GC-MS analysis of amino acids rapidly provides rich information for isotopomer balancing

Biotechnology Progress

Volumn 16, Issue 4, 2000, Pages 642-649

  Dauner, Michael ^a   Sauer, Uwe ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOLYSIS; ISOTOPOMERS; METABOLIC FLUX ANALYSIS;

BIOCHEMICAL PATHWAY; GAS CHROMATOGRAPHY; GLYCOLYSIS; ISOTOPOMER DISTRIBUTION; MASS SPECTROMETRY; PENTOSE PHOSPHATE; SIMULATION;

BIOMASS; GAS CHROMATOGRAPHY; ISOTOPES; MASS SPECTROMETRY; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

AMINO ACIDS;

AMINO ACIDS; BIOMASS; ESCHERICHIA COLI; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; MODELS, CHEMICAL; SERUM ALBUMIN, BOVINE;

EID: 0034233268     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000058h     Document Type: Article

References (34)

1. Bailey, J. E. Toward a science of metabolic engineering. Science 1991, 252, 1668-1675.
2. 13C NMR, MS and metabolic flux balancing in biotechnology research. Q. Rev. Biophys. 1998, 31, 41-106.
3. 13C-labeling of proteinogenic amino acids. An efficient analytical tool to investigate intermediary metabolism. Eur. J. Biochem. 1995, 232, 433-448.
4. Sauer, U.; Lasko, D. R.; Fiaux, J.; Hochuli, M.; Glaser, R.; Szyperski, T.; Wüthrich, K.; Bailey, J. E. Metabolic flux ratio analysis of genetic and environmental modulations of Escherichia coli central carbon metabolism. J. Bacteriol. 1999, 181, 6679-6688.
5. Zupke, C.; Stephanopoulos, G. Modeling of isotope distributions and intracellular fluxes in metabolic networks using atom mapping matrices. Biotechnol. Prog. 1994, 10, 489-498.
6. Wiechert, W.; de Graaf, A. A. Bidirectional reaction steps in metabolic networks: I. Modeling and simulation of carbon isotope labeling experiments. Biotechnol. Bioeng. 1997, 55, 101-117.
7. Schmidt, K.; Carlsen, M.; Nielsen, J.; Villadsen, J. Modeling isotopomer distributions in biochemical networks using isotopomer mapping matrices. Biotechnol. Bioeng. 1997, 55, 831-840.
8. Marx, A.; de Graaf, A. A.; Wiechert, W.; Eggeling, L.; Sahm, H. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol. Bioeng. 1996, 49, 111-129.
9. 13C coupling constraints on the isotopomer distribution in labeled biomass components. Met. Eng. 1999, 1, 166-179.
10. Christensen, B.; Nielsen, J. Isotopomer analysis using GC - MS. Met. Eng. 1999, 1, 282-290.
11. Poole, C. F.; Sye, W.-F.; Singhawangcha, S.; Hsu, F.; Zlatkis, A.; Arfwidsson, A.; Vessman, J. New electron-capturing pentafluorophenyldialkylchlorosilanes as versatile derivatizing reagents for gas chromatography. J. Chromatogr. 1980, 199, 123-142.
12. Evershed, R. P. Advances in silylation. In Handbook of Derivatives for Chromatography; Blau, K., Halket, J. M. Eds.; John Wiley & Sons: New York, 1993; pp 51-108.
13. Kellner, R.; Meyer, H. E.; Lottspeich, F. Amino acid analysis. In Microcharacterization of Proteins; Kellner, R., Lottspeich, F., Meyer, H. E., Eds.; Wiley-VCH: Weinheim, 1999; pp 119-140.
14. Bentley, R.; Saha, N. C.; Sweeley, C. C. Separation of protium and deuterium forms of carbohydrates by gas chromatography. Anal. Chem. 1965, 37, 1118-1122.
15. Goromaru, T.; Maeda, H. Isotopic fractionation of isopropylantipyrine and its deuterated analogues by capillary gas chromatography. Biol. Pharm. Bull. 1994, 17, 1635-1639.
16. Das Neves, H. J. C.; Vasconcelos, A. M. P. Capillary gas chromatography of amino acids, including asparagine and glutamine: sensitive gas chromatographic-mass spectrometric and selected ion monitoring gas chromatographic-mass spectrometric detection of the N,O(S)-tert.-butyldimethylsilyl derivatives. J. Chromatogr. 1987, 392, 249-258.
17. Hellerstein, M. K.; Neese, R. A. Mass isotopomer distribution analysis at eight years: theoretical, analytic, and experimental considerations. Am. J. Physiol. 1999, 276, E1146-1170.
18. Mook, W. G.; Grootes, P. M. The measuring procedure and corrections for the high-precision mass-spectrometric analysis of isotopic abundance ratios, especially referring to carbon, oxygen and nitrogen. Int. J. Mass Spectrom. Ion Phys. 1973, 12, 273-298.
19. Bergner, E. A.; Lee, W.-N. P. Testing gas chromatographic/ mass spectrometric systems for linearity of response. J. Mass Spectrom. 1995, 30, 778-780.
20. Fagerquist, C. K.; Neese, R. A.; Hellerstein, M. K. Molecular ion fragmentation and its effects on mass isotopomer abundances of fatty acid methyl esters ionized by electron impact. J. Am. Soc. Mass Spectrom. 1999, 10, 430-439.
21. Wiechert, W.; Möllney, M.; Isermann, N.; Wurzel, M.; de Graaf, A. A. Bidirectional reaction steps in metabolic networks: III. Explicit solution and analysis of isotopomer labeling systems. Biotechnol. Bioeng. 1999, 66, 69-85.
22. Wittmann, C.; Heinzle, E. Mass spectrometry for metabolic flux analysis. Biotechnol. Bioeng. 1999, 62, 739-750.
23. Wood, T. The Pentose Phosphate Pathway; Academic Press: Orlando, 1985.
24. Sauer, U.; Hatzimanikatis, V.; Hohmann, H. P.; Manneberg, M.; van Loon, A. P.; Bailey, J. E. Physiology and metabolic fluxes of wild-type and riboflavin-producing Bacillus subtilis. Appl. Environ. Microbiol. 1996, 62, 3687-96.
25. Deines, P. The isotopic composition of reduced organic carbon. In Handbook of Environmental Isotope Geochemistry. The Terrestrial Environment; Fritz, P., Fontes, J. C., Eds.; Elsevier: Amsterdam, 1980; Vol. 1, Chapter 9.
26. Sauer, U.; Hatzimanikatis, V.; Bailey, J. E.; Hochuli, M.; Szyperski, T.; Wüthrich, K. Metabolic fluxes in riboflavin-producing Bacillus subtilis. Nat. Biotechnol. 1997, 15, 448-452.
27. Christensen, B.; Nielsen, J. Metabolic network analysis. A powerful tool in metabolic engineering. Adv. Biochem. Eng. Biotechnol. 2000, 66, 209-31.
28. Sauer, U.; Szyperski, T.; Bailey, J. E. Future trends in complex microbial reaction studies. In NMR in Microbiology: Theory and Applications; Horizon Scientific Press: Wymondham, 2000; pp 483-494.
29. Möllney, M.; Wiechert, W.; Kownatzki, D.; de Graaf, A. A. Bidirectional reaction steps in metabolic networks: IV. Optimal design of isotopomer labeling experiments. Biotechnol. Bioeng. 1999, 66, 86-103.
30. Wiechert, W.; Siefke, C.; de Graaf, A.; Marx, A. Bidirectional reaction steps in metabolic networks: II. Flux estimation and statistical analysis. Biotechnol. Bioeng. 1997, 55, 118-135.
31. 13C]glycerol and mass isotopomer distribution analysis of glucose. Am. J. Physiol. 1995, 269, 516-523.
32. 13C mass isotopomer distributions for natural stable isotope abundance. J. Mass Spectrom. 1996, 31, 255-262.
33. Chinkes, D. L.; Aarsland, A.; Rosenblatt, J.; Wolfe, R. R. Comparison of mass isotopomer dilution methods used to compute VLDL production in vivo. Am. J. Physiol. 1996, 271, E373-383.
34. Husek, P. Amino acid derivatization and analysis in five minutes. FEBS Lett. 1991, 280, 354-356.