Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris

Gene

Volumn 190, Issue 1, 1997, Pages 55-62

  Sreekrishna, Koti ^a   Brankamp, Robert G ^a   Kropp, Keith E ^a   Blankenship, Dale T ^a   Tsay, Jiu Tsair ^a   Smith, Phil L ^a   Wierschke, Jonathan D ^a   Subramaniam, Arun ^a   Birkenberger, Lori A ^a  

^a SANOFI   (France)

Author keywords

5' Untranslated sequence; Alcohol oxidase promoter; Apoptosis; Cell shrinkage; Clonal variation; Expression plasmid design; Fermentation; G418(R) selection; Gene dosage; Growth and media optimization; Integrative transformation; MRNA secondary structure; Protease deficient strain; Secretion; Synthetic gene; Transcriptional barrier

Indexed keywords

ALCOHOL OXIDASE; COMPLEMENTARY DNA; FUNGAL PROTEIN; MATRIX METALLOPROTEINASE; MESSENGER RNA; PROTEINASE; TISSUE INHIBITOR OF METALLOPROTEINASE;

CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; ENDOMYCETALES; ENZYME DEGRADATION; FUNGUS GROWTH; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SECRETION; PROTEIN SYNTHESIS; RNA TRANSLATION; START CODON;

CLONING, MOLECULAR; GENE DOSAGE; GLYCOPROTEINS; HUMANS; METALLOENDOPEPTIDASES; METHANOL; PHENOTYPE; PICHIA; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; TISSUE INHIBITOR OF METALLOPROTEINASES;

EID: 0030997654     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00672-5     Document Type: Conference Paper

References (40)

