High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81

PLoS ONE

Volumn 3, Issue 6, 2008, Pages

  Takayama, Hidehito ^a,b   Chelikani, Prashen ^c   Reeves, Philip J ^d   Zhang, Shuguang ^a   Khorana, H Gobind ^c  

^a USA   (United States)

^b MITSUBISHI CHEMICAL CORPORATION   (Japan)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d UNIVERSITY OF ESSEX   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CD81 ANTIGEN; DNA; GLYCOPROTEIN E2; MEMBRANE PROTEIN; MEMBRANE RECEPTOR; TETRACYCLINE; TETRASPANIN; LEUKOCYTE ANTIGEN; RECOMBINANT PROTEIN;

ANTIBODY AFFINITY; ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ANTIGEN STRUCTURE; ARTICLE; CELL MEMBRANE; CELL STRAIN HEK293; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE ANALYSIS; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN FAMILY; PROTEIN LOCALIZATION; AFFINITY CHROMATOGRAPHY; CELL FRACTIONATION; CELL LINE; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; GLYCOSYLATION; IMMUNOLOGY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION;

HEPATITIS C VIRUS; MAMMALIA;

ANTIGENS, CD; CELL LINE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GLYCOSYLATION; HUMANS; MODELS, MOLECULAR; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBCELLULAR FRACTIONS;

EID: 48449095093     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0002314     Document Type: Article

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