuchi, K. (2001) Direct interaction of insulin- like growth factor-1 receptor with leukemia-associated RhoGEF. J. Cell Biol. 155, 809-820
11. Aurandt, J., Vikis, H. G., Gutkind, J. S., Ahn, N., and Guan, K. L. (2002) The semaphorin receptor plexin-B1 signals through a direct interaction with the Rho-specific nucleotide exchange factor, LARG. Proc. Natl. Acad. Sci. U.S.A. 99, 12085-12090
12. Swiercz, J. M., Kuner, R., Behrens, J., and Offermanns, S. (2002) Plexin-B1 directly interacts with PDZ-RhoGEF/LARG to regulate RhoA and growth cone morphology. Neuron 35, 51-63 (Pubitemid 34804322)
13. Chen, Z., Singer, W. D., Danesh, S. M., Sternweis, P. C., and Sprang, S. R. (2008) Recognition of the activated states of Gα13 by the rgRGS domain of PDZRhoGEF. Structure 16, 1532-1543
14. Chen, Z., Singer, W. D., Sternweis, P. C., and Sprang, S. R. (2005) Structure of the p115RhoGEF rgRGS domain-Gα13/i1 chimera complex suggests convergent evolution of a GTPase activator. Nat. Struct. Mol. Biol. 12, 191-197
15. Chen, Z., Wells, C. D., Sternweis, P. C., and Sprang, S. R. (2001) Structure of the rgRGS domain of p115RhoGEF. Nat. Struct. Biol. 8, 805-809 (Pubitemid 32803601)
16. Longenecker, K. L., Lewis, M. E., Chikumi, H., Gutkind, J. S., and Derewenda, Z. S. (2001) Structure of the RGS-like domain from PDZ-RhoGEF. Linking heterotrimeric G protein-coupled signaling to Rho GTPases. Structure 9, 559-569.
17. Wells, C. D., Liu, M. Y., Jackson, M., Gutowski, S., Sternweis, P. M., Rothstein, J. D., Kozasa, T., and Sternweis, P. C. (2002) Mechanisms for reversible regulation between p115 RhoGEF and GTRAP48 and the G12 class of heterotrimeric G proteins. J. Biol. Chem. 277, 1174-1181 (Pubitemid 34968865)
18. Chen, Z., Singer, W. D., Wells, C. D., Sprang, S. R., and Sternweis, P. C. (2003) Mapping the Gα13 binding interface of the rgRGS domain of p115RhoGEF. J. Biol. Chem. 278, 9912-9919 (Pubitemid 36800495)
19. Chen, Z., Medina, F., Liu, M. Y., Thomas, C., Sprang, S. R., and Sternweis, P. C. (2010) Activated RhoA Binds to the pleckstrin homology (PH) domain of PDZ-RhoGEF, a potential site for autoregulation. J. Biol. Chem. 285, 21070-21081
20. Chen, Z., Guo, L., Sprang, S. R., and Sternweis, P. C. (2011) Modulation of a GEF switch. Autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF. Protein Sci. 20, 107-117
21. Semenyuk, A. V., and Svergun, D. I. (1991) Gnom. A program package for small-angle scattering data-processing. J. Appl. Crystallogr. 24, 537-540
22. Svergun, D. I., Petoukhov, M. V., and Koch, M. H. (2001) Determination of domain structure of proteins from x-ray solution scattering. Biophys. J. 80, 2946-2953 (Pubitemid 32521666)
23. Volkov, V. V., and Svergun, D. I. (2003) Uniqueness of ab initio shape determination in small-angle scattering. J. Appl. Crystallogr. 36, 860-864
24. Wriggers, W. (2010) Using Situs for the integration of multi-resolution structures. Biophys. Rev. 2, 21-27
25. Pettersen, E. F., Goddard, T. D., Huang, C. C., Couch, G. S., Greenblatt, D. M., Meng, E. C., and Ferrin, T. E. (2004) UCSF chimera. A visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605-1612
26. 2+ sensitization pathway in smooth muscle. Structure 12, 1955-1965
27. Kristelly, R., Gao, G., and Tesmer, J. J. (2004) Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine- nucleotide exchange factor. J. Biol. Chem. 279, 47352-47362 (Pubitemid 39518392)
28. Laurie, A. T., and Jackson, R. M. (2005) Q-SiteFinder. An energy-based method for the prediction of protein-ligand binding sites. Bioinformatics 21, 1908-1916 (Pubitemid 40668026)
29. Schneidman-Duhovny, D., Inbar, Y., Nussinov, R., and Wolfson, H. J. (2005) PatchDock and SymmDock. Servers for rigid and symmetric docking. Nucleic Acids Res. 33, W363-W367 (Pubitemid 44529944)
30. Bielnicki, J. A., Shkumatov, A. V., Derewenda, U., Somlyo, A. V., Svergun, D. I., and Derewenda, Z. S. (2011) Insights into the molecular activation mechanism of the RhoA-specific guanine nucleotide exchange factor, PDZRhoGEF. J. Biol. Chem. 286, 35163-35175
31. Zheng, M., Cierpicki, T., Momotani, K., Artamonov, M. V., Derewenda, U., Bushweller, J. H., Somlyo, A. V., and Derewenda, Z. S. (2009) On the mechanism of autoinhibition of the RhoA-specific nucleotide exchange factor PDZRhoGEF. BMC Struct. Biol. 9, 36
32. Lutz, S., Shankaranarayanan, A., Coco, C., Ridilla, M., Nance, M. R., Vettel, C., Baltus, D., Evelyn, C. R., Neubig, R. R., Wieland, T., and Tesmer, J. J. (2007) Structure of Gαq-p63RhoGEF-RhoA complex reveals a pathway for the activation of RhoA by GPCRs. Science 318, 1923-1927
33. Kimple, R. J., Kimple, M. E., Betts, L., Sondek, J., and Siderovski, D. P. (2002) Structural determinants for GoLoco-induced inhibition of nucleotide release by Gα subunits. Nature 416, 878-881 (Pubitemid 34453747)
34. Kreutz, B., Yau, D. M., Nance, M. R., Tanabe, S., Tesmer, J. J., and Kozasa, T. (2006) A new approach to producing functional Gα subunits yields the activated and deactivated structures of Gα12/13 proteins. Biochemistry 45, 167-174 (Pubitemid 43054094)
35. Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T. J., Higgins, D. G., and Thompson, J. D. (2003) Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res. 31, 3497-3500 (Pubitemid 37442184)