Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome

Science

Volumn 319, Issue 5867, 2008, Pages 1215-1220

  Gibson, Daniel G ^a   Benders, Gwynedd A ^a   Andrews Pfannkoch, Cynthia ^a   Denisova, Evgeniya A ^a   Baden Tillson, Holly ^a   Zaveri, Jayshree ^a   Stockwell, Timothy B ^a   Brownley, Anushka ^a   Thomas, David W ^a   Algire, Mikkel A ^a   Merryman, Chuck ^a   Young, Lei ^a   Noskov, Vladimir N ^a   Glass, John I ^a   Venter, J Craig ^a   Hutchison III, Clyde A ^a   Smith, Hamilton O ^a  

^a J CRAIG VENTER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHODIESTERASE I; TAQ POLYMERASE;

BACTERIUM; CHEMICAL ANALYSIS; CHROMOSOME; CLONE; DNA; GENOME; PARASITE; PATHOGENICITY;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; BACTERIAL GENOME; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE SEQUENCE; MOLECULAR CLONING; MYCOPLASMA GENITALIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT; SACCHAROMYCES CEREVISIAE; SYNTHESIS; TRANSPOSON; YEAST CELL;

BASE SEQUENCE; CHROMOSOMES, ARTIFICIAL, BACTERIAL; CHROMOSOMES, ARTIFICIAL, YEAST; CLONING, MOLECULAR; DNA, BACTERIAL; DNA, RECOMBINANT; ESCHERICHIA COLI; GENETIC VECTORS; GENOME, BACTERIAL; GENOMICS; MYCOPLASMA GENITALIUM; OLIGODEOXYRIBONUCLEOTIDES; PLASMIDS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MYCOPLASMA GENITALIUM; SACCHAROMYCES CEREVISIAE;

EID: 39449112551     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1151721     Document Type: Article

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33. We thank J. Mulligan for his interest in our work and expediting cassette synthesis by Blue Heron Technologies, S. Vashee and R.-Y. Chuang for many helpful discussions about the manuscript, and J. Johnson and T. Davidsen for assistance with GenBank submissions. Additionally, we thank the Larionov laboratory at NIH for their gifts of yeast strains and TAR cloning expertise. The bulk of the work was supported by Synthetic Genomics, Inc. J.C.V. is Chief Executive Officer and Co-Chief Scientific Officer of Synthetic Genomics, Inc., a privately held entity that develops genomic-driven strategies to address global energy and environmental challenges. H.O.S. is Co-Chief Scientific Officer and on the Board of Directors of Synthetic Genomics, Inc. C.A.H. is Chairman of the Synthetic Genomics, Inc., Scientific Advisory Board. All three of these authors hold Synthetic Genomics, Inc., stock.