Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases

Journal of the American Chemical Society

Volumn 132, Issue 30, 2010, Pages 10570-10577

  Yang, Guangyu ^a,b,c   Rich, Jamie R ^a   Gilbert, Michel ^d   Wakarchuk, Warren W ^d   Feng, Yan ^b,c   Withers, Stephen G ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b SHANGHAI JIAO TONG UNIVERSITY   (China)

^c JILIN UNIVERSITY   (China)

^d NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BROAD APPLICATION; BUILDING BLOCKES; DIRECTED EVOLUTION; EFFICIENT SYNTHESIS; FLUORESCENCE ACTIVATED CELL SORTING; GALACTOSYLTRANSFERASE; GLYCOSPHINGOLIPIDS; GLYCOSYLTRANSFERASES; HIGH-THROUGHPUT SCREENING; NEUTRAL SUGAR; PROBABILITY OF FALSE POSITIVES; PROTEIN ENGINEERING; SCREENING PROTOCOL; SIALYLTRANSFERASES; SUBSTRATE SPECIFICITY; TWO-COLOR; ULTRA-HIGH; WILD-TYPE ENZYMES;

BIOCHEMICAL ENGINEERING; BIOLOGY; FLUORESCENCE; GENETIC ENGINEERING; OLIGOSACCHARIDES; SUGAR (SUCROSE); THROUGHPUT;

ENZYMES;

GLYCOSPHINGOLIPID; GLYCOSYLTRANSFERASE; OLIGOSACCHARIDE;

ARTICLE; ENZYME ACTIVITY; ENZYME SUBSTRATE; FALSE POSITIVE RESULT; FLUORESCENCE ACTIVATED CELL SORTING; HIGH THROUGHPUT SCREENING; MUTANT; PROBABILITY; SYNTHESIS; WILD TYPE;

CAMPYLOBACTER JEJUNI; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE EXPRESSION; GLYCOSYLTRANSFERASES; HIGH-THROUGHPUT SCREENING ASSAYS; MUTATION;

EID: 77955042704     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja104167y     Document Type: Article

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