Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation

Biotechnology and Bioengineering

Volumn 92, Issue 1, 2005, Pages 69-78

  Looser, V ^a   Hammes, F ^b   Keller, M ^a   Berney, M ^b   Kovar, K ^a   Egli, Thomas ^b  

^a ZURICH UNIVERSITY OF APPLIED SCIENCES   (Switzerland)

^b SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

Author keywords

E. coli; Ethidium bromide; Fedbatch cultivation; Flow cytometry; Physiological state; Propidium iodide; Recombinant membrane protein; Xylene monooxygenase

Indexed keywords

CELL CULTURE; CYTOLOGY; ENZYMES; PHYSIOLOGY;

ETHIDIUM BROMIDE; FEDBATCH CULTIVATION; FLOW CYTOMETRY; PHYSIOLOGICAL STATE;

ESCHERICHIA COLI;

ACETIC ACID; BACTERIAL PROTEIN; BROMIDE; CARBON; CARBON DIOXIDE; ETHIDIUM BROMIDE; GLUCOSE; MEMBRANE PROTEIN; PROPIDIUM IODIDE; RECOMBINANT PROTEIN; UNSPECIFIC MONOOXYGENASE; XYLENE;

ARTICLE; BACTERIUM DETECTION; CELL DAMAGE; CELL DENSITY; ESCHERICHIA COLI; FLOW CYTOMETRY; NONHUMAN; PHYSIOLOGY; PLASMID; PROTEIN EXPRESSION; PROTEIN PROCESSING;

ACETATES; BIOREACTORS; CARBON; CELL MEMBRANE; CULTURE MEDIA; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; ETHIDIUM; FERMENTATION; FLOW CYTOMETRY; FLUORESCENT DYES; GLUCOSE; INDUSTRIAL MICROBIOLOGY; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; OXYGENASES; PROPIDIUM; RECOMBINANT PROTEINS; TIME FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 27844523810     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20575     Document Type: Article

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