Analysis of Microtubules in Budding Yeast

Methods in Cell Biology

Volumn 97, Issue C, 2010, Pages 277-306

  Rauch, Alexander ^a   Nazarova, Elena ^b   Vogel, Jackie ^b  

^a ETH ZURICH   (Switzerland)

^b MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; TUBULIN;

BIOLOGICAL MODEL; BOOK; CHEMISTRY; CYTOLOGY; DIAGNOSIS, MEASUREMENT AND ANALYSIS; METABOLISM; METHODOLOGY; MICROSCOPY; MICROTUBULE; SACCHAROMYCETALES; ULTRASTRUCTURE;

GREEN FLUORESCENT PROTEINS; LABORATORY TECHNIQUES AND PROCEDURES; MICROSCOPY; MICROTUBULES; MODELS, BIOLOGICAL; RECOMBINANT FUSION PROTEINS; SACCHAROMYCETALES; TUBULIN;

EID: 77955644239     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0091-679X(10)97016-7     Document Type: Book

References (189)

1. Adames N.R., Cooper J.A. Microtubule interactions with the cell cortex causing nuclear movements in Saccharomyces cerevisiae. J. Cell Biol. 2000, 149(4):863-874.
2. Akhmanova A., Hoogenraad C.C. Microtubule plus-end-tracking proteins: Mechanisms and functions. Curr. Opin. Cell Biol. 2005, 17:47-54.
3. Akhmanova A., Steinmetz M. Tracking the ends: A dynamic protein network controls the fate of microtubule tips. Nat. Rev. Mol. Cell Biol. 2008, 9:309-322.
4. Al-Bassam J., van Breugel M., Harrison S.C., Hyman A. Stu2p binds tubulin and undergoes an open-to-closed conformational change. J. Cell Biol. 2006, 172:1009-1022.
5. Allingham J.S., Sproul L.R., Rayment I., Gilbert S.P. Vik1 modulates microtubule-Kar3 interactions through a motor domain that lacks an active site. Cell 2007, 128:1161-1172.
6. Amos L., Klug A. Arrangement of subunits in flagellar microtubules. J. Cell Sci. 1974, 14:523-549.
7. Badin-Larcon A.C., Boscheron C., Soleilhac J.M., Piel M., Mann C., Denarier E., Fourest Lieuvin A., Lafanechère L., Bornens M., Job D. Suppression of nuclear oscillations in Saccharomyces cerevisiae expressing Glu tubulin. Proc. Natl. Acad. Sci. U.S.A. 2004, 101(15):5577-5582.
8. Beach D.L., Thibodeaux J., Maddox P., Yeh E., Bloom K. The role of the proteins Kar9 and Myo2 in orienting the mitotic spindle of budding yeast. Curr. Biol. 2000, 10:1497-1506.
9. Berlin V., Styles C.A., Fink G.R. BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin. J. Cell Biol. 1990, 111:2573-2586.
10. Bicek A.D., Tüzel E., Kroll D.M., Odde D.J. Analysis of microtubule curvature. Methods Cell Biol. 2007, 83:237-268.
11. Bieling P., Laan L., Schek H., Munteanu E.L., Sandblad L., Dogterom M., Brunner D., Surrey T. Reconstitution of a microtubule plus-end tracking system in vitro. Nature 2007, 450:1100-1105.
12. Bienz M. Spindles cotton on to junctions, APC and EB1. Nat. Cell Biol. 2001, 3E.
13. Brachmann C.B., Davies A., Cost G.J., Caputo E., Li J., Hieter P., Boeke J.D. Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 1998, 14:115-132.
14. Brangwynne C.P., Koenderink G.H., Barry E., Dogic Z., MacKintosh F.C., Weitz D.A. Bending dynamics of fluctuating biopolymers probed by automated high-resolution filament tracking. Biophys. J. 2007, 93(1):346-359.
15. Busch K.E., Brunner D. The microtubule plus end-tracking proteins mal3p and tip1p cooperate for cell-end targeting of interphase microtubules. Curr. Biol. 2004, 14:548-559.
16. Busch K.E., Hayles J., Nurse P., Brunner D. Tea2p kinesin is involved in spatial microtubule organization by transporting tip1p on microtubules. Dev. Cell 2004, 6:831-843.
17. Cardinale J., Rauch A., Barral Y., Szekely G., Sbalzarini I. Bayesian image analysis with on-line confidence estimates and its application to microtubule tracking. Biomedical Imaging: From Nano to Macro, 2009 ISBI '09 IEEE International Symposium on 2009, 1091-1094.
18. Carminati J.L., Stearns T. Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex. J. Cell Biol. 1997, 138(3):629-641.
