Use of multi-staining flow cytometry to characterise the physiological state of Escherichia coli W3110 in high cell density fed-batch cultures

Biotechnology and Bioengineering

Volumn 63, Issue 6, 1999, Pages 705-711

  Hewitt, Christopher J ^a   Nebe Von Caron, Gerhard ^b   Nienow, Alvin W ^a   McFarlane, Caroline M ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b UNILEVER RESEARCH   (United Kingdom)

Author keywords

Escherichia coli; Fed batch; Flow cytometry; High cell density; Membrane integrity; Membrane potential; Viability

Indexed keywords

BACTERIA; BIOLOGICAL MEMBRANES; BIOSYNTHESIS; CYTOLOGY; ESCHERICHIA COLI; FERMENTATION; GLUCOSE; GROWTH KINETICS; MASS SPECTROMETRY; PHYSIOLOGY;

HIGH CELL DENSITY FED BATCH CULTURES; MEMBRANE INTEGRITY; MEMBRANE POTENTIAL; MULTI STAINING FLOE CYTOMETRY;

BATCH CELL CULTURE;

GLUCOSE;

ARTICLE; BACTERIUM CULTURE; CELL DEATH; CELL DENSITY; CELL VIABILITY; ESCHERICHIA COLI; FERMENTATION; FLOW CYTOMETRY; MASS SPECTROMETRY; MEMBRANE POTENTIAL; NONHUMAN;

BIOLOGICAL MARKERS; BIOTECHNOLOGY; CELL MEMBRANE PERMEABILITY; ESCHERICHIA COLI; ETHIDIUM; FERMENTATION; FLOW CYTOMETRY; FLUORESCENT DYES; PROPIDIUM; REPRODUCIBILITY OF RESULTS; THIOBARBITURATES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0033587372     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990620)63:6<705::AID-BIT8>3.0.CO;2-M     Document Type: Article

References (26)

1. Ackermann JU, Müller S, Lösche A, Bley T, Babel, W. 1995. Methylobacterium rhodesianum cells tend to double the DNA content under growth limitations and accumulate PHB. J Biotechnol 39:9-20.
2. Al-Rubeai M, Singh RP, Goldman MH, Emery AN. 1995. Death mechanisms of animal cells in conditions of intensive agitation. Biotech Bioeng 45:463-472.
3. Al-Rubeai M, Emery AN, editors. 1996. Flow cytometry applications in cell culture. New York: Marcel Dekker Inc. 331 p.
4. Andersson LC. 1996. Escherichia coli high cell density fed batch cultures: Kinetic and physiological aspects. PhD thesis. Stockholm, Sweden. Royal Institute of Technology.
5. Andersson LC, Strandberg L, Enfors S. 1996. Cell segregation and lysis have profound effects on growth of Escherichia coli in high cell density fed batch cultures. Biotechnol Prog 12:190-195.
6. Andersson LC, Strandberg L, Haggstrom L, Enfors S. 1994. Modeling of high cell density fed batch cultivation. FEMS Microbiol Rev 14: 39-44.
7. Davey HM, Kell DB. 1996. Flow cytometry and cell sorting of heterogeneous microbial populations: The importance of single-cell analyses. Microbiol Rev 60:641.
8. Elsworth R, Miller G, Whitaker A, Kitching D, Sayer P. 1968. Production of E. coli as a source of nucleic acids. J Appl Chem 17:157-166.
9. Forberg C, Haggstrom L. 1987. Effects of cultural conditions on the production of phenylalanine from a plasmid-harboring Escherichia coli strain. Appl Microbiol Biotechnol 26:136-140.
10. Hercher M, Mueller W, Shapiro HM. 1979. Detection and discrimination of individual viruses by flow cytometry. J Histochem Cytochem 27: 350-352.
11. Hewitt CJ, Boon L, McFarlane CM, Nienow AW. 1998a. An application of bacterial flow cytometry - Studies on the fluid mechanical stress of E. coli. Cytometry (Suppl.) 9:55.
12. Hewitt CJ, Boon LA, McFarlane CM, Nienow AW. 1998b. The use of flow cytometry 10 study the impact of fluid mechanical stress on E. coli during continuous cultivation in an agitated bioreactor. Biotechnol Bioeng 29:612-620.
13. Lee SY. 1996. High cell-density culture of E. coli. Trends Biotechnol 14:98-105.
14. Meyer HP, Leist C, Fiechter A. 1984. Acetate formation in continuous culture of Escherichia coli K12 D1 on defined and complex media. J Biotechnol 1:355-358.
15. Midgley M. 1987. An efflux system for cationic dyes and related compounds in E. coli. Microbiol Sci 4:125-128.
16. Minihane BJ, Brown DE. 1986. Fed-batch culture technology. Biotechnol Adv 4:207-218.
17. Nebe-von Caron G, Stephens P, Badley RA. 1998. Assessment of bacterial viability status by flow cytometry and single cell sorting. J Appl Microbiol, in press.
18. Nebe-von Caron G, Badley RA. 1995. Viability assessment of bacteria in mixed populations using flow-cytometry. J Microsc (Oxford) 179: 55-66.
19. Obernesser MS, Socransky SS, Stashenko P. 1990. Limit of resolution of flow cytometry for the detection of selected bacterial species. J Dental Res 69:1592-1598.
20. Phillips AP, Martin KL. 1988. Limitations of flow cytometry for the specific detection of bacteria in mixed populations. J Immunol Methods 106:109-117.
21. Pore RS. 1994. Antibiotic susceptibility testing by flow-cytometry. J Antimicrob Chemother 34:613-627.
22. Porter J, Deere D, Pickup R, Edwards C. 1996. Fluorescent probes and flow cytometry: New insights into environmental bacteriology. Cytometry 23:91-96.
23. Riesenberg D, Schulz, V. 1991. High cell density cultivation of E. coli at controlled specific growth rates. J Biotechnol 20:17-28.
24. Steen HB, Skarstad K, Boye E. 1986. Flow cytometry of bacteria: Cell cycle kinetics and effects of antibiotics. Ann NY Acad Sci 468: 329-338.
25. Strandberg L, Andersson L, Enfors S. 1994. The use of fed batch cultivation for achieving high cell densities in the production of a recombinant protein in Escherichia coli. FEMS Microbiol Rev 14:53-56.
26. Zimmerman R, Iturriaga R, Becker-Birck, J. 1978. Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl Environ Microbiol 36:926-935.