Interrogation of global active site occupancy of a fungal iterative polyketide synthase reveals strategies for maintaining biosynthetic fidelity

Journal of the American Chemical Society

Volumn 134, Issue 15, 2012, Pages 6865-6877

  Vagstad, Anna L ^a   Bumpus, Stefanie B ^b   Belecki, Katherine ^a   Kelleher, Neil L ^c   Townsend, Craig A ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c NORTHWESTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; AROMATIC NATURAL PRODUCTS; ASPERGILLUS PARASITICUS; BIOLOGICAL PROPERTIES; BIOSYNTHETIC PATHWAY; CATALYTIC DOMAINS; CHAIN EXTENSION; COVALENTLY BOUND; EXTENSION UNITS; FOURIER TRANSFORM MASS SPECTROMETRY; IN-VITRO; NORSOLORINIC ACID; POLYKETIDE ASSEMBLY; POLYKETIDE SYNTHASES; POLYKETIDES; PROCESSIVITY; PRODUCT FORMATION; PRODUCT RELEASE; RELATIVE RATES; SIMULTANEOUS OBSERVATION; SMALL MOLECULES; SUBSTRATE LOADING; THIOESTERASES;

ASPERGILLUS; BIOSYNTHESIS; CATALYSIS; CYCLIZATION; KETONES; MASS SPECTROMETRY; PLANTS (BOTANY); REACTION INTERMEDIATES; SUBSTRATES;

LOADING;

AFLATOXIN; FUNGAL ENZYME; POLYKETIDE SYNTHASE; THIOL ESTER HYDROLASE;

ARTICLE; ASPERGILLUS PARASITICUS; BIOSYNTHESIS; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; COVALENT BOND; ENZYME ACTIVE SITE; ENZYME SUBSTRATE; HYDROLYSIS; IN VITRO STUDY; NONHUMAN; PROTEIN DOMAIN;

BIOCATALYSIS; CATALYTIC DOMAIN; FUNGAL PROTEINS; KINETICS; POLYKETIDE SYNTHASES;

EID: 84860015588     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja3016389     Document Type: Article

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