19. Carvalho P., Gupta M.L., Hoyt M.A., Pellman D. Cell cycle control of kinesin-mediated transport of bik1 (CLIP-170) regulates microtubule stability and dynein activation. Dev. Cell 2004, 6:815-829.
20. Carvalho P., Tirnauer J.S., Pellman D. Surfing on microtubule ends. Trends Cell Biol. 2003, 13(5):229-237.
21. Caudron F., Andrieux A., Job D., Boscheron C. A new role for kinesin-directed transport of bik1p (CLIP-170) in Saccharomyces cerevisiae. J. Cell. Sci. 2008, 121:1506-1513.
22. Chen X.P., Yin H., Huffaker T.C. The yeast spindle pole body component spc72p interacts with stu2p and is required for proper microtubule assembly. J. Cell Biol. 1998, 141:1169-1179.
23. Combs C.A. Fluorescence microscopy: A concise guide to current imaging methods. Curr. Protoc. Neurosci. 2010, Chapter 2, 2.1.1-2.1.14.
24. Costanzo M., Baryshnikova A., Bellay J., Kim Y., Spear E.D., Sevier C.S., Ding H., Koh J.L., Toufighi K., Mostafavi S., et al. The genetic landscape of a cell. Science 2010, 327:425-431.
25. Cuschieri L., Miller R., Vogel J. Gamma-tubulin is required for proper recruitment and assembly of kar9-bim1 complexes in budding yeast. Mol. Biol. Cell 2006, 17:4420-4434.
26. Danuser G. Super-resolution microscopy using normal flow decoding and geometric constraints. J. Microsc. 2001, 204:136-149.
27. Danuser G., Tran P.T., Salmon E.D. Tracking differential interference contrast diffraction line images with nanometre sensitivity. J. Microsc. 2000, 198(Pt 1):34-53.
28. De Wulf P., McAinsh A.D., Sorger P.K. Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev. 2003, 17:2902-2921.
29. DeZwaan T.M., Ellingson E., Pellman D., Roof D.M. Kinesin-related KIP3 of Saccharomyces cerevisiae is required for a distinct step in nuclear migration. J. Cell Biol. 1997, 138:1023-1040.
30. Dorn J.F., Danuser G., Yang G. Computational processing and analysis of dynamic fluorescence image data. Methods Cell Biol. 2008, 85:497-538.
31. Dorn J.F., Jaqaman K., Rines D.R., Jelson G.S., Sorger P.K., Danuser G. Yeast kinetochore microtubule dynamics analyzed by high-resolution three-dimensional microscopy. Biophys. J. 2005, 89:2835-2854.
32. Endow S.A., Kang S.J., Satterwhite L.L., Rose M.D., Skeen V.P., Salmon E.D. Yeast kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBO J. 1994, 13:2708-2713.
33. Eshel D., Urrestarazu L.A., Vissers S., Jauniaux J.C., van Vliet-Reedijk J.C., Planta R.J., Gibbons I.R. Cytoplasmic dynein is required for normal nuclear segregation in yeast. Proc. Natl. Acad. Sci. U.S.A. 1993, 90:11172-11176.
34. Fridman V., Gerson-Gurwitz A., Movshovich N., Kupiec M., Gheber L. Midzone organization restricts interpolar microtubule plus-end dynamics during spindle elongation. EMBO Rep. 2009, 10:387-393.
35. Gao Y., Kilfoil M.L. Accurate detection and complete tracking of large populations of features in three dimensions. Opt. Express 2009, 17:4685-4704.
36. Gard D.L., Becker B.E., Josh Romney S. MAPping the eukaryotic tree of life: Structure, function, and evolution of the MAP215/dis1 family of microtubule-associated proteins. Int. Rev. Cytol. 2004, 239:179-272.
37. Gardner M.K., Bouck D.C., Paliulis L.V., Meehl J.B., O'Toole E.T., Haase J., Soubry A., Joglekar A.P., Winey M., Salmon E.D., et al. Chromosome congression by kinesin-5 motor-mediated disassembly of longer kinetochore microtubules. Cell 2008, 135:894-906.
38. Gardner M.K., Haase J., Mythreye K., Molk J.N., Anderson M., Joglekar A.P., O'Toole E.T., Winey M., Salmon E.D., Odde D.J., et al. The microtubule-based motor kar3 and plus end-binding protein bim1 provide structural support for the anaphase spindle. J. Cell Biol. 2008, 180:91-100.
39. Gardner M.K., Hunt A.J., Goodson H.V., Odde D.J. Microtubule assembly dynamics: New insights at the nanoscale. Curr. Opin. Cell Biol. 2008, 20:64-70.
40. Gardner M.K., Odde D.J. Stochastic simulation and graphic visualization of mitotic processes. Methods 2010, 51(2):251-256.
41. Gardner M.K., Odde D.J., Bloom K. Hypothesis testing via integrated computer modeling and digital fluorescence microscopy. Methods 2007, 41:232-237.
