The toxicity of recombinant proteins in Escherichia coli: A comparison of overexpression in BL21(DE3), C41(DE3), and C43(DE3)

Protein Expression and Purification

Volumn 37, Issue 1, 2004, Pages 203-206

  Dumon Seignovert, Laurence ^a   Cariot, Guillaume ^a   Vuillard, Laurent ^a  

^a Avidis SA   (France)

Author keywords

[No Author keywords available]

Indexed keywords

RECOMBINANT PROTEIN;

ARTICLE; COMPARATIVE STUDY; ESCHERICHIA COLI; GENETIC TRANSFORMATION; GENETICS; HUMAN; METABOLISM; MICROBIOLOGICAL EXAMINATION; PLASMID;

ESCHERICHIA COLI; HUMANS; MICROBIAL SENSITIVITY TESTS; PLASMIDS; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC;

ESCHERICHIA COLI; VECTORS;

EID: 3543149006     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.04.025     Document Type: Article

References (10)

1. Studier F.W., Rosenberg A.H., Dunn J.J., Dubendorff J.W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:1990;60-89
2. Miroux B., Walker J.E. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 260:1996;289-298
3. Masi M., Pages J.M., Pradel E. Overexpression and purification of the three components of the Enterobacter aerogenes AcrA-AcrB-TolC multidrug efflux pump. J. Chromatogr. B. Anal. Technol. Biomed. Life Sci. 786:2003;197-205
4. Sorensen H.P., Sperling-Petersen H.U., Mortensen K.K. Production of recombinant thermostable proteins expressed in Escherichia coli: completion of protein synthesis is the bottleneck. J. Chromatogr. B. Anal. Technol. Biomed. Life Sci. 786:2003;207-214
5. Voet-van-Vormizeele J., Groth G. High-level expression of the Arabidopsis thaliana ethylene receptor protein ETR1 in Escherichia coli and purification of the recombinant protein. Prot. Expr. Purif. 32:2003;89-94
6. Skogman G., Nilsson J., Gustafsson P. The use of a partition locus to increase stability of tryptophan-operon-bearing plasmids in Escherichia coli. Gene. 23:1983;105-115
7. Miller C.A., Tucker W.T., Meacock P.A., Gustafsson P., Cohen S.N. Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101. Gene. 24:1983;309-315
8. Chambers S.P., Prior S.E., Barstow D.A., Minton N.P. The pMTL nic- cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene. 68:1988;139-149
9. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual, second ed., vol. 3. CSH Laboratory Press, Cold Spring Harbor, NY
10. Arechaga I., Miroux B., Karrasch S., Huijbregts R., de Kruijff B., Runswick M.J., Walker J.E. Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over-production of the b subunit of F(1)F(0) ATP synthase. FEBS Lett. 482:2000;215-219