One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 51, 2008, Pages 20404-20409

  Gibson, Daniel G ^a   Benders, Gwynedd A ^a   Axelrod, Kevin C ^a   Zaveri, Jayshree ^a   Algire, Mikkel A ^a   Moodie, Monzia ^a   Montague, Michael G ^a   Venter, J Craig ^a   Smith, Hamilton O ^a   Hutchison III, Clyde A ^a  

^a J CRAIG VENTER INSTITUTE   (United States)

Author keywords

Combinatorial assembly; Genome synthesis; In vivo DNA assembly; Mycoplasma genitalium; Synthetic biology; Yeast transformation

Indexed keywords

DNA FRAGMENT;

ARTICLE; BACTERIAL GENOME; CONTROLLED STUDY; DNA DETERMINATION; GENE SYNTHESIS; MYCOPLASMA GENITALIUM; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

CLONING, MOLECULAR; DNA; GENOME, BACTERIAL; MYCOPLASMA GENITALIUM; OLIGODEOXYRIBONUCLEOTIDES; RECOMBINATION, GENETIC; YEASTS;

MYCOPLASMA GENITALIUM; SACCHAROMYCES CEREVISIAE;

EID: 58149510036     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0811011106     Document Type: Article

References (19)

1. Hinnen A, Hicks JB, Fink GR (1978) Transformation of yeast. Proc Natl Acad Sci U S A 75:1929-1933.
2. Orr-Weaver TL, Szostak JW, Rothstein RJ (1981) Yeast transformation: A model system for the study of recombination. Proc Natl Acad Sci U S A 78:6354-6358.
3. Ma H, Kunes S, Schatz PJ, Botstein D (1987) Plasmid construction by homologous recombination in yeast. Gene 58:201-216.
4. Raymond CK, Pownder TA, Sexson SL (1999) General method for plasmid construction using homologous recombination. Biotechniques 26:134-141.
5. Raymond CK, Sims EH, Olson MV (2002) Linker-mediated recombinational subcloning of large DNA fragments using yeast. Genome Res 12:190-197.
6. Marykwas DL, Passmore SE (1995) Mapping by multifragment cloning in vivo. Proc Natl Acad Sci U S A 92:11701-11705.
7. Ebersole T, et al. (2005) Rapid generation of long synthetic tandem repeats and its application for analysis in human artificial chromosome formation. Nucleic Acids Res 33:e130.
8. Gibson DG, et al. (2008) Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Science 319:1215-1220.
9. Kouprina N, Larionov V (2008) Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae. Nat Protoc 3:371-377.
10. Daley JM, Palmbos PL, Wu D, Wilson TE (2005) Nonhomologous end joining in yeast. Annu Rev Genet 39:431-451.
11. Feller W (1957) An Introduction to Probability Theory and Its Applications (Wiley, New York), pp 47-50.
12. Marschall P, Malik N, Larin Z (1999) Transfer of YACs up to 2.3 Mb intact into human cells with polyethylenimine. Gene Ther 6:1634-1637.
13. Bell SP (1995) Eukaryotic replicators and associated protein complexes. Curr Opin Genet Dev 5:162-167.
14. van Brabant AJ, Buchanan CD, Charboneau E, Fangman WL, Brewer BJ (2001) An origin-deficient yeast artificial chromosome triggers a cell cycle checkpoint. Mol Cell 7:705-713.
15. Kouprina N, Eldarov M, Moyzis R, Resnick M, Larionov V (1994) A model system to assess the integrity of mammalian YACs during transformation and propagation in yeast. Genomics 21:7-17.
16. Larionov V, et al. (1994) Transformation-associated recombination between diverged and homologous DNA repeats is induced by strand breaks. Yeast 10:93-104.
17. Holt RA, Warren R, Flibotte S, Missirlis PI, Smailus DE (2007) Rebuilding microbial genomes. Bioessays 29:580-590.
18. Itaya M, Tsuge K, Koizumi M, Fujita K (2005) Combining two genomes in one cell: Stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome. Proc Natl Acad Sci U S A 102:15971-15976.
19. Yonemura I, et al. (2007) Direct cloning of full-length mouse mitochondrial DNA using a Bacillus subtilis genome vector. Gene 391:171-177.