42. Gardner M.K., Pearson C.G., Sprague B.L., Zarzar T.R., Bloom K., Salmon E.D., Odde D.J. Tension-dependent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast. Mol. Biol. Cell 2005, 16:3764-3775.
43. Geissler S., Pereira G., Spang A., Knop M., Soues S., Kilmartin J., Schiebel E. The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment. EMBO J. 1996, 15:3899-3911.
44. Gigant B., Curmi P.A., Martin-Barbey C., Charbaut E., Lachkar S., Lebeau L., Siavoshian S., Sobel A., Knossow M. The 4A X-ray structure of a tubulin: Stathmin-like domain complex. Cell 2000, 102:809-816.
45. Gräf R., Rietdorf J., Zimmermann T. Live cell spinning disk microscopy. Adv. Biochem. Eng. Biotechnol. 2005, 95:57-75.
46. Grava S., Schaerer F., Faty M., Philippsen P., Barral Y. Asymmetric recruitment of dynein to spindle poles and microtubules promotes proper spindle orientation in yeast. Dev. Cell 2006, 10:425-439.
47. Gregoretti I.V., Margolin G., Alber M.S., Goodson H.V. Insights into cytoskeletal behavior from computational modeling of dynamic microtubules in a cell-like environment. J. Cell Sci. 2006, 119(Pt 22):4781-4788.
48. Gupta M.L., Carvalho P., Roof D.M., Pellman D. Plus end-specific depolymerase activity of kip3, a kinesin-8 protein, explains its role in positioning the yeast mitotic spindle. Nat. Cell Biol. 2006, 8:913-923.
49. Gustafsson M.G. Extended resolution fluorescence microscopy. Curr. Opin. Struct. Biol. 1999, 9:627-634.
50. Hadjidemetriou S., Toomre D., Duncan J.S. Segmentation and 3D reconstruction of microtubules in total internal reflection fluorescence microscopy (TIRFM). Med. Image Comput. Comput. Assist. Interv. 2005, 8(Pt 1):761-769.
51. Hadjidemetriou S., Toomre D., Duncan J. Motion tracking of the outer tips of microtubules. Med. Image Anal. 2008, 12(6):689-702.
52. Heemskerk J.W.T., Westra A.H., Linotte P.M., Ligtvoet K.M., Zbijewski W., Beekman F.J. Front-illuminated versus back-illuminated photon-counting CCD-based gamma camera: Important consequences for spatial resolution and energy resolution. Phys. Med. Biol. 2007, 52(8).
53. Heintzmann R., Ficz G. Breaking the resolution limit in light microscopy. Methods Cell Biol. 2007, 81:561-580.
54. Hell S.W. Toward fluorescence nanoscopy. Nat. Biotechnol. 2003, 21:1347-1355.
55. Hinsch J. Mating cameras to microscopes. Methods Cell Biol. 2007, 81:55-61.
56. Howard J., Hyman A.A. Dynamics and mechanics of the microtubule plus end. Nature 2003, 422:753-758.
57. Howard J., Hyman A.A. Microtubule polymerases and depolymerases. Curr. Opin. Cell Biol. 2007, 19:31-35.
58. Howard J., Hyman A.A. Growth, fluctuation and switching at microtubule plus ends. Nat. Rev. Mol. Cell Biol. 2009, 10:569-574.
59. Huh W.K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Global analysis of protein localization in budding yeast. Nature 2003, 425:686-691.
60. Huisman S.M., Bales O.A., Bertrand M., Smeets M.F., Reed S.I., Segal M. Differential contribution of Bud6p and Kar9p to microtubule capture and spindle orientation in S. cerevisiae. J. Cell Biol. 2004, 167:231-244.
61. Huisman S.M., Segal M. Cortical capture of microtubules and spindle polarity in budding yeast-where's the catch?. J. Cell Sci. 2005, 118:463-471.
62. 6 Temporal and spatio-temporal sampling problems. J. ICRU 2006, 6:65-76.
63. Janson M.E., Loughlin R., Loïodice I., Fu C., Brunner D., Nédélec F.J., Tran P.T. Crosslinkers and motors organize dynamic microtubules to form stable bipolar arrays in fission yeast. Cell 2007, 128(2):357-368.
64. Jaqaman K., Danuser G. Linking data to models: Data regression. Nat. Rev. Mol. Cell Biol. 2006, 7:813-819.
65. Jaqaman K., Dorn J.F., Jelson G.S., Tytell J.D., Sorger P.K., Danuser G. Comparative autoregressive moving average analysis of kinetochore microtubule dynamics in yeast. Biophys. J. 2006, 91(6):2312-2325.