1. Barr, K.A., Hopkins, S.A. and Sreekrishna, K. (1992) Protocol for efficient secretion of HSA developed from Pichia pastoris. Pharm. Eng. 12, 48-51.
2. Boudreau, N., Sympson, C.J., Werb, Z. and Bissell, M.J. (1995) Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix. Science 267, 891-893.
3. Brankamp, R.G., Sreekrishna, K., Smith, P.L., Blankenship, D.T. and Cardin, A.D. (1995) Expression of a synthetic gene encoding the anticoagulant-antimetastatic protein ghilanten by the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 6, 813-820.
4. Brierley, R.A., Davis, G.R. and Holtz, G.C. (1994) Production of insulin-like growth factor 1 in methylotrophic yeast cells, United States Patent, No. 5,324,639.
5. Chambers, R.S., Broughton, M.J., Cannon, R.D., Carne, A., Emerson, G.W. and Sullivan, P.A. (1993) An exo-β-(1,3)-glucanase of Candida albicans: purification of the enzyme and molecular cloning of the gene. J. Gen. Microbiol. 139, 325-334.
6. Chen, Y., Pioli, D. and Piper, P.W. (1994) Overexpression of the gene for polyubiquitin in yeast confers increased secretion of a human leukocyte protease inhibitor. Bio/Technology 12, 819-823.
7. Clare, J.J., Rayment, F.B., Ballantine, S.P., Sreekrishna, K. and Romanos, M.A. (1991a) High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integration of the gene. Bio/Technology 9, 455-460.
8. Clare, J.J., Romanos, M.A., Rayment, F.B., Rowedder, J.E., Smith, M.A., Payne, M.M., Sreekrishna, K. and Henwood, C.A. (1991b) Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105, 205-212.
9. Cregg, J.M., Barringer, K.J., Hessler, A.Y. and Madden, K.R. (1985) Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5, 3376-3385.
10. Cregg, J.M., Tschopp, J.F., Stilman, C., Siegel, R., Akong, M., Craig, W.S., Buckholz, R.G., Madden, K.R., Kellaris, P.A., Davis, G.R., Smiley, B.L., Cruze, J., Toregrossa, R., Velicelebi, G. and Thill, G.P. (1987) High-level expression and efficient assembly of hepatitis B surface antigen in methylotrophic yeast, Pichia pastoris. Bio/Technology 5, 479-485.
11. Digan, M.E., Lair, S.V., Brierly, R.A., Siegel, R.S., Williams, M.E., Ellis, S.B., Kellaris, P.A., Provow, S.A., Craig, W.S., Velicelebi, G.V., Harpold, M.M. and Thill G.P. (1989) Continuous production of a novel lysozyme via secretion from the yeast, Pichia pastoris. Bio/Technology 7, 160-164.
12. Laroche, Y., Storme, V., De Meutter, J., Messens, J. and Lauwereys, M. (1994) High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris. Bio/Tecnology 12, 1119-1124.
13. Matrisian, L.M. (1990) Metalloproteinases and their inhibitors in matrix remodelling. Trends Genet. 6, 101-105.
14. Muthukumar, G., Suhng, S.H., Magee, P.T., Jewell, R.D. and Primerano, D.A. (1993) The Saccharomyces cerevisiae SPR1 gene encodes a sporulation specific exo-1,3-β-glucanase which contributes to ascospore thermoresistance. J. Bacteriol. 175, 386-394.
15. Ridder, R., Schmitz, R., Legay, Y.F. and Gram, H. (1995) Generation of rabbit monoclonal antibody fragment from combinatorial phage display library and their production in the yeast Pichia pastoris. Bio/Technology 13, 255-260.
16. Romanos, M.A., Clare, J.J., Beesley, K.M., Rayment, F.B., Ballantine, S.P., Makoff, A.J., Dougan, G., Fairweather, N.F. and Charles, L.G. (1991) Recombinant Bordetella pertussis (P69) from the yeast Pichia pastoris: High-level production and immunological properties. Vaccine 9, 901-906.
17. Romanos, M.A., Scorer, C.A. and Clare, J.J. (1992) Foreign gene expression in yeast: a review. Yeast 8, 423-488.
18. Scorer, C.A., Buckholz, R.G., Clare, J.J. and Romanos, M.A. (1993) The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene 136, 111-119.
19. Scorer, C.A., Clare, J.J., McCombie, W.R., Romanos, M.A. and Sreekrishna, K. (1994) Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Bio/Technology 12, 181-184.
20. Siegel, R.S., Buckholz, R.G., Thill, G.P. and Wondrack, L.M. (1990) Production of epidermal growth factor in methylotrophic yeasts. International Patent Application, Publ. No. WO 90/10697.
21. Sreekrishna, K. (1993) Strategies for optimizing protein expression and secretion in the methylotrophic yeast Pichia pastoris. In: Baltz, R.H., Hegeman, G.D. and Skatrud, P.L. (Eds.), Industrial Microorganisms: Basic and Applied Molecular Genetics. American Society of Microbiology, Washington, DC, pp. 119-126.
