Von Hippel-Lindau β-domain-luciferase fusion protein as a bioluminescent hydroxyproline sensor for a hypoxia-inducible factor prolyl hydroxylase assay

Analytical Biochemistry

Volumn 407, Issue 2, 2010, Pages 220-225

  Hong, Sungchae ^a   Yum, Soohwan ^a   Ha, Nam Chul ^a   Jung, Yunjin ^a  

^a Pusan National University   (South Korea)

Author keywords

HIF prolyl hydroxylase; HIF prolyl hydroxylase activity assay; Hypoxia inducible factor; Luciferase; Recombinant VHL luciferase fusion protein; Von Hippel Lindau

Indexed keywords

ENZYME ACTIVITY; ENZYME INHIBITION; ESCHERICHIA COLI; HYDROXYLATION; HYDROXYPROLINE; PEPTIDES;

FUSION PROTEINS; HYPOXIA-INDUCIBLE FACTORS; LUCIFERASE; PROLYL HYDROXYLASE; VON HIPPEL LINDAU;

RECOMBINANT PROTEINS;

2 OXOGLUTARIC ACID; DIMETHYLOXALYLGLYCINE; GLUTATHIONE TRANSFERASE; GLYCINE DERIVATIVE; HYBRID PROTEIN; HYDROXYPROLINE; HYPOXIA INDUCIBLE FACTOR; HYPOXIA INDUCIBLE FACTOR 1ALPHA; HYPOXIA INDUCIBLE FACTOR PROLYL HYDROXYLASE; HYPOXIA INDUCIBLE FACTOR PROLYL HYDROXYLASE INHIBITOR; LUCIFERASE; OXYGENASE INHIBITOR; PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE; RECOMBINANT PROTEIN; RECOMBINANT VON HIPPEL LINDAU PROTEIN BETA LUCIFERASE FUSION PROTEIN; UNCLASSIFIED DRUG; VON HIPPEL LINDAU PROTEIN;

ARTICLE; BINDING AFFINITY; BIOASSAY; BIOLUMINESCENCE; BIOSENSOR; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DRUG BINDING; ENZYME ACTIVITY; ENZYME INHIBITION; ESCHERICHIA COLI; HUMAN; HUMAN CELL; HYDROXYLATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PURIFICATION; WESTERN BLOTTING;

ESCHERICHIA COLI;

EID: 77957236341     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.08.013     Document Type: Article

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