Large scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild- type, Walker A, and Walker B mutant proteins

Journal of Biological Chemistry

Volumn 274, Issue 49, 1999, Pages 34711-34718

  Lerner Marmarosh, Nicole ^a   Gimi, Khursheed ^a   Urbatsch, Ina L ^a   Gros, Philippe ^b   Senior, Alan E ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^b MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE DERIVATIVE; AZOLECTIN; DITHIOTHREITOL; GLYCOPROTEIN P; LIPID; MAGNESIUM; MUTANT PROTEIN;

ARTICLE; BINDING AFFINITY; CATALYSIS; CONTROLLED STUDY; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; CHROMATOGRAPHY; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; FLUORESCENT DYES; KINETICS; MICE; MICROSOMES; MUTAGENESIS; P-GLYCOPROTEINS; PICHIA; PROTEIN BINDING; SPECTROMETRY, FLUORESCENCE; TRYPTOPHAN;

EID: 0033520934     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.274.49.34711     Document Type: Article

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