High level expression of human endostatin in Pichia pastoris using a synthetic gene construct

Applied Microbiology and Biotechnology

Volumn 73, Issue 6, 2007, Pages 1355-1362

  Su, Zhijian ^a,b   Wu, Xiaoping ^b   Feng, Ya ^b   Ding, Changcai ^b   Xiao, Yechen ^c   Cai, Lu ^d   Feng, Wenke ^d   Li, Xiaokun ^a,b,c  

^a WENZHOU MEDICAL UNIVERSITY   (China)

^b JINAN UNIVERSITY   (China)

^c JILIN AGRICULTURAL UNIVERSITY   (China)

^d University of Louisville School of Medicine   (United States)

Author keywords

Artificial synthetic gene; Endostatin; High density fermentation; Pichia pastoris

Indexed keywords

BIOASSAY; CELL CULTURE; COLLAGEN; GENES; TUMORS; YEAST;

ARTIFICIAL SYNTHETIC GENE; ENDOSTATIN; FIBROBLAST GROWTH FACTOR; HIGH-DENSITY FERMENTATION; RECOMBINANT PROTEIN;

GENETIC ENGINEERING;

ACIDIC FIBROBLAST GROWTH FACTOR; COLLAGEN TYPE 18; ENDOSTATIN; HEPARIN; RECOMBINANT PROTEIN; SYNTHETIC DNA;

COLLAGEN; FERMENTATION; GENE; GENE EXPRESSION; INHIBITOR; RECOMBINATION; YEAST;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BIOREACTOR; CELL PROLIFERATION; CLINICAL PRACTICE; CODON; CONTROLLED STUDY; CULTURE MEDIUM; ENDOTHELIUM CELL; FERMENTATION TECHNIQUE; GENE CONSTRUCT; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; PURIFICATION; SPECIES CULTIVATION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CELL PROLIFERATION; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOSTATINS; ENDOTHELIAL CELLS; GENES, SYNTHETIC; HUMANS; MICE; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 33846147716     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0604-2     Document Type: Article

References (15)

1. Bertolini F, Fusetti L, Mancuso P, Gobbi A, Corsini C, Ferrucci PF, Martinelli G, Pruneri G (2000) Endostatin, an antiangiogenic drug, induces tumor stabilization after chemotherapy or anti-CD20 therapy in a NOD/SCID mouse model of human high-grade non-Hodgkin lymphoma. Blood 96:282-287
2. Boehm T, Folkman J, Browder T, O'Reilly MS (1997) Antiangiogenic therapy of experimental cancer does not induce acquired drug resistance. Nature 390:404-407
3. Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24:45-66
4. Dhanabal M, Ramchandran R, Volk R, Stillman IE, Lombardo M, Iruela-Arispe ML, Simons M, Sukhatme VP (1999a) Endostatin, yeast production, mutants, and antitumor effect in renal cell carcinoma. Cancer Res 59:189-197
5. Dhanabal M, Volk R, Ramchandran R, Simons M, Sukhatme VP (1999b) Cloning, expression, and in vitro activity of human endostatin. Biochem Biophys Res Commun 258:345-352
6. Feng W, Cai J, Pierce WM, Song ZH (2002) Expression of CB2 cannabinoid receptor in Pichia pastoris. Protein Expr Purif 26:496-505
7. Folkman J (1995) Seminars in Medicine of the Beth Israel Hospital, Boston. Clinical applications of research on angiogenesis. N Engl J Med 333:1757-1763
8. Herbst RS, Mullani ZA, Davis DW, Hess KR, McConkey DJ, Charnsangavej C, O'Reilly MS, Kim HW, Baker C, Roach J, Ellis ML, Rashid A, Pluda J, Bucana C, Madden TL, Tran HT, Abbruzzese JL (2003) Development of biologic markers of response and assessment of antiangiogenic activity in a clinical trial of human recombinant endostatin. J Clin Oncol 20:3804-3814
9. Huang Y, Su Z, Zheng Q (2003) Human TGF-β3 gene synthesis and expression in E. coli. Journal of China Pharmaceutical University 34:264-267
10. Gellissen G (2000) Heterologous protein production in methylotrophic yeasts. Appl Microbiol Biotechnol 54:741-750
11. Kunes YZ, Sanz MC, Tumanova I, Birr CA, Shi PQ, Bruguera P, Ruiz JA, Sanchez-Martinez D (2002) Expression and characterization of a synthetic protein C activator in Pichia pastoris. Protein Expr Purif 26:406-415
12. Mundhenke C, Thomas JP, Wilding G, Lee FT, Kelzc F, Chappell R, Neider R, Sebree LA, Friedl A (2003) Tissue examination to monitor antiangiogenic therapy: a phase I clinical trial with endostatin. Clin Cancer Res 7:3366-3374
13. O'Reilly MS, Boehm T, Shing Y, Fukai N, Vasios G, Flynn E, Birkhead JR, Olsen BR (1997) Endostatin: an endogenous inhibitor of angiogenesis and tumor growth. Cell 88:277-285
14. Sinclair G, Choy FY (2002) Synonymous codon usage bias and the expression of human glucocerebrosidase in the methylotrophic yeast, Pichia pastoris. Protein Expr Purif 26:96-105
15. Thomas JP, Arzoomanian RZ, Alberti D, Marnocha R, Lee F, Friedl A, Tutsch K, Dresen A, Geiger P, Pluda J, Fogler W, Schiller JH, Wilding G (2003) Phase I pharmacokinetic and pharmacodynamic study of recombinant human endostatin in patients with advanced solid tumors. J Clin Oncol 20:223-231