22. Sreekrishna, K. and Dickson, R.C. (1985) Construction of strains of Saccharomyces cerevisiae that grow on lactose. Proc. Natl. Acad. Sci. USA 82, 7909-7913.
23. Sreekrishna, K. and Kropp, K. (1996) Pichia pastoris. In: Wolf, K. (Ed.), Non-Conventional Yeasts in Biotechnology. Springer, Berlin, pp. 203-252.
24. Sreekrishna, K., Webster, T.D. and Dickson, R.C. (1984) Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903. Gene 28, 73-81.
25. Sreekrishna, K., Tschopp, J.F. and Fuke, M. (1987) Invertase gene (SUC2) of Saccharomyces cerevisiae as a dominant marker for transformation of Pichia pastoris. Gene 59, 115-125.
26. Sreekrishna, K., McCombie, W.R., Potenz, R., Parker, K.A., Mazzaferro, P.K., Maine, G.M., Lopez, J.L., Divelbiss, D.K., Holden, K.A., Barr, R.D. and Fuke, M. (1988a) Clonal variation in the expression of human tumor necrosis factor (TNF) in the methylotrophic yeast Pichia pastoris. Poster presentation at the Fifth Annual Biotech USA Industry Conference and Exhibition, San Francisco, CA, 1988.
27. Sreekrishna, K., Potenz, R.H., Cruze, J.A., McCombie, W.R., Parker, K.A., Nelles, L., Mazzaferro, P.K., Holden, K.A., Harrison, R.G., Wood, P.J., Phelps, D.A., Hubbard, C.E. and Fuke, M. (1988b) High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris. J. Basic Microbiol. 28, 265-278.
28. Sreekrishna, K., Nelles, L., Potenz, R., Cruze, J., Mazzaferro, P., Fish, W., Fuke, M., Holden, K., Phelps, D., Wood, P. and Parker, K. (1989) High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris. Biochemistry 28, 4117-4125.
29. Sreekrishna, K., Barr, K.A., Hoard, S.A., Prevatt, W.D., Torregrosa, R.E., Levingston, R.E., Cruze, J.A. and Wegner, G.H. (1990) Expression of human serum albumin in Pichia pastoris. Topic 09-37B in: Oliver, S.G. and Wickner, R. (Eds.), 15th Int. Congr. Yeast Genetics and Molecular Biology, 1990, The Hague, The Netherlands. Yeast 6 (Special Issue). Wiley, New York, NY, p. S447.
30. Sreekrishna, K., Prevatt, W.D., Thill, G.P., Davis, G.R., Koutz, P., Barr, K.A. and Hopkins, S.A. (1993) Production of Bacillus entomotoxins in methylotrophic yeast. European Patent Application, Publ. No. EP0586 892 A1.
31. Sreekrishna, K., Blankenship, D.T., Brankamp, R.G. and Smith, P.L. (1995) Discovery of free ubiquitin in the culture medium of the methylotrophic yeast Pichia pastoris. ASBMB/DBC-ACS Joint Meeting, 21-25 May 1995. FASEB J. 9, LB84 (Abstract).
32. Thill, G.P., Davis, G.R., Stillman, G.R., Holtz, C., Brierly, R., Engel, M., Buckholz, R., Kinney, J., Provo, S., Vedvick, T. and Siegel, R.S. (1990) Positive and negative effects of multi-copy integrated expression vectors on protein expression in Pichia pastoris. In: Heslot, H., Davies, J., Florent, J., Bobichon, L., Durand, G. and Paris, P.L. (Eds.), Proceedings of the Sixth International Symposium on Genetics of Industrial Microorganisms, Strasbourg, France, 1990. Société Française de Microbiologie, Paris, pp. 477-490.
33. Tschopp, J.F., Svelow, G., Kosson, R., Craig, W. and Grinna, L. (1987) High-level secretion of glycosylated invertase in the methylotrophic yeast, Pichia pastoris. Bio/Technology 5, 1305-1308.
34. Van Nostrand, W.E., Schmaier, A.H., Neiditch, B.R., Siegel, R.S., Raschke, W.C., Sisodia, S.S. and Eagner, S.L. (1994) Expression, purification and characterization of the Kunitz type proteinase inhibitor domain of the amyloid β-protein precursor like protein-2. Biochim. Biophys. Acta 1209, 165-170.
35. Wagner, S.L., Siegel, R.S., Vedvick, T.S., Raschke, W.C. and Van Nostrand, W.E. (1992) High-level expression, purification and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid β-protein precursor. Biochem. Biophys. Res. Commun. 186, 1138-1145.
36. Wegner, G.H. (1983) Biochemical conversions by yeast fermentation at high cell densities. U.S. Patent 4,414,329.
37. Wegner, G.H. (1990) Emerging applications of the methylotrophic yeasts. FEMS Microbiol. Rev. 87, 279-284.
38. 1 compounds. In: Van Verseveld, H.W. and Duine, J.A (Eds.), Proceedings of the 5th International Symposium. Martinus Nijhoff, Dordrecht, pp. 139-149.
39. White, C.E., Kempi, N.M. and Komives, E.A. (1994) Expression of highly disulfide bonded proteins in Pichia pastoris. Structure 2, 1003-1005.
40. Wilhelm, S.M., Collier, I.E., Marmer, B.L., Eisen, A.Z., Grant, G.A. and Goldberg, G.I. (1989) SV40 transformed human lung fibroblasts secrete a 92 kDa type IV collagenase which is identical to that secreted by normal human macrophages. J. Biol. Chem. 264, 17213-17221.