Creation of a bacterial cell controlled by a chemically synthesized genome

Science

Volumn 329, Issue 5987, 2010, Pages 52-56

  Gibson, Daniel G ^a   Glass, John I ^a   Lartigue, Carole ^a   Noskov, Vladimir N ^a   Chuang, Ray Yuan ^a   Algire, Mikkel A ^a   Benders, Gwynedd A ^a   Montague, Michael G ^a   Ma, Li ^a   Moodie, Monzia M ^a   Merryman, Chuck ^a   Vashee, Sanjay ^a   Krishnakumar, Radha ^a   Assad Garcia, Nacyra ^a   Andrews Pfannkoch, Cynthia ^a   Denisova, Evgeniya A ^a   Young, Lei ^a   Qi, Zhi Ng ^a   Segall Shapiro, Thomas H ^a   Calvey, Christopher H ^a   Parmar, Prashanth P ^a   Hutchison III, Clyde A ^a   Smith, Hamilton O ^a   Venter, J Craig ^a   more..

^a J CRAIG VENTER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; PLASMID DNA; SYNTHETIC DNA;

BACTERIUM; CHROMOSOME; FUNCTIONAL MORPHOLOGY; GENETIC ANALYSIS; GENOME; MUTATION; PHENOTYPE; POLYMORPHISM;

ARTICLE; BACTERIAL CELL; BACTERIAL CHROMOSOME; BACTERIAL GENOME; CELL DIVISION; CONTROLLED STUDY; DNA SEQUENCE; GENE DELETION; GENE MUTATION; GENE SEQUENCE; GENE TRANSFER; GENETIC POLYMORPHISM; MYCOPLASMA CAPRICOLUM; MYCOPLASMA MYCOIDES; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; SYNTHESIS;

BACTERIAL PROTEINS; BASE SEQUENCE; BIOENGINEERING; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENE DELETION; GENES, BACTERIAL; GENETIC ENGINEERING; GENOME, BACTERIAL; MOLECULAR SEQUENCE DATA; MYCOPLASMA CAPRICOLUM; MYCOPLASMA MYCOIDES; PHENOTYPE; PLASMIDS; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); MYCOPLASMA MYCOIDES;

EID: 77953584054     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1190719     Document Type: Article

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28. We thank Synthetic Genomics, Inc. for generous funding of this work. We thank J. B. Hostetler, D. Radune, N. B. Fedorova, M. D. Kim, B. J. Szczypinski, I. K. Singh, J. R. Miller, S. Kaushal, R. M. Friedman, and J. Mulligan for their contributions to this work. Electron micrographs were generously provided by T. Deerinck and M. Ellisman of the National Center for Microscopy and Imaging Research at the University of California at San Diego. J.C.V. is chief executive officer and co-chief scientific officer of SGI. H.O.S. is co-chief scientific officer and on the Board of Directors of SGI. C.A.H. is chairman of the SGI Scientific Advisory Board. All three of these authors and JCVI hold SGI stock. JCVI has filed patent applications on some of the techniques described in this paper.