High-throughput enzyme evolution in Saccharomyces cerevisiae using a synthetic RNA switch

Metabolic Engineering

Volumn 14, Issue 4, 2012, Pages 306-316

  Michener, Joshua K ^a   Smolke, Christina D ^b  

^a California Institute of Technology   (United States)

^b Stanford University   (United States)

Author keywords

High throughput screen; P450; RNA switch; Synthetic biology

Indexed keywords

APTAMERS; DEMETHYLASE; DIRECTED EVOLUTION; ENZYME LIBRARIES; FINE CHEMICALS; FLUORESCENCE ACTIVATED CELL SORTING; GFP EXPRESSION; HIGH-THROUGHPUT; IN-VIVO; LIVING CELL; METABOLIC PATHWAYS; METABOLITE CONCENTRATIONS; P450; PRODUCT SELECTIVITIES; RENEWABLE RESOURCE; SCREENING TECHNIQUES; SINGLE CELLS; SMALL MOLECULES; SYNTHETIC BIOLOGY; WHOLE CELL;

BIOSENSORS; CELL CULTURE; CLONE CELLS; FLUORESCENCE; LIBRARIES; RNA; YEAST;

ENZYMES;

APTAMER; GREEN FLUORESCENT PROTEIN; RNA;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYMIC BIOSENSOR; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HIGH THROUGHPUT SCREENING; IN VIVO STUDY; METABOLIC ENGINEERING; MOLECULAR EVOLUTION; MOLECULAR LIBRARY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RIBOSWITCH; SACCHAROMYCES CEREVISIAE;

CYTOCHROME P-450 CYP1A2; DIRECTED MOLECULAR EVOLUTION; ENZYMES; FLOW CYTOMETRY; HIGH-THROUGHPUT SCREENING ASSAYS; METABOLIC ENGINEERING; MUTATION; RIBOSWITCH; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 84862167101     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2012.04.004     Document Type: Article

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