Plasmid construction by linker-assisted homologous recombination in yeast

BioTechniques

Volumn 31, Issue 6, 2001, Pages 1246-1250

  Gunyuzlu, P L ^a   Hollis, G F ^a   Toyn, J H ^a  

^a DUPONT COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; DOUBLE STRANDED DNA; GREEN FLUORESCENT PROTEIN; PLASMID VECTOR; RESTRICTION ENDONUCLEASE;

ALLELE; ARTICLE; DNA FLANKING REGION; DNA RECOMBINATION; DNA SEQUENCE; EXPRESSION VECTOR; FLUORESCENCE; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; YEAST;

EID: 0035209031     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01316bm03     Document Type: Article

References (12)

1. The YDp plasmids: A uniform set of vectors bearing versatile gene disruption cassettes for Saccharomyces cerevisiae
2. Oligonucleotide-mediated PCR-independent cloning by homologous recombination
3. Transforming yeast with DNA
4. A yeast genetic assay for caspase cleavage of the amyloidβ precursor protein
5. The complete set of predicted genes from Saccharomyces cerevisiae in a readily usable form
6. Utilization of microhomologous recombination in yeast to generate targeting constructs for mammalian genes
7. Plasmid construction by homologous recombination in yeast
8. Mapping by multifragment cloning in vivo
9. Recombination between similar but not identical DNA sequences during yeast transformation occurs within short stretches of identity
10. Recombination-mediated PCR-directed plasmid construction in vivo in yeast
11. General method for plasmid construction using homologous recombination
12. Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse