-
11
-
-
0032560079
-
-
S. Busson, D. Dujardin, A. Moreau, J. Dompierre, J. R. DeMay, Curr. Biol. 8, 541 (1998).
-
(1998)
Curr. Biol.
, vol.8
, pp. 541
-
-
Busson, S.1
Dujardin, D.2
Moreau, A.3
Dompierre, J.4
DeMay, J.R.5
-
13
-
-
0342265157
-
-
P. Gonczy, S. Pihchler, M. Kirkham, A. A. Hyman, J. Cell Biol. 147, 135 (1999).
-
(1999)
J. Cell Biol.
, vol.147
, pp. 135
-
-
Gonczy, P.1
Pihchler, S.2
Kirkham, M.3
Hyman, A.A.4
-
14
-
-
0030928162
-
-
T. DeZwaan, E. Ellingson, D. Pellman, D. M. Roof, J. Cell Biol. 138, 1023 (1997).
-
(1997)
J. Cell Biol.
, vol.138
, pp. 1023
-
-
DeZwaan, T.1
Ellingson, E.2
Pellman, D.3
Roof, D.M.4
-
17
-
-
0033535351
-
-
L. Lee, S. K. Klee, M. Evangelista, C. Boone, D. Pellman, J. Cell Biol. 144, 947 (1999).
-
(1999)
J. Cell Biol.
, vol.144
, pp. 947
-
-
Lee, L.1
Klee, S.K.2
Evangelista, M.3
Boone, C.4
Pellman, D.5
-
21
-
-
0033620650
-
-
J. S. Tirnauer, E. O'Toole, L. Berrueta, B. E. Bierer, D. Pellman, J. Cell Biol. 145, 993 (1999).
-
(1999)
J. Cell Biol.
, vol.145
, pp. 993
-
-
Tirnauer, J.S.1
O'Toole, E.2
Berrueta, L.3
Bierer, B.E.4
Pellman, D.5
-
23
-
-
0343687478
-
-
Supplemental material is available at www. sciencemag.org/feature/data/1047337.shl
-
-
-
-
26
-
-
0030927327
-
-
H. Imamura et al., EMBO J. 16, 2745 (1997).
-
(1997)
EMBO J.
, vol.16
, pp. 2745
-
-
Imamura, H.1
-
27
-
-
0032482399
-
-
L. Muhua, N. R. Adames, M. D. Murphy, C. R. Shields, J. A. Cooper, Nature 393, 487 (1998).
-
(1998)
Nature
, vol.393
, pp. 487
-
-
Muhua, L.1
Adames, N.R.2
Murphy, M.D.3
Shields, C.R.4
Cooper, J.A.5
-
28
-
-
0343251808
-
-
note
-
The delivery of Bim1p to cortical Kar9p binding sites could signal the successful completion of spindle positioning and promote cytokinesis on schedule. However, we found that Kar9p was not required for the cytokinesis checkpoint (L. Lee and D. Pellman, unpublished data).
-
-
-
-
29
-
-
0032585647
-
-
E. E. Morrison, B. N. Wardleworth, J. M. Askham, A. F. Markham, D. M. Meredith, Oncogene 17, 3471 (1998).
-
(1998)
Oncogene
, vol.17
, pp. 3471
-
-
Morrison, E.E.1
Wardleworth, B.N.2
Askham, J.M.3
Markham, A.F.4
Meredith, D.M.5
-
30
-
-
0029066006
-
-
L.-K. Su et al., Cancer Res. 55, 2972 (1995).
-
(1995)
Cancer Res.
, vol.55
, pp. 2972
-
-
Su, L.-K.1
-
33
-
-
0343687475
-
-
note
-
An astral microtubule was considered correctly oriented if it extended into the bud or mating projection. Analysis was limited to microtubules of length equal to or greater than the shortest distance from the centrosome to the GFP-Kar9p signal. The percentage of preanaphase cells that met the above criteria was 89% in the BIM1 strain (n = 100) and 8% in the bim1Δ strain (n = 228). In α factor-arrested cells, the percentage was 89% in the BIM1 strain (n = 141) and 27% in the bim1Δ strain (n = 321). It is likely that misoriented microtubules in bim1Δ cells are misoriented because they fail to interact with Kar9p. If this is the case, then Bim1p is required for microtubule ends to interact with Kar9p in most cells.
-
-
-
-
34
-
-
0342817243
-
-
note
-
Cell extracts for Western analysis and immunoprecipitations were prepared by glass bead lysis in a buffer containing 150 mM NaCl, 50 mM Tris (pH 8.0), 1% Nonidet P-40, and protease inhibitors. Antibodies for immunoblotting: HA-Bim1p was detected with 12CA5, GFP-Kar9p was detected with a polydonal antibody to GFP, and tubulin was detected with a monoclonal antibody to α tubulin.
-
-
-
-
35
-
-
0342817242
-
-
note
-
Sucrose gradients (5 to 20%) were made in 50 mM Tris (pH 8.0) and 150 mM NaCl. We loaded 400 μl of low-speed supernatant on top of each 12.5-ml gradient and spun it overnight in a Beckman SW40 Ti at 218,000g. The linearity of the gradients was confirmed by refractometry, and Svedberg values were calculated on the basis of globular standards. Gel filtration was performed using a Bio-Rad SE-1000 analytical Gel Filtration column.
-
-
-
-
38
-
-
0034708459
-
-
W. S. Korinek, M. J. Copeland, A. Chaudhuri, J. Chant, Science 287, 2257 (2000).
-
(2000)
Science
, vol.287
, pp. 2257
-
-
Korinek, W.S.1
Copeland, M.J.2
Chaudhuri, A.3
Chant, J.4
-
40
-
-
0343687474
-
-
note
-
We thank J. DeCaprio, E. Elion, J. Kahana, R. Miller, M. Rose, and P. Silver for reagents, E. Elion, R. Li, T. Look, and M. McLaughlin for comments on the manuscript, J. Chant and M. Rose for communicating results before publication, and B. Bierer and members of the Pellman laboratory for discussions. Supported by grants from NIH [GM55772 to D.P. and KO8 DK02578 to J.S.T.] and a Kimmel Scholar Award to D.P.
-
-
-
|