Fluorescence microscopy: A concise guide to current imaging methods

Current Protocols in Neuroscience

Volumn , Issue SUPPL.50, 2010, Pages

  Combs, Christian A ^a  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

Confocal; STED; Structured light; Two photon

Indexed keywords

BLEACHING; CONFOCAL LASER MICROSCOPY; DIFFRACTION; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; IMAGE PROCESSING; IMAGE QUALITY; INTERMETHOD COMPARISON; MOLECULAR BIOLOGY; NEUROBIOLOGY; PHOTOTOXICITY; PRIORITY JOURNAL; REVIEW;

ANIMALS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MICROSCOPY, FLUORESCENCE, MULTIPHOTON;

EID: 75949113907     PISSN: 19348584     EISSN: 19348576     Source Type: Journal    
DOI: 10.1002/0471142301.ns0201s50     Document Type: Review

References (23)

1. Boccacci, P., and, Bertero, M., 2002. Image-restoration methods: Basics and algorithms. In Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances (, A. Diaspro, ed.) pp. 253 - 269. Wiley-Liss, New York.
2. Diaspro, A., Bianchini, P., Vicidomini, G., Faretta, M., Ramoino, P., and, Usai, C., 2006. Multi-photon excitation microscopy. Biomed. Eng. Online 5: 36.
3. Eisenstein, M., 2006. Helping cells to tell a colorful tale. Nat. Methods 3: 647 - 655.
4. Gustafsson, M.G., 2000. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J. Microsc. 198: 82 - 87.
5. Gustafsson, M.G., 2005. Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution. Proc. Natl. Acad. Sci. U.S.A. 102: 13081 - 13086.
6. Hell, S.W., 2007. Far-field optical nanoscopy. Science 316: 1153 - 1158.
7. Helmchen, F., and, Denk, W., 2005. Deep tissue two-photon microscopy. Nat. Methods 2: 932 - 940.
8. Hibbs, A.R., 2004. Confocal Microscopy for Biologists. Springer, New York.
9. Inoue, S., and, Spring, K.R., 1997. Video Microscopy: The Fundamentals. Springer, New York.
10. Kellner, R.R., Baier, C.J., Willig, K.I., Hell, S.W., and, Barrantes, F.J., 2007. Nanoscale organization of nicotinic acetylcholine receptors revealed by stimulated emission depletion microscopy. Neuroscience 144: 135 - 43.
11. Lichtman, J.W., and, Conchello, J.A., 2005. Fluorescence microscopy. Nat. Methods 2: 910 - 919.
12. Livet, J., Weissman, T.A., Kang, H., Draft, R.W., Lu, J., Bennis, R.A., Sanes, J.R., and, Lichtman, J.W., 2007. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450: 56 - 62.
13. Pawley, J.B., (Ed.) 2006. Handbook of Biological Confocal Microscopy. Springer, New York.
14. Pearson, H., 2007. The good, the bad and the ugly. Nature 447: 138 - 140.
15. Schermelleh, L., Carlton, P.M., Haase, S., Shao, L., Winoto, L., Kner, P., Burke, B., Cardoso, M.C., Agard, D.A., Gustafsson, M.G., Leonhardt, H., and, Sedat, J.W., 2008. Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Science 320: 1332 - 1336.
16. Shaner, N.C., Steinbach, P.A., and, Tsien, R.Y., 2005. A guide to choosing fluorescent proteins. Nat. Methods 2: 905 - 909.
17. Suzuki, T., Matsuzaki, T., Hagiwara, H., Aoki, T., and, Takata, K., 2007. Recent advances in fluorescent labeling techniques for fluorescence microscopy. Acta Histochem. Cytochem. 40: 131 - 137.
18. Svoboda, K., and, Yasuda, R., 2006. Principles of two-photon excitation microscopy and its applications to neuroscience. Neuron 50: 823 - 839.
19. Toomre, D., and, Manstein, D.J., 2001. Lighting up the cell surface with evanescent wave microscopy. Trends Cell Biol. 11: 298 - 303.
20. Wallace, W., Schaefer, L.H., and, Swedlow, J.R., 2001. A workingpersons guide to deconvolution in light microscopy. Biotechniques 31: 1076 - 1078, 1080, 1082 passim.
21. Willig, K.I., Kellner, R.R., Medda, R., Hein, B., Jakobs, S., and, Hell, S.W., 2006a. Nanoscale resolution in GFP-based microscopy. Nat. Methods 3: 721 - 723.
22. Willig, K.I., Rizzoli, S.O., Westphal, V., Jahn, R., and, Hell, S.W., 2006b. STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis. Nature 440: 935 - 939.
23. Willig, K.I., Harke, B., Medda, R., and, Hell, S.W., 2007. STED microscopy with continuous wave beams. Nat. Methods 4: 915 - 918.