General method for plasmid construction using homologous recombination

BioTechniques

Volumn 26, Issue 1, 1999, Pages 134-141

  Raymond, Christopher K ^a   Pownder, Tracey A ^a   Sexson, Shannon L ^a  

^a ZYMOGENETICS INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; ESCHERICHIA COLI; EXCISION REPAIR; EXPRESSION VECTOR; GENETIC RECOMBINATION; NONHUMAN; PROTEIN EXPRESSION; RECOMBINANT PLASMID; SACCHAROMYCES CEREVISIAE; TECHNIQUE;

ESCHERICHIA COLI; MAMMALIA; SACCHAROMYCES CEREVISIAE;

EID: 0032893534     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99261rr02     Document Type: Article

References (20)

1. Boulton, S. and S. Jackson. 1996. Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break joining and in telomeric maintenance. Nucleic Acids Res. 24:4639-4648.
2. Casadaban, M. and S. Cohen. 1980. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138:179-207.
3. Cormack, B., R. Valdivia and S. Falkow. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38.
4. Evan, G., G. Lewis, G. Ramsay and J. Bishop. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5:3610-3616.
5. Hoffman, C. and F. Winston. 1987. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57:267-272.
6. Hudson, J., Jr., E. Dawson, K. Rushing, C. Jackson, D. Lockshon, D. Conover, C. Lanciault, J. Harris, S. Simmons, R. Rothstein and S. Fields. 1997. The complete set of predicted genes from Saccharomyces cerevisiae in a readily usable form. Genome Res. 7:1169-1173.
7. Kunes, S., H. Ma, K. Overbye, M. Fox and D. Botstein. 1987. Fine structure recombinational analysis of cloned genes using yeast transformation. Genetics 115:73-81.
8. Kurjan, J. and I. Herskowitz. 1982. Structure of a yeast pheromone gene (Mfα): a putative α-factor precursor contains four tandem copies of mature α-factor. Cell 39:933-943.
9. Ma, H., S. Kunes, P. Schatz and D. Botstein. 1987. Plasmid construction by homologous recombination in yeast. Gene 58:201-216.
10. Marykwas, D. and S. Passmore. 1995. Mapping by multifragment cloning in vivo. Proc. Natl. Acad. Sci. USA 92:11701-11705.
11. Meilhoc, E., J.-M. Masson and J. Teisse. 1990. High efficiency transformation of intact yeast cells by electric field pulses. Bio/Technology 8:223-227.
12. Mezard, C. and A. Nicolas. 1994. Homologous, homeologous, and illegitimate repair of double-strand breaks during transformation of a wild-type strain and a rad52 mutant strain of Saccharomyces cerevisiae. Mol. Cel. Biol. 14:1278-1292.
13. Mezard, C., D. Pompon and A. Nicolas. 1992. Recombination between similar but not identical DNA sequences during yeast transformation occurs within short stretches of identity. Cell 70:659-670.
14. Oldenburg, K., K. Vo, S. Michaelis and C. Paddon. 1997. Recombination-mediated PCR-directed plasmid construction in vivo in yeast. Nucleic Acids Res. 25:451-452.
15. Orr-Weaver, T. and R. Szostak. 1985. Fungal recombination. Microbiol. Rev. 49:33-58.
16. Raymond, C., T. Bukowski, S. Holderman, A. Ching, E. Vanaja and M. Stamm. 1998. Development of the methylotrophic yeast Pichia methanolica for the expression of the 65 kilodalton isoform of human glutamate decarboxylase. Yeast 14:11-23.
17. Rothman, J., I. Howald and T. Stevens. 1989. Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J. 8:2057-2065.
18. Schiestl, R. and R. Gietz. 1989. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr. Genet. 16:339-346.
19. Sikorski, R. and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisisae. Genetics 122:19-27.
20. Zhang, Y., R. Proenca, M. Maffei, M. Barone, L. Leopold and J. Friedman. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 372:425-427.