66. Jaqaman K., Dorn J.F., Marco E., Sorger P.K., Danuser G. Phenotypic clustering of yeast mutants based on kinetochore microtubule dynamics. Bioinformatics 2007, 23:1666-1673.
67. Jaqaman K., Loerke D., Mettlen M., Kuwata H., Grinstein S., Schmid S.L., Danuser G. Robust single-particle tracking in live-cell time-lapse sequences. Nat. Methods 2008, 5:695-702.
68. Jiang M., Ji Q., McEwen B. Enhancement of microtubules in EM tomography. Biomedical Imaging: Nano to Macro, 2004 IEEE International Symposium on 2004, 1122:1123-1126.
69. Jiang M., Ji Q., McEwen B. Automated extraction of microtubules and their plus-ends. Application of Computer Vision, 2005 WACV/MOTIONS '05 Volume 1 Seventh IEEE Workshops on 2005, 336-341.
70. Karsenti E., Nédélec F., Surrey T. Modelling microtubule patterns. Nat. Cell Biol. 2006, 8(11):1204-1211.
71. Katz W., Weinstein B., Solomon F. Regulation of tubulin levels and microtubule assembly in Saccharomyces cerevisiae: Consequences of altered tubulin gene copy number. Mol. Cell. Biol. 1990, 10:5286-5294.
72. Kerssemakers J., Ionov L., Queitsch U., Luna S., Hess H., Diez S. 3D nanometer tracking of motile microtubules on reflective surfaces. Small 2009, 5(15):1732-1737.
73. Kerssemakers J.W., Munteanu E.L., Laan L., Noetzel T.L., Janson M.E., Dogterom M. Assembly dynamics of microtubules at molecular resolution. Nature 2006, 442:709-712.
74. Khmelinskii A., Lawrence C., Roostalu J., Schiebel E. Cdc14-regulated midzone assembly controls anaphase B. J. Cell Biol. 2007, 177(6):981-993.
75. Kilmartin J.V., Adams A.E. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces. J. Cell Biol. 1984, 98(3):922-933.
76. Knaus M., Cameroni E., Pedruzzi I., Tatchell K., De Virgilio C., Peter M. The Bud14p-Glc7p complex functions as a cortical regulator of dynein in budding yeast. EMBO J. 2005, 24:3000-3011.
77. Knop M., Pereira G., Geissler S., Grein K., Schiebel E. The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication. EMBO J. 1997, 16:1550-1564.
78. Knop M., Pereira G., Schiebel E. Microtubule organization by the budding yeast spindle pole body. Biol. Cell. 1999, 91:291-304.
79. Korinek W.S., Copeland M.J., Chaudhuri A., Chant J. Molecular linkage underlying microtubule orientation toward cortical sites in yeast. Science 2000, 287(5461):2257-2259.
80. Kosco K.A., Pearson C.G., Maddox P.S., Wang P.J., Adams I.R., Salmon E.D., Bloom K., Huffaker T.C. Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast. Mol. Biol. Cell 2001, 12:2870-2880.
81. Kurihara L.J., Beh C.T., Latterich M., Schekman R., Rose M.D. Nuclear congression and membrane fusion: Two distinct events in the yeast karyogamy pathway. J. Cell Biol. 1994, 126:911-923.
82. Kusch J., Meyer A., Snyder M.P., Barral Y. Microtubule capture by the cleavage apparatus is required for proper spindle positioning in yeast. Genes Dev 2002, 16:1627-1639.
83. Lansbergen G., Akhmanova A. Microtubule plus end: A hub of cellular activities. Traffic 2006, 7:499-507.
84. Lee W. The role of the lissencephaly protein Pac1 during nuclear migration in budding yeast. J. Cell Biol. 2003, 160:355-364.
85. Lee W.L., Kaiser M.A., Cooper J. The offloading model for dynein function: Differential function of motor subunits. J. Cell Biol. 2005, 168:201-207.
86. Lee L., Tirnauer J.S., Li J., Schuyler S.C., Liu J.Y. Positioning of the mitotic spindle by a cortical-microtubule capture mechanism. Science 2000, 287(5461):2260-2262.
87. Leisner C., Kammerer D., Denoth A., Britschi M., Barral Y., Liakopoulos D. Regulation of mitotic spindle asymmetry by SUMO and the spindle-assembly checkpoint in yeast. Curr. Biol. 2008, 18:1249-1255.
88. Li J., Lee W.L., Cooper J. NudEL targets dynein to microtubule ends through LIS1. Nat. Cell Biol. 2005, 7:686-690.
89. Li Y.Y., Yeh E., Hays T., Bloom K. Disruption of mitotic spindle orientation in a yeast dynein mutant. Proc. Natl. Acad. Sci. U.S.A. 1993, 90:10096-10100.
