Efficient co-expression of a recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli

Biochemical Journal

Volumn 385, Issue 1, 2005, Pages 181-187

  Wladyka, Benedykt ^a   Puzia, Katarzyna ^a   Dubin, Adam ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

Co expression; Cysteine protease; Inhibitor; Refolding; Staphopain A; Staphostatin A

Indexed keywords

AFFINITY CHROMATOGRAPHY; DNA; ESCHERICHIA COLI; GENES; MUTAGENESIS; NATURAL RESOURCES; TOXICITY;

MUTATIONS; PROTEOLYTIC ACTIVITY; STAPHOSTATIN A; STAPHYLOCOCCAL CYSTEINE PROTEASE;

ENZYMES;

CYSTEINE PROTEINASE; CYSTEINE PROTEINASE INHIBITOR; GENOMIC DNA; RECOMBINANT ENZYME; STAPHOPAIN A; STAPHOSTATIN A; UNCLASSIFIED DRUG;

ARTICLE; CONFORMATIONAL TRANSITION; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FOLDING; STAPHYLOCOCCUS AUREUS; STRUCTURE ACTIVITY RELATION;

BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; CYSTEINE ENDOPEPTIDASES; CYSTEINE PROTEINASE INHIBITORS; ESCHERICHIA COLI; GENE EXPRESSION; MUTATION; PROTEIN RENATURATION; RECOMBINANT PROTEINS; STAPHYLOCOCCUS AUREUS;

ESCHERICHIA COLI; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; VECTORS;

EID: 12744279612     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040958     Document Type: Article

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