-
1
-
-
0030272217
-
Quality and authenticity of heterologous proteins synthesized in yeast
-
Eckart MR, Bussineau CM Quality and authenticity of heterologous proteins synthesized in yeast. Curr Opin Biotechnol. 7:1996;525-530.
-
(1996)
Curr Opin Biotechnol
, vol.7
, pp. 525-530
-
-
Eckart, M.R.1
Bussineau, C.M.2
-
2
-
-
0002283070
-
Expression in the methylotrophic yeast Pichia pastoris
-
J.M. Fernandez, & J.P. Hoeffler. San Diego, CA: Academic Press. A good introduction to the P. pastoris and other expression systems. The advantages and disadvantages for the production of a specific class of proteins are described.
-
Cregg JM Expression in the methylotrophic yeast Pichia pastoris. Fernandez JM, Hoeffler JP Gene Expression Systems: Using Nature for the Art of Expression. 1999;157-191 Academic Press, San Diego, CA. A good introduction to the P. pastoris and other expression systems. The advantages and disadvantages for the production of a specific class of proteins are described.
-
(1999)
Gene Expression Systems: Using Nature for the Art of Expression
, pp. 157-191
-
-
Cregg, J.M.1
-
3
-
-
0001078966
-
Production of foreign proteins in the yeast Pichia pastoris
-
Cregg JM, Higgins DR Production of foreign proteins in the yeast Pichia pastoris. Can J Bot. 73(suppl 1):1995;891-897.
-
(1995)
Can J Bot
, vol.73
, Issue.SUPPL. 1
, pp. 891-897
-
-
Cregg, J.M.1
Higgins, D.R.2
-
4
-
-
0026778048
-
Foreign gene expression in yeast: A review
-
Romanos MA, Scorer CA, Clare JJ Foreign gene expression in yeast: a review. Yeast. 8:1992;423-488.
-
(1992)
Yeast
, vol.8
, pp. 423-488
-
-
Romanos, M.A.1
Scorer, C.A.2
Clare, J.J.3
-
5
-
-
0028801044
-
Advances in the use of Pichia pastoris for high-level gene expression
-
Romanos R Advances in the use of Pichia pastoris for high-level gene expression. Curr Opin Biotechnol. 6:1995;527-533.
-
(1995)
Curr Opin Biotechnol
, vol.6
, pp. 527-533
-
-
Romanos, R.1
-
7
-
-
0031657285
-
Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Y. lipolytica
-
Muller S, Sandal S, Kamp-Hansen P, Dalboge H Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Y. lipolytica. Yeast. 14:1998;1267-1283.
-
(1998)
Yeast
, vol.14
, pp. 1267-1283
-
-
Muller, S.1
Sandal, S.2
Kamp-Hansen, P.3
Dalboge, H.4
-
8
-
-
0031692690
-
Two novel gene expression systems based on the yeasts Schwanniomyces occidentalis and Pichia stipitis
-
Piontek M, Hagedorn J, Hollenberg CP, Gellissen G, Strasser AWM Two novel gene expression systems based on the yeasts Schwanniomyces occidentalis and Pichia stipitis. Appl Microbiol Biotechnol. 50:1998;331-338.
-
(1998)
Appl Microbiol Biotechnol
, vol.50
, pp. 331-338
-
-
Piontek, M.1
Hagedorn, J.2
Hollenberg, C.P.3
Gellissen, G.4
Strasser, A.W.M.5
-
9
-
-
0031010051
-
Application of yeasts in gene expression studies: A comparison of Saccharomyces cerevisiae, Hansenula polymorpha, and Kluyveromyces lactis - A review
-
Gellisen G, Hollenberg C Application of yeasts in gene expression studies: a comparison of Saccharomyces cerevisiae, Hansenula polymorpha, and Kluyveromyces lactis - a review. Gene. 190:1997;87-97.
-
(1997)
Gene
, vol.190
, pp. 87-97
-
-
Gellisen, G.1
Hollenberg, C.2
-
10
-
-
0344507992
-
-
D.R. Higgins, & J.M. Cregg. Totowa, NJ: Humana Press
-
Higgins DR, Cregg JM Pichia Protocols, vol 103. 1998;Humana Press, Totowa, NJ.
