The Saccharomyces cerevisiae kinesin-related motor Kar3p acts at preanaphase spindle poles to limit the number and length of cytoplasmic microtubules

Journal of Cell Biology

Volumn 137, Issue 2, 1997, Pages 417-431

  Saunders, William ^a   Hornack, David ^a   Lengyel, Valerie ^a   Deng, Changchun ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA TUBULIN; BENOMYL; FUNGAL PROTEIN; KINESIN; NOCODAZOLE;

ARTICLE; CELL CYCLE; CYTOPLASM; FUNGAL GENETICS; FUNGUS GROWTH; MICROTUBULE; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; ANAPHASE; BASE SEQUENCE; BENOMYL; FUNGAL PROTEINS; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MITOTIC SPINDLE APPARATUS; MOLECULAR SEQUENCE DATA; MUTATION; NOCODAZOLE; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0030933383     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.137.2.417     Document Type: Article

References (38)

1. Belmont, L.D., A.A. Hyman, K.E. Sawin, and T.J. Mitchison. 1990. Real-time visualization of cell cycle dependent changes in microtubule dynamics in cytoplasmic extracts. Cell. 62:579-589.
2. Buendia, B., G. Draetta, and E. Karsenti. 1992. Regulation of the microtubule nucleating activity of centrosomes in Xenopus egg extracts: role of cyclin A-associated protein kinase. J. Cell Biol. 116:1431-1442.
3. Davidse, L.C. 1986. Benzimadazole fungicides: mechanism of action and biological impact. Annu. Rev. Phytopathol. 24:43-65.
4. De Brabander, M. 1986. Microtubule dynamics during the cell cycle: the effects of taxol and nocodazole on the microtubule system of PtK2 cells at different stages of the mitotic cycle. Int. Rev. Cytol. 101:215-274.
5. Desai, A., and T. Mitchison. 1995. A new role for motor proteins as couplers to depolymerizing microtubules. J. Cell Biol. 128: 1-4.
6. Endow, S., S. Kang, L. Satterwhite, M. Rose, V. Skeen, and E. Salmon. 1994. Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBO (Eur. Mol. Biol. Organ.) J. 13:2708-2713.
7. Goh, P., and J.V. Kilmartin. 1993. NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae. J. Cell Biol. 121:503-512.
8. Gotoh, Y., E. Nishida, and S. Matsuda. 1991. In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase. Nature (Lond.). 349:251-254.
9. Harlow, E., and D. Lane. 1988. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
10. Hoyt, M.A., L. Totis, and B.T. Roberts. 1991. S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell. 66:507-517.
11. Hoyt, M.A., L. He, K.K. Loo, and W.S. Saunders. 1992. Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly. J. Cell Biol. 118:109-120.
12. Inoue, S. 1981. Cell division and the mitotic spindle. J. Cell Biol. 91:131s-147s.
13. Li, R., and A.W. Murray. 1991. Feedback control of mitosis in budding yeast. Cell. 66:519-531.
14. Lombillo, V.A., C. Nislow, T.J. Yen, V.I. Gelfand, and J.R. McIntosh. 1995a. Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro. J. Cell Biol. 128:107-115.
15. Lombillo, V.A., R.J. Stewart, and J.R. McIntosh. 1995b. Minus-end-directed motion of kinesin-coated microspheres driven by microtubule depolymerization. Nature (Lond.). 373:161-165.
16. Machin, N.A., J.M. Lee, and G. Barnes. 1995. Microtubule stability in budding yeast: characterization and dosage suppression of a benomyl-dependent tubulin mutant. Mol. Biol. Cell. 6:1241-1259.
17. Margolis, R.L., and L. Wilson. 1978. Opposite end assembly and disassembly of microtubules at steady state in vitro. Cell. 13:1-8.
18. Masuda, H., K. McDonald, and W.Z. Cande. 1988. The mechanism of anaphase spindle elongation: uncoupling of tubulin incorporation and microtubule sliding during in vitro spindle elongation. J. Cell Biol. 107:623-633.
19. Meluh, P.B., and M.D. Rose. 1990. KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell. 60:1029-1041.
20. Middleton, K., and J. Carbon. 1994. Kar3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore. Proc. Natl. Acad. Sci. USA. 91:7212-7216.
21. Mitchison, T.J. 1989. Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence. J. Cell Biol. 109:637-652.
22. Neff, N.F., J.H. Thomas, P. Grisafi, and D. Bolstein. 1983. Isolation of the β-tubulin gene from yeast and demonstration of its essential function in vivo. Cell. 33: 211-219.
23. Page, B.D., L.L. Satterwhite, M.D. Rose, and M. Snyder. 1994. Localization of the Kar3 kinesin heavy chain-like protein requires the CIK1 interacting protein. J. Cell Biol. 124:507-519.
24. Peterson, J.B., and H. Ris. 1976. Electron-microscopic study of the spindle and chromosome movement in the yeast Saccharomyces cerevisiae. J. Cell Sci. 22: 219-242.
25. Pringle, J.R., and L.H. Hartwell. 1981. The Saccharomyces cerevisiae Cell Cycle. In The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance. J.N. Strathern, E.W. Jones, and J.R. Broach, editors. Cold Spring Harbor Laboratory, pp.97-142.
26. Pringle, J.R., A.E.M. Adams, D.G. Drubin, and B.K. Haarer. 1991. Immunofluorescence methods for yeast. Methods Enzymol. 194:565-602.
27. Roof, D.M., P.E. Meluh, and M.D. Rose. 1991. Multiple kinesin-related proteins in yeast mitosis. Cold Spring Harbor Symp. Quant. Biol. 56:693-703.
28. Rout, M.P., and J.V. Kilmartin. 1990. Components of the yeast spindle and spindle pole body. J. Cell Biol. 111:1913-1927.
29. Salmon, E.D., M. McKeel, and T. Hays. 1984. Rapid rate of tubulin dissociation from microtubules in the mitotic spindle in vivo measured by blocking polymerization with colchicine. J. Cell Biol. 99:1066-1075.
30. Saunders, W.S., and M.A. Hoyt. 1992. Kinesin-related proteins required for structural integrity of the mitotic spindle. Cell. 70:451-458.
31. Saunders, W.S., and M.A. Hoyt. 1997. Mitotic spindle function in Saccharomyces cerevisiae requires a balance between different types of kinesin-related motors. Mol. Cell. Biol. In press.
32. Sawin, K.E., and T.J. Mitchison. 1991. Poleward microtubule flux in mitotic spindles assembled in vitro. J. Cell Biol. 112:941-954.
33. Schatz, P.J., F. Solomon, and D. Botstein. 1986. Genetically essential and non-essential α-tubulin genes specify functionally interchangeable proteins. Mol. Cell. Biol. 6:3722-3733.
34. Sherman, F., G.R. Fink, and J.B. Hicks. 1983. Methods in Yeast Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
35. Vale, R.D., and L.S.B. Goldstein. 1990. One motor, many tails: an expanding repertoire of force-generating enzymes. Cell. 60:883-885.
36. Walczak, C., T. Mitchison, and A. Desai. 1996. XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. Cell. 84:37-47.
37. Wells, W.A, and A.W. Murray. 1996. Aberrantly segregating centromeres activate the spindle assembly checkpoint in budding yeast. J. Cell Biol. 133:75-84.
38. Zhang, D., and R.B. Nicklas. 1996. "Anaphase" and cytokinesis in the absence of chromosomes. Nature (Lond.). 382:466-468.