Expression of protein complexes using multiple Escherichia coli protein co-expression systems: A benchmarking study

Journal of Structural Biology

Volumn 175, Issue 2, 2011, Pages 159-170

  Busso, Didier ^a   Peleg, Yoav ^b   Heidebrecht, Tatjana ^c   Romier, Christophe ^a   Jacobovitch, Yossi ^b   Dantes, Ada ^b   Salim, Loubna ^a   Troesch, Edouard ^a   Schuetz, Anja ^d   Heinemann, Udo ^d,e   Folkers, Gert E ^f   Geerlof, Arie ^g,l   Wilmanns, Matthias ^g   Polewacz, Andrea ^h   Quedenau, Claudia ^h   Büssow, Konrad ^h,i   Adamson, Rachel ^j   Blagova, Elena ^j   Walton, Julia ^j   Cartwright, Jared L ^j   Bird, Louise E ^k,m   Owens, Raymond J ^k,m   Berrow, Nick S ^k,n   Wilson, Keith S ^j   Sussman, Joel L ^b   Perrakis, Anastassis ^c   Celie, Patrick H N ^c   more..

^a UNIVERSITÉ DE STRASBOURG   (France)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^c NETHERLANDS CANCER INSTITUTE   (Netherlands)

^d MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^e FREIE UNIVERSITÄT BERLIN   (Germany)

^f UTRECHT UNIVERSITY   (Netherlands)

^g EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^h MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

ⁱ HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^j UNIVERSITY OF YORK   (United Kingdom)

^k UNIVERSITY OF OXFORD   (United Kingdom)

^l INSTITUTE OF EPIDEMIOLOGY   (Germany)

^m RUTHERFORD APPLETON LABORATORY   (United Kingdom)

ⁿ INSTITUTE FOR RESEARCH IN BIOMEDICINE   (Spain)

more..

Author keywords

Cloning strategies; Co expression; Enzyme free cloning; Escherichia coli; Gateway ; In Fusion ; LIC; Restriction free cloning

Indexed keywords

CDT1 GEMININ PROTEIN; ESCHERICHIA COLI PROTEIN; GEMININ; HISTIDINE; IMPORTIN ALPHA 5 PROTEIN; KARYOPHERIN ALPHA; RNA POLYMERASE II; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR IIE ALPHA; TRANSCRIPTION FACTOR IIE BETA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; CELL FUNCTION; CRYSTAL STRUCTURE; ESCHERICHIA COLI; IN VITRO STUDY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; QUALITY CONTROL; STOICHIOMETRY; STRUCTURAL PROTEOMICS;

ACADEMIES AND INSTITUTES; CCAAT-BINDING FACTOR; CELL CYCLE PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI; EUROPE; GENETIC VECTORS; INTERNATIONAL COOPERATION; ISRAEL; MULTIPROTEIN COMPLEXES; RECOMBINANT PROTEINS; TRANSCRIPTION FACTORS, TFII;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 79960022894     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2011.03.004     Document Type: Article

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