90. Liakopoulos D., Kusch J., Grava S., Vogel J., Barral Y. Asymmetric loading of Kar9 onto spindle poles and microtubules ensures proper spindle alignment. Cell 2003, 112:561-574.
91. Lichtenstein N., Geiger B., Kam Z. Quantitative analysis of cytoskeletal organization by digital fluorescent microscopy. Cytometry A 2003, 54(1):8-18.
92. Lichtman J.W., Conchello J.-A. Fluorescence microscopy. Nat. Methods 2005, 2(12):910-919.
93. Lin H., de Carvalho P., Kho D., Tai C.Y., Pierre P., Fink G.R., Pellman D. Polyploids require Bik1 for kinetochore-microtubule attachment. J. Cell Biol. 2001, 155:1173-1184.
94. Lowe J., Li H., Downing K.H., Nogales E. Refined structure of alpha beta-tubulin at 3.5A resolution. J. Mol. Biol. 2001, 313:1045-1057.
95. Ma L., McQueen J., Cuschieri L., Vogel J., Measday V. Spc24 and Stu2 promote spindle integrity when DNA replication is stalled. Mol. Biol. Cell 2007, 18:2805-2816.
96. Maddox P.S., Bloom K.S., Salmon E.D. The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae. Nat. Cell Biol. 2000, 2:36-41.
97. Maddox P.S., Stemple J.K., Satterwhite L., Salmon E.D., Bloom K. The minus end-directed motor Kar3 is required for coupling dynamic microtubule plus ends to the cortical shmoo tip in budding yeast. Curr. Biol. 2003, 13:1423-1428.
98. Maekawa H., Schiebel E. Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex. Genes Dev. 2004, 18:1709-1724.
99. Maekawa H., Usui T., Knop M., Schiebel E. Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions. EMBO J. 2003, 22:438-449.
100. Manning B.D., Barrett J.G., Wallace J.A., Granok H., Snyder M. Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p. J. Cell Biol. 1999, 144:1219-1233.
101. Marcus A.I. Visualization of spindle behavior using confocal microscopy. Methods Mol. Med. 2007, 137:125-137.
102. Marschall L.G., Jeng R.L., Mulholland J., Stearns T. Analysis of Tub4p, a yeast gamma-tubulin-like protein: Implications for microtubule-organizing center function. J. Cell Biol. 1996, 134:443-454.
103. Meluh P.B., Rose M.D. KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell 1990, 60:1029-1041.
104. Mendoza M., Norden C., Durrer K., Rauter H., Uhlmann F., Barral Y. A mechanism for chromosome segregation sensing by the NoCut checkpoint. Nat. Cell Biol 2009, 11(4):477-483.
105. Mennella V., Rogers G.C., Rogers S.L., Buster D.W., Vale R.D., Sharp D.J. Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase. Nat. Cell Biol. 2005, 7:235-245.
106. Middleton K., Carbon J. KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore. Proc. Natl. Acad. Sci. U.S.A. 1994, 91:7212-7216.
107. Miller R.K., Cheng S.C., Rose M.D. Bim1p/Yeb1p mediates the Kar9p-dependent cortical attachment of cytoplasmic microtubules. Mol. Biol. Cell 2000, 11:2949-2959.
108. Miller R.K., D'Silva S., Moore J., Goodson H.V. The CLIP-170 orthologue Bik1p and positioning the mitotic spindle in yeast. Curr. Top. Dev. Biol. 2006, 76:49-87.
109. Miller R.K., Heller K.K., Frisen L., Wallack D.L., Loayza D., Gammie A.E., Rose M.D. The kinesin-related proteins, Kip2p and Kip3p, function differently in nuclear migration in yeast. Mol. Biol. Cell 1998, 9:2051-2068.
110. Miller R., Matheos D., Rose M.D. The Cortical Localization of the Microtubule Orientation Protein, Kar9p, Is Dependent upon Actin and proteins required for polarization. J. Cell Biol. 1999, 144(5):963-975.
111. Miller R., Rose M.D. Kar9p Is a Novel Cortical Protein Required for Cytoplasmic Microtubule Orientation in Yeast. J. Cell Biol. 1998, 140(2):377-390.
112. Molk J.N., Bloom K. Microtubule dynamics in the budding yeast mating pathway. J. Cell. Sci. 2006, 119:3485-3490.
113. Molk J.N., Salmon E.D., Bloom K. Nuclear congression is driven by cytoplasmic microtubule plus end interactions in S. cerevisiae. J. Cell Biol. 2006, 172:27-39.
114. Moomaw B. Camera technologies for low light imaging: Overview and relative advantages. Methods Cell Biol. 2007, 81:251-283.
115. Moore J.K., D'Silva S., Miller R.K. The CLIP-170 homologue Bik1p promotes the phosphorylation and asymmetric localization of Kar9p. Mol. Biol. Cell 2006, 17:178-191.