-
(1998)
Pichia Protocols, Vol 103
-
-
-
12
-
-
85030367944
-
Expression of heterologous proteins in the methylotrophic yeast Pichia pastoris
-
in press. This review catalogs the heterologous proteins that have been produced in P. pastoris over the past fifteen years, including both intracellular and extracellular proteins.
-
Lin Cereghino J, Cregg JM Expression of heterologous proteins in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 1999;. in press. This review catalogs the heterologous proteins that have been produced in P. pastoris over the past fifteen years, including both intracellular and extracellular proteins.
-
(1999)
FEMS Microbiol Rev
-
-
Lin Cereghino, J.1
Cregg, J.M.2
-
13
-
-
0023650956
-
Expression of the LacZ gene from two methanol-regulated promoters in Pichia pastoris
-
Tschopp JF, Brust PF, Cregg JM, Stillman CA, Gingeras TR Expression of the LacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res. 15:1987;3859-3876.
-
(1987)
Nucleic Acids Res
, vol.15
, pp. 3859-3876
-
-
Tschopp, J.F.1
Brust, P.F.2
Cregg, J.M.3
Stillman, C.A.4
Gingeras, T.R.5
-
14
-
-
0031602702
-
Expression of tetanus toxin fragment C
-
D.R. Higgins, & J.M. Cregg. Totowa, NJ: Humana Press
-
Clare J, Sreekrishna K, Romanos M Expression of tetanus toxin fragment C. Higgins DR, Cregg JM Pichia Protocols, vol 103. 1998;193-208 Humana Press, Totowa, NJ.
-
(1998)
Pichia Protocols, Vol 103
, pp. 193-208
-
-
Clare, J.1
Sreekrishna, K.2
Romanos, M.3
-
15
-
-
0031579445
-
Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter
-
Waterham HR, Digan ME, Koutz PJ, Lair SV, Cregg JM Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene. 186:1997;37-44.
-
(1997)
Gene
, vol.186
, pp. 37-44
-
-
Waterham, H.R.1
Digan, M.E.2
Koutz, P.J.3
Lair, S.V.4
Cregg, J.M.5
-
16
-
-
0032541478
-
A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris
-
Shen S, Sulter G, Jeffries TW, Cregg JM A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris. Gene. 216:1998;93-102.
-
(1998)
Gene
, vol.216
, pp. 93-102
-
-
Shen, S.1
Sulter, G.2
Jeffries, T.W.3
Cregg, J.M.4
-
17
-
-
0031604266
-
Secretion of recombinant human insulin-like growth factor I (IGF-1)
-
D.R. Higgins, & J.M. Cregg. Totowa, NJ: Humana Press
-
Brierley RA Secretion of recombinant human insulin-like growth factor I (IGF-1). Higgins DR, Cregg JM Pichia Protocols, vol 103. 1998;149-177 Humana Press, Totowa, NJ.
-
(1998)
Pichia Protocols, Vol 103
, pp. 149-177
-
-
Brierley, R.A.1
-
18
-
-
0030732364
-
Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast Pichia pastoris: Formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase
-
Vuorela A, Myllyharju J, Nissi R, Pihlajaniemi T, Kivirikko KI Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast Pichia pastoris: formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase. EMBO J. 16:1997;6702-6712.
-
(1997)
EMBO J
, vol.16
, pp. 6702-6712
-
-
Vuorela, A.1
Myllyharju, J.2
Nissi, R.3
Pihlajaniemi, T.4
Kivirikko, K.I.5
-
19
-
-
0032946487
-
Positive selection of novel peroxisome biogenesis-defective mutants of the yeast Pichia pastoris
-
Johnson MA, Waterham HR, Ksheminska GP, Fayura LR, Cereghino JL, Stasyk OV, Veenhuis M, Kulachkovsky AR, Sibirny AA, Cregg JM Positive selection of novel peroxisome biogenesis-defective mutants of the yeast Pichia pastoris. Genetics. 151:1999;1379-1391.
