A gene optimization strategy that enhances production of fully functional P-Glycoprotein in Pichia pastoris

PLoS ONE

Volumn 6, Issue 8, 2011, Pages

  Bai, Jiangping ^a,e   Swartz, Douglas J ^a   Protasevich, Irina I ^b   Brouillette, Christie G ^b   Harrell, Patina M ^a   Hildebrandt, Ellen ^a   Gasser, Brigitte ^c   Mattanovich, Diethard ^c   Ward, Andrew ^d   Chang, Geoffrey ^d   Urbatsch, Ina L ^a  

^a TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^d SCRIPPS RESEARCH INSTITUTE   (United States)

^e GANSU AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CYCLOSPORIN A; GLYCOPROTEIN P; VERAPAMIL; MEMBRANE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CANCER RESISTANCE; CODON; CONTROLLED STUDY; ENTHALPY; ENZYME ACTIVITY; MULTIDRUG RESISTANCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PROCESS OPTIMIZATION; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN HYDROLYSIS; PROTEIN METABOLISM; PROTEIN PROCESSING; PROTEIN QUATERNARY STRUCTURE; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; STRUCTURE ANALYSIS; THERMOSTABILITY; CHEMISTRY; GENETICS; METABOLISM; PICHIA; SACCHAROMYCES CEREVISIAE;

MAMMALIA; PICHIA PASTORIS;

CODON; MEMBRANE PROTEINS; P-GLYCOPROTEIN; PICHIA; SACCHAROMYCES CEREVISIAE;

EID: 79961081813     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0022577     Document Type: Article

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