A nonradioactive 96-well plate assay for the detection of hypoxia-inducible factor prolyl hydroxylase activity

Analytical Biochemistry

Volumn 330, Issue 1, 2004, Pages 74-80

  Oehme, Felix ^a   Jonghaus, Willi ^b   Narouz Ott, Laila ^b   Huetter, Joachim ^a   Flamme, Ingo ^a  

^a BAYER PHARMA AG   (Germany)

^b Institute Enabling Technologies   (Germany)

Author keywords

ELISA; HIF; Hypoxia inducible factor; Prolyl hydroxylase; VHL; Von Hippel Lindau protein

Indexed keywords

ESCHERICHIA COLI; MAMMALS; PEPTIDES; TRANSCRIPTION;

ELISA; HYPOXIA-INDUCIBLE FACTORS; HYPOXIA-INDUCIBLE TRANSCRIPTION FACTORS; NANOMOLAR CONCENTRATION; PROLYL HYDROXYLASE; PROTEASOMAL DEGRADATION; TRANSCRIPTIONAL ACTIVATIONS; VON HIPPEL LINDAU;

HYDROXYLATION;

HYPOXIA INDUCIBLE FACTOR PROLINE HYDROXYLASE; HYPOXIA INDUCIBLE FACTOR PROLINE HYDROXYLASE 3; PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE; PROTEIN ANTIBODY; STREPTAVIDIN; THIOREDOXIN; THIOREDOXIN ANTIBODY; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; VON HIPPEL LINDAU PROTEIN; VON HIPPEL LINDAU PROTEIN ELONGIN B ELONGIN C COMPLEX;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BIOTINYLATION; ENZYME ACTIVITY; ESCHERICHIA COLI; HYDROXYLATION; MICROTITER PLATE ASSAY; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN PURIFICATION;

ESCHERICHIA COLI; MAMMALIA;

EID: 2942579553     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.03.066     Document Type: Article

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