-
(1999)
Genetics
, vol.151
, pp. 1379-1391
-
-
Johnson, M.A.1
Waterham, H.R.2
Ksheminska, G.P.3
Fayura, L.R.4
Cereghino, J.L.5
Stasyk, O.V.6
Veenhuis, M.7
Kulachkovsky, A.R.8
Sibirny, A.A.9
Cregg, J.M.10
-
20
-
-
0032526934
-
A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris
-
Sears IB, O'Connor J, Rossanese OW, Glick BS A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris. Yeast. 14:1998;783-790.
-
(1998)
Yeast
, vol.14
, pp. 783-790
-
-
Sears, I.B.1
O'Connor, J.2
Rossanese, O.W.3
Glick, B.S.4
-
21
-
-
0032906221
-
Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis
-
Saliola M, Mazzoni C, Solimando N, Crisa A, Falcone C, Jung G, Fleer R Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis. Appl Environ Microbiol. 65:1999;53-60.
-
(1999)
Appl Environ Microbiol
, vol.65
, pp. 53-60
-
-
Saliola, M.1
Mazzoni, C.2
Solimando, N.3
Crisa, A.4
Falcone, C.5
Jung, G.6
Fleer, R.7
-
22
-
-
0032518664
-
Development of the methylotrophic yeast Pichia methanolica for the expression of the 65 kilodalton isoform of human glutamate decarboxylase
-
Raymond CR, Bukowski T, Holderman SD, Ching AF, Vanaja E, Stamm MR Development of the methylotrophic yeast Pichia methanolica for the expression of the 65 kilodalton isoform of human glutamate decarboxylase. Yeast. 14:1998;11-23.
-
(1998)
Yeast
, vol.14
, pp. 11-23
-
-
Raymond, C.R.1
Bukowski, T.2
Holderman, S.D.3
Ching, A.F.4
Vanaja, E.5
Stamm, M.R.6
-
23
-
-
0031104672
-
Expression level tuning for optimal heterologous protein secretion in Saccharomyces cerevisiae
-
Parekh RN, Wittrup KD Expression level tuning for optimal heterologous protein secretion in Saccharomyces cerevisiae. Biotechnol Prog. 13:1997;117-122.
-
(1997)
Biotechnol Prog
, vol.13
, pp. 117-122
-
-
Parekh, R.N.1
Wittrup, K.D.2
-
24
-
-
0029347117
-
Multicopy overexpresssion of bovine pancreatic trypsin inhibitor saturates protein folding and secretory capacity of Saccharomyces cerevisiae
-
Parekh RN, Forrester K, Wittrup KD Multicopy overexpresssion of bovine pancreatic trypsin inhibitor saturates protein folding and secretory capacity of Saccharomyces cerevisiae. Protein Expr Purif. 6:1995;537-545.
-
(1995)
Protein Expr Purif
, vol.6
, pp. 537-545
-
-
Parekh, R.N.1
Forrester, K.2
Wittrup, K.D.3
-
25
-
-
0030019291
-
An integrating vector for tunable, high copy, stable integration into the dispersed Ty sites of Saccharomyces cerevisiae
-
Parekh RN, Shaw MR, Wittrup KD An integrating vector for tunable, high copy, stable integration into the dispersed Ty sites of Saccharomyces cerevisiae. Biotechnol Prog. 12:1996;16-21.
-
(1996)
Biotechnol Prog
, vol.12
, pp. 16-21
-
-
Parekh, R.N.1
Shaw, M.R.2
Wittrup, K.D.3
-
26
-
-
0029944822
-
Reduction of BiP levels decreases heterologous protein secretion in Saccharomyces cerevisiae
-
Robinson AS, Bockhaus JA, Voegler AC, Wittrup KD Reduction of BiP levels decreases heterologous protein secretion in Saccharomyces cerevisiae. J Biol Chem. 171:1996;10017-10022.
-
(1996)
J Biol Chem
, vol.171
, pp. 10017-10022
-
-
Robinson, A.S.1
Bockhaus, J.A.2
Voegler, A.C.3
Wittrup, K.D.4
-
27
-
-
0031879861
-
Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments
-
The effect of the overexpression of ER residents PDI and BiP are analyzed in terms of their effect on secretion of scFv molecules. Parameters are optimized, including BiP and PDI expression, to give the highest antibody fragment yields.
