Enhanced antibody production following intermediate addition based on flux analysis in mammalian cell continuous culture

Bioprocess and Biosystems Engineering

Volumn 33, Issue 1, 2010, Pages 117-125

  Omasa, Takeshi ^a   Furuichi, Keisuke ^a,c   Iemura, Tomoya ^a,d   Katakura, Yoshio ^a   Kishimoto, Michimasa ^b   Suga, Ken Ichi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^c MEIJI DAIRIES CORPORATION   (Japan)

^d KIRIN BREWERY CO LTD   (Japan)

Author keywords

Antibody production; Energy metabolism; Flux; Mammalian cell; Metabolic engineering

Indexed keywords

ANTIBODY PRODUCTION; ATP PRODUCTION RATES; CELL VOLUME; CONTINUOUS CULTURE; DILUTION RATE; DRY CELLS; ENERGY METABOLISM; ENERGY SOURCE; FLUX ANALYSIS; MAMMALIAN CELLS; METABOLIC ENGINEERING; METABOLIC FLUX; METABOLIC PATHWAYS; PRIMARY ENERGY SOURCE; PRODUCTION RATES; PYRUVATES; RIBONUCLEASE A; SPECIFIC GROWTH RATE; STEADY-STATE CONDITION;

AMINO ACIDS; ANIMAL CELL CULTURE; ANTIBODIES; CELL CULTURE; CELLS; GLUCOSE; MAMMALS; METABOLISM;

CONTINUOUS CELL CULTURE;

ADENOSINE TRIPHOSPHATE; ANTIBODY; CITRIC ACID; GLUCOSE; GLUTAMINE; MALIC ACID; PYRUVIC ACID; RIBONUCLEASE A ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL LINE; CELL METABOLISM; CELL VOLUME; CONTINUOUS CULTURE; DILUTION; DRY CELL WEIGHT; ENERGY METABOLISM; HYBRIDOMA; MAMMAL CELL; METABOLIC ENGINEERING; MOUSE; NONHUMAN; PARAMETER; PRIORITY JOURNAL; STEADY STATE;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CULTURE MEDIA, SERUM-FREE; HYBRIDOMAS; MICE; MODELS, BIOLOGICAL;

MAMMALIA;

EID: 76849087459     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-009-0351-8     Document Type: Article

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