Available methods for assembling expression cassettes for synthetic biology

Applied Microbiology and Biotechnology

Volumn 93, Issue 5, 2012, Pages 1853-1863

  Wang, Tianwen ^a   Ma, Xingyuan ^b   Zhu, Hu ^c   Li, Aitao ^b   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

^b EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c CHINA UNIVERSITY OF PETROLEUM   (China)

Author keywords

Acembl system; Cre loxP site specific recombination; Pathway construction; Simultaneous expression; Synthetic biology; Yeast recombination

Indexed keywords

ACEMBL SYSTEM; CRE-LOXP; DNA MOLECULES; EXPRESSION CASSETTE; EXPRESSION SYSTEM; IN-VITRO; IN-VIVO; MULTICOMPONENTS; OVER-EXPRESSION; PROTEIN COMPLEXES; SIMULTANEOUS EXPRESSION; STRUCTURAL BIOLOGY; SYNTHETIC BIOLOGY;

CLONING; YEAST;

BIOLOGY;

CHROMOSOME; CLONE; ENZYME ACTIVITY; MITOCHONDRIAL DNA; PROTEIN; RECOMBINATION; YEAST;

CLONING; GENETIC RECOMBINATION; LIGATION INDEPENDENT CLONING; METABOLIC ENGINEERING; MOLECULAR CLONING; PLASMID; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SHORT SURVEY; SYNTHETIC BIOLOGY;

GENE EXPRESSION; GENETIC ENGINEERING; GENETIC VECTORS; GENETICS, MICROBIAL; MUTAGENESIS, INSERTIONAL; PLASMIDS; RECOMBINATION, GENETIC; SYNTHETIC BIOLOGY; YEASTS;

EID: 84862793012     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-3920-8     Document Type: Short Survey

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