-
Shusta EV, Raines RT, Pluckthun A, Wittrup KD Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments. Nat Biotechnol. 16:1998;773-777. The effect of the overexpression of ER residents PDI and BiP are analyzed in terms of their effect on secretion of scFv molecules. Parameters are optimized, including BiP and PDI expression, to give the highest antibody fragment yields.
-
(1998)
Nat Biotechnol
, vol.16
, pp. 773-777
-
-
Shusta, E.V.1
Raines, R.T.2
Pluckthun, A.3
Wittrup, K.D.4
-
29
-
-
0030994634
-
Yeast surface display for screening combinatorial polypeptide libraries
-
Boder ET, Wittrup KD Yeast surface display for screening combinatorial polypeptide libraries. Nat Biotechnol. 15:1997;553-558.
-
(1997)
Nat Biotechnol
, vol.15
, pp. 553-558
-
-
Boder, E.T.1
Wittrup, K.D.2
-
30
-
-
85030368782
-
Yeast surface display for directed evolution of protein affinity, stability, and expression
-
in press.
-
Boder ET, Wittrup KD Yeast surface display for directed evolution of protein affinity, stability, and expression. Methods Enzymol. 1999;. in press.
-
(1999)
Methods Enzymol
-
-
Boder, E.T.1
Wittrup, K.D.2
-
31
-
-
0031424522
-
Isolation of anti-T cell receptor scFv mutants by yeast surface display
-
Kieke MC, Cho BK, Boder ET, Kranz DM, Wittrup KD Isolation of anti-T cell receptor scFv mutants by yeast surface display. Protein Eng. 10:1997;1303-1310.
-
(1997)
Protein Eng
, vol.10
, pp. 1303-1310
-
-
Kieke, M.C.1
Cho, B.K.2
Boder, E.T.3
Kranz, D.M.4
Wittrup, K.D.5
-
32
-
-
0031791599
-
A yeast surface display system for the discovery of ligands that trigger cell activation
-
Cho BK, Kieke MC, Boder ET, Wittrup KD, Kranz DM A yeast surface display system for the discovery of ligands that trigger cell activation. J Immunol Methods. 220:1998;179-188.
-
(1998)
J Immunol Methods
, vol.220
, pp. 179-188
-
-
Cho, B.K.1
Kieke, M.C.2
Boder, E.T.3
Wittrup, K.D.4
Kranz, D.M.5
-
33
-
-
0030690331
-
Tag games in yeast: The two-hybrid system and beyond
-
Brachmann RK, Boeke JD Tag games in yeast: the two-hybrid system and beyond. Curr Opin Biotechnol. 8:1997;561-568.
-
(1997)
Curr Opin Biotechnol
, vol.8
, pp. 561-568
-
-
Brachmann, R.K.1
Boeke, J.D.2
-
34
-
-
0032404491
-
The impact of two-hybrid and related methods on biotechnology
-
This review discusses some of the most recent improvements in the basic two-hybrid technique, such as refinements that allow for selection of proteins that select against protein interactions and the use of non-transcription-based selection systems.
-
Colas P, Brent R The impact of two-hybrid and related methods on biotechnology. Trends Biotechnol. 16:1998;355-363. This review discusses some of the most recent improvements in the basic two-hybrid technique, such as refinements that allow for selection of proteins that select against protein interactions and the use of non-transcription-based selection systems.
-
(1998)
Trends Biotechnol
, vol.16
, pp. 355-363
-
-
Colas, P.1
Brent, R.2
-
35
-
-
0033546308
-
A two-hybrid dual bait system to discriminate specificity of protein interactions
-
This paper describes a dual bait version of the two-hybrid system designed to simultaneously identify potential interaction partners and filter out spurious interactions. The ability of this system to eliminate false positives at this early step will save researchers both time and frustration.
