Fluorescence microscopy

Nature Methods

Volumn 2, Issue 12, 2005, Pages 910-919

  Lichtman, Jeff W ^a   Conchello, José Angel ^b,c  

^a HARVARD UNIVERSITY   (United States)

^b OKLAHOMA MEDICAL RESEARCH FOUNDATION   (United States)

^c UNIVERSITY OF OKLAHOMA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCEIN ISOTHIOCYANATE; GREEN FLUORESCENT PROTEIN; YELLOW FLUORESCENT PROTEIN;

ANALYTICAL ERROR; BLEACHING; CELL MEMBRANE PERMEABILITY; ENERGY TRANSFER; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; ILLUMINATION; IMAGE ANALYSIS; IMAGE QUALITY; MOLECULAR BIOLOGY; PHOTOCHEMISTRY; PRIORITY JOURNAL; REVIEW;

ANIMALS; FLUORESCENT DYES; HUMANS; MICROSCOPY, FLUORESCENCE;

EID: 30944468609     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth817     Document Type: Review

References (35)

1. Tsien, R.Y. The green fluorescent protein. Annu. Rev. Biochem. 67, 509-544 (1998).
2. Valeur, B. Molecular Fluorescence: Principles and Applications. (Wiley-VCH, Weinheim 2002).
3. Lakowicz, J.R., ed. Principles of Fluorescence Spectroscopy. 2nd ed. (Plenum Press, New York, 1999).
4. Turro N.J. Modern Molecular Photochemistry. (University Science Books, Sausalito, California, 1991).
5. Irvine, D.J., Purbhoo, M.A., Krogsgaard, M. & Davis, M.M. Direct observation of ligand recognition by T cells. Nature 419, 845-849 (2002).
6. Wang, L., Jackson, W.C., Steinbach, P.A. & Tsien, R.Y. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Natl. Acad. Sci. USA 101, 16745-16749 (2004).
7. Deerinck, T.J. et al. Fluorescence photooxidation with eosin: A method for high resolution immunolocalization and in situ hybridization detection for light and electron microscopy. J. Cell Biol. 126, 901-910 (1994).
8. Suhling, K., French, P.M. & Phillips, D. Time-resolved fluorescence microscopy. Photochem. Photobiol. Sci. 4, 13-22 (2005).
9. Elson, D. et al. Time-domain fluorescence lifetime imaging applied to biological tissue. Photochem. Photobiol. Sci. 3, 795-801 (2004).
10. Jares-Erijman, E.A. & Jovin, T.M. FRET imaging. Nat. Biotechnol. 21, 1387-1395 (2003).
11. Mizuno, H. et al. Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein. Mol. Cell 12, 1051-1058 (2003).
12. Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H. & Miyawaki, A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. USA 99, 12651-12656 (2002).
13. Mason, W.T., ed. Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide for Quantitative Real-Time Analysis. (Academic Press Harcourt Brace and Co., Boston, 1993).
14. Periasamy, A., ed. Methods in Cellular Imaging (Oxford University Press, Oxford, 2001).
15. Rudolf, R., Mongillo, M., Rizzuto, R. & Pozzan, T. Looking forward to seeing calcium. Nat. Rev. Mol. Cell Biol. 4, 579-586 (2003).
16. Tsien, R.Y. Fluorescent indicators of ion concentrations. Methods Cell Biol. 30, 127-156 (1989).
17. Bassnett, S., Reinisch, L. & Beebe, D.C. Intracellular pH measurement using single excitation-dual emission fluorescence ratios. Am. J. Physiol. 258, 171-178 (1990).
18. Yuste, R. & Konnerth, A., eds. Imaging in Neuroscience and Development: A Laboratory Manual. (Cold Spring Harbor Laboratory Press, New York, 2005).
19. Zhang, J., Campbell, R.E., Ting, A.Y. & Tsien, R.Y. Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3, 906-918 (2002).
20. Griesbeck, O. Fluorescent proteins as sensors for cellular functions. Curr. Opin. Neurobiol. 14, 636-641 (2004).
21. Ohmichi, T. et al. DNA-based biosensor for monitoring pH in vitro and in living cells. Biochemistry 44, 7125-7130 (2005).
22. Grinvald, A. & Hildesheim, R. VSDI: A new era in functional imaging of cortical dynamics. Nat. Rev. Neurosci. 5, 874-885 (2004).
23. Kuhn, B., Fromherz, P. & Denk, W. High sensitivity of Stark-shift voltage-sensing dyes by one- or two-photon excitation near the real spectral edge. Biophys. J. 87, 631-639 (2004).
24. Reiff, D.F. et al. In vivo performance of genetically encoded indicators of neural activity in flies. J. Neurosci. 25, 4766-4778 (2005).
25. Goldman, R.D. & Spector, D.L, eds. Live Cell Imaging: A Laboratory Manual. (Cold Spring Harbor Laboratory Press, New York. 2004).
26. Inoué, S. & Spring, K.R. Video Microscopy. 2nd edn. (Plenum Publishing, New York, 1997).
27. Abramowitz, M., Spring, K.R., Keller, H.E. & Davidson, M.W. Basic principles of microscope objectives. Biotechniques 33, 772-781 (2002).
28. Panchuk-Voloshina, N. et al. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright photostable conjugates. J. Histochem. Cytochem. 47, 1179-1188 (1999).
29. Ono, M. et al. Quantitative comparison of anti-fading mounting media for confocal laser scanning microscopy. J. Histochem. Cytochem. 49, 305-331 (2001).
30. Johnson, G.D. & Nogueira Araujo, G.M. A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods 43, 349-350 (1981).
31. Johnson, G.D. et al. Fading of immunofluorescence during microscopy: A study of the phenomenon and its remdedy. J. Immunol. Methods 55, 231-242 (1982).
32. Giloh, H. & Sedat, J.W. Fluorescence microscopy: Reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate. Science 217, 1252-1255 (1982).
33. Alivisatos, A.P., Gu, W. & Larabell, C. Quantum dots as cellular probes. Annu. Rev. Biomed. Eng 7, 55-76 (2005).
34. van Gijlswijk, R.P. et al. Fluorochrome-labeled tyramides: Use in immunocytochemistry and fluorescence in situ hybridization. J. Histochem. Cytochem. 45, 375-382 (1997).
35. Cullander, C. Imaging in the far-red with electronic light microscopy: requirements and limitations. J. Microsc. 176, 281-286 (1994).