116. Moore J.K., Miller R.K. The cyclin-dependent kinase Cdc28p regulates multiple aspects of Kar9p function in yeast. Mol. Biol. Cell 2007, 18:1187-1202.
117. Moore J.K., Stuchell-Brereton M.D., Cooper J.A. Function of dynein in budding yeast: Mitotic spindle positioning in a polarized cell. Cell Motil. Cytoskeleton 2009, 66:546-555.
118. Morrison E.E. Action and interactions at microtubule ends. Cell Mol. Life Sci. 2007, 64:307-317.
119. Nédélec F. Computer simulations reveal motor properties generating stable antiparallel microtubule interactions. J. Cell Biol. 2002, 158(6):1005-1015.
120. Neff N.F., Thomas J.H., Grisafi P., Botstein D. Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo. Cell 1983, 33:211-219.
121. Nogales E. Structural insight into microtubule function. Annu. Rev. Biophys. Biomol. Struct. 2001, 30:397-420.
122. Odde D.J., Buettner H.M. Time series characterization of simulated microtubule dynamics in the nerve growth cone. Ann. Biomed. Eng. 1995, 23:268-286.
123. Odde D.J., Buettner H.M., Cassimeris L. Spectral analysis of microtubule assembly dynamics. AIChE J. 1996, 42:1434-1442.
124. Ohkura H., Garcia M.A., Toda T. Dis1/TOG universal microtubule adaptors-one MAP for all?. J. Cell. Sci. 2001, 114(Pt21):3805-3812.
125. Page B.D., Satterwhite L.L., Rose M.D., Snyder M. Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. J. Cell Biol. 1994, 124:507-519.
126. Pearson C.G., Gardner M.K., Paliulis L.V., Salmon E.D., Odde D.J., Bloom K. Measuring nanometer scale gradients in spindle microtubule dynamics using model convolution microscopy. Mol. Biol. Cell 2006, 17(9):4069-4079.
127. Pinot M., Chesnel F., Kubiak J., Arnal I., Nedelec F., Gueroui Z. Effects of confinement on the self-organization of microtubules and motors. Curr. Biol. 2009, 19(11):954-960.
128. Rasnik I., French T., Jacobson K., Berland K. Electronic cameras for low-light microscopy. Methods Cell Biol. 2007, 81:219-249.
129. Ravelli R.B., Gigant B., Curmi P.A., Jourdain I., Lachkar S., Sobel A., Knossow M. Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain. Nature 2004, 428:198-202.
130. Rout M.P., Kilmartin J.V. Components of the yeast spindle and spindle pole body. J. Cell Biol. 1990, 111:1913-1927.
131. Sage D., Neumann F., Hediger F., Gasser S., Unser M. Automatic tracking of individual fluorescence particles: Application to the study of chromosome dynamics. IEEE Trans. Image Process 2005, 14(9):1372-1383.
132. Sagot I., Klee S.K., Pellman D. Yeast formins regulate cell polarity by controlling the assembly of actin cables. Nat. Cell Biol. 2002, 4:42-50.
133. Salmon E.D., von Lackum K., Canman J.C. Proper alignment and adjustment of the light microscope. Curr. Protoc. Microbiol. Chapter 2, Unit 2, A 1. 2005.
134. Saunders W., Hornack D., Lengyel V., Deng C. The Saccharomyces cerevisiae kinesin-related motor Kar3p acts at preanaphase spindle poles to limit the number and length of cytoplasmic microtubules. J. Cell Biol. 1997, 137:417-431.
135. Sbalzarini I.F., Koumoutsakos P. Feature point tracking and trajectory analysis for video imaging in cell biology. J. Struct. Biol. 2005, 151(2):182-195.
136. Schatz P.J., Pillus L., Grisafi P., Solomon F., Botstein D. Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins. Mol. Cell. Biol. 1986, 6:3711-3721.
137. Schatz P.J., Solomon F., Botstein D. Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins. Mol. Cell. Biol. 1986, 6:3722-3733.
138. Schek H.T., Gardner M.K., Cheng J., Odde D.J., Hunt A.J. Microtubule assembly dynamics at the nanoscale. Curr. Biol. 2007, 17:1445-1455.
139. Schulz R.B., Semmler W. Fundamentals of optical imaging. Handb. Exp. Pharmacol. 2008, 185(Pt 1):3-22.
140. Schuyler S.C., Pellman D. Microtubule "plus-end-tracking proteins": The end is just the beginning. Cell 2001, 105:421-424.
141. Schwartz K., Richards K., Botstein D. BIM1 encodes a microtubule-binding protein in yeast. Mol. Biol. Cell 1997, 8:2677-2691.
142. Segal M., Bloom K. Control of spindle polarity and orientation in Saccharomyces cerevisiae. Trends Cell Biol. 2001, 11:160-166.