-
Serebriiskii I, Khazak V, Golemis EA A two-hybrid dual bait system to discriminate specificity of protein interactions. J Biol Chem. 274:1999;17080-17087. This paper describes a dual bait version of the two-hybrid system designed to simultaneously identify potential interaction partners and filter out spurious interactions. The ability of this system to eliminate false positives at this early step will save researchers both time and frustration.
-
(1999)
J Biol Chem
, vol.274
, pp. 17080-17087
-
-
Serebriiskii, I.1
Khazak, V.2
Golemis, E.A.3
-
36
-
-
0028080090
-
Split ubiquitin as a sensor of protein interactions in vivo
-
Johnsson N, Varshavsky A Split ubiquitin as a sensor of protein interactions in vivo. Proc Natl Acad Sci USA. 91:1994;10340-10344.
-
(1994)
Proc Natl Acad Sci USA
, vol.91
, pp. 10340-10344
-
-
Johnsson, N.1
Varshavsky, A.2
-
37
-
-
0032574840
-
A genetic system based on split-ubiquitin for the analysis of interactions between membrane in vivo
-
The split-ubiquitin system is modified for use in the detection of interactions between membrane proteins. The potential applications and limitations of this system are discussed.
-
Stagljar I, Korostensky C, Johnsson N, teHeesen S A genetic system based on split-ubiquitin for the analysis of interactions between membrane in vivo. Proc Natl Acad Sci USA. 95:1998;5187-5192. The split-ubiquitin system is modified for use in the detection of interactions between membrane proteins. The potential applications and limitations of this system are discussed.
-
(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 5187-5192
-
-
Stagljar, I.1
Korostensky, C.2
Johnsson, N.3
Teheesen, S.4
-
38
-
-
14744295703
-
High-level ATP production by a genetically-engineered Candida yeast
-
Sakai Y, Rogi T, Yonehara T, Kato N, Tani Y High-level ATP production by a genetically-engineered Candida yeast. Bio/Technology. 12:1994;291-293.
-
(1994)
Bio/Technology
, vol.12
, pp. 291-293
-
-
Sakai, Y.1
Rogi, T.2
Yonehara, T.3
Kato, N.4
Tani, Y.5
-
39
-
-
0025096938
-
Kluyveromyces as a host for heterologous gene expression: Expression and secretion of prochymosin
-
Van den Berg JA, Van der Laken KJ, Van Ooyen AJJ, Renniers TC, Rietveld D, Schaap A, Brake AJ, Bishop RJ, Schultz K, Moyer Det al. Kluyveromyces as a host for heterologous gene expression: expression and secretion of prochymosin. Biotechnology. 8:1990;135-139.
-
(1990)
Biotechnology
, vol.8
, pp. 135-139
-
-
Van Den Berg, J.A.1
Van Der Laken, K.J.2
Van Ooyen, A.J.J.3
Renniers, T.C.4
Rietveld, D.5
Schaap, A.6
Brake, A.J.7
Bishop, R.J.8
Schultz, K.9
Moyer, D.10
-
40
-
-
0030071623
-
Expression and secretion of recombinant ovine β-lactoglobulin in Saccharomyces cerevisiae and Kluyveromyces lactis
-
Rocha TL, Paterson G, Crimins K, Boyd A, Sawyer L, Fothergill-Gilmore LA Expression and secretion of recombinant ovine β-lactoglobulin in Saccharomyces cerevisiae and Kluyveromyces lactis. Biochem J. 313:1996;927-932.
-
(1996)
Biochem J
, vol.313
, pp. 927-932
-
-
Rocha, T.L.1
Paterson, G.2
Crimins, K.3
Boyd, A.4
Sawyer, L.5
Fothergill-Gilmore, L.A.6
-
41
-
-
0019958884
-
Alkaline extracellular protease produced by Saccharomyces lipolytica CX161-1B
-
Ogrydziak DM, Scharf SJ Alkaline extracellular protease produced by Saccharomyces lipolytica CX161-1B. J Gen Microbiol. 128:1982;1225-1234.
-
(1982)
J Gen Microbiol
, vol.128
, pp. 1225-1234
-
-
Ogrydziak, D.M.1
Scharf, S.J.2
|