Deconstruction of iterative multidomain polyketide synthase function

Science

Volumn 320, Issue 5873, 2008, Pages 243-246

  Crawford, Jason M ^a,c   Thomas, Paul M ^b   Scheerer, Jonathan R ^a   Vagstad, Anna L ^a   Kelleher, Neil L ^b   Townsend, Craig A ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTINORHODINE; KETOSYNTHASE; POLYKETIDE SYNTHASE; TETRACYCLINE; THIOL ESTER HYDROLASE; UNCLASSIFIED DRUG;

CARCINOGEN; CATALYSIS; ENZYME;

ARTICLE; CATALYSIS; CYCLIZATION; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME RECONSTITUTION; NONHUMAN; PRIORITY JOURNAL;

ACYL CARRIER PROTEIN; AFLATOXIN B1; ALGORITHMS; ANTHRAQUINONES; ASPERGILLUS; CATALYTIC DOMAIN; COMPUTATIONAL BIOLOGY; CYCLIZATION; MASS SPECTROMETRY; OXIDATION-REDUCTION; POLYKETIDE SYNTHASES; PROTEIN STRUCTURE, TERTIARY;

EID: 42049099494     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1154711     Document Type: Article

References (15)

1. J. Staunton, K. J. Weissman, Nat. Prod. Rep. 18, 380 (2001).
2. M. A. Fischbach, C. T. Walsh, Chem. Rev. 106, 3468 (2006).
3. J. M. Crawford, A. L. Vagstad, K. C. Ehrlich, C. A. Townsend, Bioorg. Chem. 36, 16 (2008).
4. D. Udwary, M. Merski, C. A. Townsend, J. Mol. Biol. 323, 585 (2002).
5. J. M. Crawford, B. C. R. Dancy, E. A. Hill, D. W. Udwary, C. A. Townsend, Proc. Natl. Acad. Sci. U.S.A. 103, 16728 (2006).
6. C. Sanchez, L. Du, D. J. Edwards, M. D. Toney, B. Shen, Chem. Biol. 8, 725 (2001).
7. I. Fujii, A. Watanabe, U. Sankawa, Y. Ebizuka, Chem. Biol. 8, 189 (2001).
8. Z.-G. Chen, I. Fujii, Y. Ebizuka, U. Sankawa, Phytochem 38, 299 (1995).
9. R. McDaniel, S. Ebert-Khosla, D. A. Hopwood, C. Khosla, Science 262, 1546 (1993).
10. Y. Tang, S. C. Tsai, C. Khosla, J. Am. Chem. Soc. 125, 12708 (2003).
11. A. T. Keatinge-Clay, D. A. Maltby, K. F. Medzihradszky, C. Khosla, R. M. Stroud, Nat. Struct. Mol. Biol. 11, 888 (2004).
12. Y. Ma et al., ChemBioChem 7, 1951 (2006).
13. S. M. Ma et al., J. Am. Chem. Soc. 130, 38 (2008).
14. P. C. Dorrestein et al., Biochemistry 45, 12756 (2006).
15. Supported by NIH research grants ES001670 (C.A.T.) and GM067725 (N.L.K.), NIH Chemical Biology Interface training grant GM070421 (P.M.T.), and NIH postdoctoral fellowship GM079408 (J.R.S.).