143. Segal M., Bloom K., Reed S.I. Bud6 directs sequential microtubule interactions with the bud tip and bud neck during spindle morphogenesis in Saccharomyces cerevisiae. Mol. Biol. Cell 2000, 11:3689-3702.
144. Shariff A., Murphy R.F., Rohde G.K. A generative model of microtubule distributions, and indirect estimation of its parameters from fluorescence microscopy images. Cytometry A 2010, 77:457-466.
145. Shaw S.L., Maddox P., Skibbens R.V., Yeh E., Salmon E.D., Bloom K. Nuclear and spindle dynamics in budding yeast. Mol. Biol. Cell 1998, 9(7):1627-1631.
146. Shaw S.L., Yeh E., Maddox P., Salmon E.D., Bloom K. Astral microtubule dynamics in yeast: A microtubule-based searching mechanism for spindle orientation and nuclear migration into the bud. J. Cell Biol. 1997, 139(4):985-994.
147. Sheeman B., Carvalho P., Sagot I., Geiser J., Kho D., Hoyt M.A., Pellman D. Determinants of S. cerevisiae dynein localization and activation: Implications for the mechanism of spindle positioning. Curr. Biol. 2003, 13:364-372.
148. Shelden E., Knecht D.A. Reconstruction and display of curvilinear objects from optical section data using 3-D curve fitting algorithms. J. Microsc. 1998, 191(Pt 1):97-107.
149. Smal I., Draegestein K., Galjart N., Niessen W., Meijering E. Rao-Blackwellized marginal particle filtering for multiple object tracking in molecular bioimaging. Inf. Process Med. Imaging 2007, 20:110-121.
150. Smal I., Draegestein K., Galjart N., Niessen W., Meijering E. Particle filtering for multiple object tracking in dynamic fluorescence microscopy images: Application to microtubule growth analysis. IEEE Trans. Med. Imaging 2008, 27(6):789-804.
151. Smal I., Meijering E., Draegestein K., Galjart N., Grigoriev I., Akhmanova A., van Royen M.E., Houtsmuller A.B., Niessen W. Multiple object tracking in molecular bioimaging by Rao-Blackwellized marginal particle filtering. Med. Image Anal. 2008, 12(6):764-777.
152. Sobel S.G., Snyder M. A highly divergent gamma-tubulin gene is essential for cell growth and proper microtubule organization in Saccharomyces cerevisiae. J. Cell Biol. 1995, 131:1775-1788.
153. Soues S., Adams I.R. SPC72: A spindle pole component required for spindle orientation in the yeast Saccharomyces cerevisiae. J. Cell. Sci. 1998, 111(Pt 18):2809-2818.
154. Spang A., Geissler S., Grein K., Schiebel E. gamma-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation. J. Cell Biol. 1996, 134:429-441.
155. Sprague B.L., Pearson C.G., Maddox P.S., Bloom K.S., Salmon E.D., Odde D.J. Mechanisms of microtubule-based kinetochore positioning in the yeast metaphase spindle. Biophys. J. 2003, 84(6):3529-3546.
156. Spring K.R. Cameras for digital microscopy. Methods Cell Biol. 2007, 81:171-186.
157. Sproul L.R., Anderson D.J., Mackey A.T., Saunders W.S., Gilbert S.P. Cik1 targets the minus-end kinesin depolymerase kar3 to microtubule plus ends. Curr. Biol. 2005, 15:1420-1427.
158. Stelzer E.H.K. Practical Limits to Resolution in Fluorescence Light Microscopy 1999, Cold Spring Harbor Laboratory Press, New York.
159. Straight A.F., Marshall W.F., Sedat J.W., Murray A.W. Mitosis in living budding yeast: Anaphase A but no metaphase plate. Science 1997, 277:574-578.
160. Straight A.F., Sedat J.W., Murray A.W. Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast. J. Cell Biol. 1998, 143:687-694.
161. Tarassov K., Messier V., Landry C.R., Radinovic S., Serna Molina M.M., Shames I., Malitskaya Y., Vogel J., Bussey H., Michnick S.W. An in vivo map of the yeast protein interactome. Science 2008, 320:1465-1470.
162. Taylor D.L., Salmon E.D. Basic fluorescence microscopy. Methods Cell Biol. 1989, 29:207-237.
163. Thomann D., Dorn J., Sorger P.K., Danuser G. Automatic fluorescent tag localization II: Improvement in super-resolution by relative tracking. J. Microsc. 2003, 211:230-248.
164. Thomann D., Rines D.R., Sorger P.K., Danuser G. Automatic fluorescent tag detection in 3D with super-resolution: application to the analysis of chromosome movement. J. Microsc. 2002, 208:49-64.
