High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1

PLoS ONE

Volumn 4, Issue 2, 2009, Pages

  Ren, Hui ^a   Yu, Daoyong ^a,b   Ge, Baosheng ^a,b   Cook, Brian ^a   Xu, Zhinan ^a   Zhang, Shuguang ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b CHINA UNIVERSITY OF PETROLEUM   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ARABINOSE; CHEMOKINE RECEPTOR CCR3; CHEMOKINE RECEPTOR CCR5; CHEMOKINE RECEPTOR CX3CR1; CHEMOKINE RECEPTOR CXCR4; DETERGENT; G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN; PLASMID VECTOR;

ARTICLE; BACTERIAL STRAIN; BIOTECHNOLOGICAL PROCEDURES; BIOTECHNOLOGICAL PRODUCTION; CIRCULAR DICHROISM; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; FUSION GENE; GEL PERMEATION CHROMATOGRAPHY; GENE EXPRESSION SYSTEM; IMMUNOBLOTTING; NONHUMAN; POLYMERASE CHAIN REACTION; PROCESS OPTIMIZATION; PROTEIN PURIFICATION; RECEPTOR SOLUBILIZATION;

BACTERIA (MICROORGANISMS); HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 84862531907     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0004509     Document Type: Article

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