Protein complex expression by using multigene baculoviral vectors

Nature Methods

Volumn 3, Issue 12, 2006, Pages 1021-1032

  Fitzgerald, Daniel J ^a   Berger, Philipp ^a,b   Schaffitzel, Christiane ^a   Yamada, Kazuhiro ^a   Richmond, Timothy J ^a   Berger, Imre ^a  

^a ETH ZURICH   (Switzerland)

^b PAUL SCHERRER INSTITUTE   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; HYBRID PROTEIN; MULTIPROTEIN COMPLEX; TRANSPOSASE;

ARTICLE; BACTERIAL CELL; BACULOVIRUS EXPRESSION SYSTEM; CELL CULTURE; COMPLEX FORMATION; CONTROLLED STUDY; DNA RECOMBINATION; ESCHERICHIA COLI; EUKARYOTE; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE CASSETTE; GENE CONSTRUCT; GENE TRANSFER; GENETIC CODE; HOMOLOGOUS RECOMBINATION; IN VITRO STUDY; IN VIVO STUDY; METHODOLOGY; MULTIGENE FAMILY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; VIRUS GENOME; VIRUS VECTOR;

BACULOVIRIDAE; CLONING, MOLECULAR; GENETIC VECTORS; MULTIGENE FAMILY; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSFECTION; VIRAL PROTEINS;

ESCHERICHIA COLI; EUKARYOTA;

EID: 33751247668     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth983     Document Type: Article

References (10)

1. Berger, I., Fitzgerald, D.J. & Richmond, T.J. Baculovirus expression system for heterologous multiprotein complexes. Nat. Biotechnol. 22, 1583-1587 (2004).
2. Benoit, R.M., Wilhelm, R.N., Scherer-Becker, D. & Ostenmeier, C. An improved method for fast, robust, and seamless integration of DNA fragments into multiple plasmids. Protein Expr. Pur. 45, 66-71 (2006).
3. Tan, S., Kern, R.C. & Selleck, W. The pST44 polycistronic expression system for producing protein complexes in E.coli. Protein Expr. Pur. 40, 385-395 (2005).
4. Tolia, N.H. & Joshua-Tor, L. Strategies for protein coexpression in Escherichia coli. Nat. Methods 3, 55-64 (2006).
5. Uetz, P. et al. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature 403, 623-627 (2000).
6. Giot, L. et al. A protein interaction map of Drosophila melanogaster. Science 302, 1727-1736 (2003).
7. Simon, O., Williams, T., Caballero, P. & Lopez-Ferber, M. Dynamics of deletion genotypes in an experimental insect virus population. Proc. R. Soc. Lond. B 273, 783-790 (2006).
8. Pijlman, G.B., de Vrij, J., van den End, F.J., Vlak, J.M. & Martens, D.E. Evaluation of baculovirus expression vectors with enhanced stability in continuous cascaded insect-cell bioreactors. Biotechnol. Bioeng. 87, 743-753 (2004).
9. O'Reilly, D.R., Miller, L.K. & Luckov, V.A. eds. Baculovirus expression vectors. A laboratory manual. (Oxford University Press, New York, 1994).
10. Patterson, G., Day, R.N. & Piston, D. Fluorescent protein spectra. J. Cell Sci. 114, 837-838 (2001).