165. Tirnauer J.S., Bierer B.E. EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability. J. Cell Biol. 2000, 149:761-766.
166. Tirnauer J.S., Grego S., Salmon E.D., Mitchison T.J. EB1-microtubule interactions in Xenopus egg extracts: Role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules. Mol. Biol. Cell 2002, 13:3614-3626.
167. Tirnauer J.S., O'Toole E., Berrueta L., Bierer B.E., Pellman D. Yeast Bim1p promotes the G1-specific dynamics of microtubules. J. Cell Biol. 1999, 145:993-1007.
168. Tischer C., Brunner D., Dogterom M. Chapter 20: Automated spatial mapping of microtubule catastrophe rates in fission yeast. Methods Cell Biol. 2008, 89:521-538.
169. Trueheart J., Boeke J.D., Fink G.R. Two genes required for cell fusion during yeast conjugation: Evidence for a pheromone-induced surface protein. Mol. Cell. Biol. 1987, 7:2316-2328.
170. Tytell J.D., Sorger P.K. Analysis of kinesin motor function at budding yeast kinetochores. J. Cell Biol. 2006, 172:861-874.
171. Usui T., Maekawa H., Pereira G., Schiebel E. The XMAP215 homologue Stu2 at yeast spindle pole bodies regulates microtubule dynamics and anchorage. EMBO J. 2003, 22:4779-4793.
172. Van Breugel M., Drechsel D., Hyman A. Stu2p, the budding yeast member of the conserved Dis1/XMAP215 family of microtubule-associated proteins is a plus end-binding microtubule destabilizer. J. Cell Biol. 2003, 161(2):359-369.
173. Varga V., Helenius J., Tanaka K., Hyman A., Tanaka T.U., Howard J. Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nat. Cell Biol. 2006, 8:957-962.
174. Varga V., Leduc C., Bormuth V., Diez S., Howard J. Kinesin-8 motors act cooperatively to mediate length-dependent microtubule depolymerization. Cell 2009, 138:1174-1183.
175. Vogel J., Drapkin B., Oomen J., Beach D., Bloom K., Snyder M. Phosphorylation of gamma-tubulin regulates microtubule organization in budding yeast. Dev. Cell 2001, 1(5):621-631.
176. Vogel J., Snyder M. gamma-Tubulin of budding yeast. Curr. Top. Dev. Biol. 2000, 49:75-104.
177. Wang P.J., Huffaker T.C. Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body. J. Cell Biol. 1997, 139:1271-1280.
178. Waters J.C. Live-cell fluorescence imaging. Methods Cell Biol. 2007, 81:115-140.
179. Wigge P.A., Jensen O.N., Holmes S., Soues S., Mann M., Kilmartin J.V. Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J. Cell Biol. 1998, 141:967-977.
180. Winzeler E.A., Shoemaker D.D., Astromoff A., Liang H., Anderson K., Andre B., Bangham R., Benito R., Boeke J.D., Bussey H., et al. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 1999, 285(5429):901-906.
181. Wolf D.E. Fundamentals of fluorescence and fluorescence microscopy. Methods Cell Biol. 2007, 81:63-91.
182. Wolyniak M.J., Blake-Hodek K., Kosco K., Hwang E., You L., Huffaker T.C. The regulation of microtubule dynamics in Saccharomyces cerevisiae by three interacting plus-end tracking proteins. Mol. Biol. Cell 2006, 17:2789-2798.
183. Woodruff J.B., Drubin D.G., Barnes G. Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment. Mol. Biol. Cell 2009, 20:3003-3011.
184. Wu X., Xiang X., Hammer J.A. Motor proteins at the microtubule plus-end. Trends Cell Biol. 2006, 16(3):135-143.
185. Yamamoto A., Hiraoka Y. Cytoplasmic dynein in fungi: Insights from nuclear migration. J. Cell Sci. 2003, 116:4501-4512.
186. Yeh E., Skibbens R.V., Cheng J.W., Salmon E.D., Bloom K. Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae. J. Cell Biol. 1995, 130:687-700.
187. Yeh E., Yang C., Chin E., Maddox P., Salmon E.D., Lew D.J., Bloom K. Dynamic positioning of mitotic spindles in yeast: Role of microtubule motors and cortical determinants. Mol. Biol. Cell 2000, 11:3949-3961.
188. Yin H., Pruyne D., Huffaker T.C., Bretscher A. Myosin V orientates the mitotic spindle in yeast. Nature 2000, 406:1013-1015.
189. Zaichick S.V., Metodiev M.V., Nelson S.A., Durbrovskyi O., Draper E., Cooper J.R., Stone D.E. The Mating-specific G{alpha} Interacts with a Kinesin-14 and Regulates Pheromone-induced Nuclear Migration in Budding Yeast. Mol. Biol. Cell 2009, 20(12):2820-2830.