The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly

Journal of Cell Biology

Volumn 141, Issue 5, 1998, Pages 1169-1179

  Chen, Xiaoyue Peter ^a,b   Yin, Hongwei ^a   Huffaker, Tim C ^a  

^a Department of Obstetrics Gynecology   (United States)

^b Columbia University ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; PACLITAXEL;

ARTICLE; CELL MUTANT; CONTROLLED STUDY; CYTOKINESIS; MICROTUBULE ASSEMBLY; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; YEAST CELL;

CELL NUCLEUS; CENTROSOME; FUNGAL PROTEINS; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 0032100813     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.141.5.1169     Document Type: Article

References (42)

1. Ault, J.G., and C.L. Rieder. 1994. Centrosome and kinetochore movement during mitosis. Curr. Opin. Cell Biol. 6:41-49.
2. Baum, P., C. Furlong, and B. Byers. 1986. Yeast gene required for spindle pole body duplication: homology of its product with calcium-binding proteins. Proc. Natl. Acad. Sci. USA. 83:5512-5516.
3. Berlin, V., C. Styles, and G. Fink. 1990. BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin. J. Cell Biol. 111:2573-2586.
4. Boeke, J., F. LaCroute, and G. Fink. 1984. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluorourotic acid resistance. Mol. Gen. Genet. 197:345-346.
5. Bullitt, E., M.P. Rout, J.V. Kilmartin, and C.W. Akey. 1997. The yeast spindle pole body is assembled around a central crystal of Spc42p. Cell. 89:1077-1086.
6. Byers, B., and L. Goetsch. 1975. Behavior of spindles and spindle plaques in the cell cycle and conjugation of saccharomyces cerevisiae. J. Bacteriol. 124:511-523.
7. Byers, B., and L. Goetsch. 1991. Preparation of yeast cells for thin-section electron microscopy. Methods Enzymol. 194:602-607.
8. Christianson, T.W., R.S. Sikorski, M. Dante, J.H. Shero, and P. Hieter. 1992. Multifunctional yeast high-copy-number shuttle vectors. Gene. 110:119-122.
9. Donaldson, A.D., and J.V. Kilmartin. 1996. Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPB) with an essential function during SPB duplication. J. Cell Biol. 132:887-901.
10. Durfee, T., K. Becherer, P.L. Chen, S.H. Yeh, Y. Yang, A.E. Kilburn, W.H. Lee, and S.J. Elledge. 1993. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7:555-569.
11. Geiser, J.R., H.A. Sundberg, B.H. Chang, E.G.D. Muller, and T.N. Davis. 1993. The essential mitotic target of calmodulin is the 110-kilodalton component of the spindle pole body in Saccharomyces cerevisiae. MoIl Cell. Biol. 13: 7913-7924.
12. Geissler, S., G. Pereira, A. Spang, M. Knop, S. Soues, J. Kilmartin, and E. Schiebet. 1996. The spindle pole body component Spc98p interacts with the γ-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment. EMBO (Eur. Mol. Biol. Organ.) J. 15:3899-3911.
13. Heim, R., A.B. Cubitt, and R.Y. Tsien. 1995. Improved green fluorescence. Nature. 373:663-664.
14. Hoyt, M.A., L. He, K.K. Loo, and W.S. Saunders. 1992. Two Saccharomyces cerevisiae kinesin-related gene-products required for mitotic spindle assembly. J. Cell Biol. 118:109-120.
15. Hutter, K.-J., and H.E. Eipel. 1979. Microbial determinations by flow cytometry. J. Gen. Microbiol. 113:369-375.
16. Jensen, O.N., P.A. Wigge, S. Holmes, S. Soues, M. Mann, and J.V. Kilmartin. 1998. Analysis of the Saccharomyces spindle pole by MALDI mass spectrometry. J. Cell Biol. In press.
17. Kilmartin, J.V., S.L. Dyos, D. Kershaw, and J.T. Finch. 1993. A spacer protein in the Saccharomyces cerevisiae spindle whose transcript is cell cycle regulated. J. Cell Biol. 123:1175-1184.
18. Knop, M., G. Pereira, S. Geissler, K. Grein, and E. Schiebel. 1997. The spindle pole body component Spc97p interacts with the γ-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication. EMBO (Eur. Mol. Biol. Organ.) J. 16:1550-1564.
19. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature. 227:680-685.
20. Liu, H., J. Krizek, and A. Bretscher. 1992. Construction of a GAL1-regulated yeast cDNA expression library and its application to the identification of genes whose overexpression causes lethality in yeast. Genetics. 132:665-673.
21. Lupas, A., M. VanDyke, and J. Stock. 1991. Predicting coiled coils from protein sequences. Science. 252:1162-1164.
22. Marschall, L.G., R.L. Jeng, J. Mulholland, and T. Stearns. 1996. Analysis of Tub4p, a yeast γ-tubulin-like protein: implications for microtubule-organizmg center function. J. Cell Biol. 134:443-454.
23. Mitchison, T., and M. Kirschner. 1984. Dynamic instability of microtubule growth. Nature. 312:237-242.
24. Muhlrad, D., R. Hunter, and R. Parker. 1992. A rapid method for localized mutagenesis of yeast genes. Yeast. 8:79-82.
25. Pasqualone, D., and T.C. Huffaker. 1994. STU1, a suppressor of a β-tubulin mutation, encodes a novel and essential component of the yeast mitotic spindle. J. Cell Biol. 127:1973-1984.
26. Pellman, D., M. Bagget, Y.H. Tu, and G.R. Fink. 1995. Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae. J. Cell Biology. 130:1373-1385.
27. Rose, M., and G. Fink. 1987. KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast. Cell. 48:1047-1060.
28. Schatz, P., L. Pillus, P. Grisafi, F. Solomon, and D. Botstein. 1986. Two functional α-tubulin genes of the yeast Saccharomyuces cerevisiae encode divergent proteins. Mol. Cell. Biol. 6:3711-3721.
29. Schwartz, K., K. Richards, and D. Botstein. 1997. BIM1 encodes a microtubule-binding protein in yeast. Mol. Biol. Cell. 8:2677-2691.
30. Sherman, F. 1991. Getting started with yeast. Methods Enzymol. 194:3-21.
31. Sikorski, R., and J. Boeke. 1991. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant yeast. Methods Enzymol. 194:302-318.
32. Sikorski, R.S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:19-27.
33. Sobel, S.G., and M. Snyder. 1995. A highly divergent γ-tubulin gene is essential for cell growth and proper microtubule organization in Saccharomyces cerevisiae. J. Cell Biol. 131:1775-1788.
34. Sorger, P.K., F.F. Severin, and A.A. Hyman. 1994. Factors required for the binding of reassembled yeast kinetochores to microtubules in vitro. J. Cell Biol. 127:995-1008.
35. Spang, A., I. Courtney, U. Fackler, M. Matzner, and E. Schiebel. 1993. The calcium-binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half-bridge of the spindle pole body. J. Cell Biol. 123:405-416.
36. Spang, A., I. Courtney, K. Grein, M. Matzner, and E. Schiebel. 1995. The Cdc31p-binding protein Kar1p is a component of the half-bridge of the yeast spindle pole body. J. Cell Biol. 128:863-877.
37. Spang, A., S. Geissler, K. Grein, and E. Schiebel. 1996. γ-tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation. J. Cell Biol. 134:429-441.
38. Sullivan, D.S., and T.C. Huffaker. 1992. Astral microtubules are not required for anaphase B in Saccharomyces cerevisiae. J. Cell Biol. 119:379-388.
39. Vallen, E.A., T.Y. Scherson, T. Roberts, K. van Zee, and M.D. Rose. 1992. Asymmetric mitotic segregation of the yeast spindle pole body. Cell. 69:505-515.
40. Vernos, I., and E. Karsemi. 1996. Motors involved in spindle assembly and chromosome segregation. Curr. Opin. Cell Biol. 8:4-9.
41. Wang, P.J., and T.C. Huffaker. 1997. Stu2p: a microtubule-binding protein that is an essential component of the yeast spindle pole body. J. Cell Biol. 139: 1271-1280.
42. Weinstein, B., and F. Solomon. 1990. Phenotypic consequences of tubulin over-production In Saccharomyces cerevisiae: differences between alpha-tubulin and beta-tubulin. Mol. Cell. Biol. 10:5295-5304.