Overexpression of ovine leptin in Pichia pastoris: Physiological yeast response to leptin production and characterization of the recombinant hormone

Yeast

Volumn 21, Issue 3, 2004, Pages 249-263

  Laborde, C ^a   Chemardin, P ^a   Bigey, F ^a   Combarnous, Y ^b   Moulin, G ^a   Boze, H ^a  

^a CEMAGREF   (France)

^b CNRS   (France)

Author keywords

Cellular stress; Cellular viability; Fed batch culture; Leptin; Pichia pastoris; Proteolysis; Secretion

Indexed keywords

LEPTIN; MATING HORMONE ALPHA FACTOR; RECOMBINANT HORMONE; TYROSINE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DRUG ACTIVITY; DRUG ISOLATION; DRUG MANUFACTURE; DRUG PURIFICATION; FED BATCH CULTURE; FUNGAL BIOMASS; FUNGUS CULTURE; GENE CONTROL; GENE INDUCTION; GENE NUMBER; GENE OVEREXPRESSION; GENE VECTOR; GROWTH RATE; IN VITRO STUDY; MOLECULAR CLONING; MOLECULAR WEIGHT; MORTALITY; MOUSE; NONHUMAN; PH; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; SHEEP; STRESS;

ANIMALS; BIOREACTORS; BLOTTING, SOUTHERN; BLOTTING, WESTERN; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; DNA; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE DOSAGE; INDUSTRIAL MICROBIOLOGY; LEPTIN; MOLECULAR WEIGHT; PICHIA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SHEEP; TRANSFORMATION, GENETIC;

OVIS; PICHIA; PICHIA PASTORIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 1342290473     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.1073     Document Type: Article

References (37)

1. Bentley WE, Mirjalili N, Andersen DC, et al. 1990. The principal factor in the 'metabolic burden' associated with recombinant bacteria. Biotechnol Bioeng 35: 668-681.
2. Boze H, Laborde C, Chemardin P, et al. 2001. High-level secretory production of recombinant porcine follicle-stimulating hormone by Pichia pastoris. Proc Biochem 36: 907-913.
3. Brierley RA, Bussieau C, Kosson R, Melton A and Siegel RS. 1990. Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: bovine lysozyme. Biochem Eng VI. Ann NY Acad Sci 589: 350-363.
4. Broekaert D, Eyckerman S, Lavens D, et al. 2002. Comparison of leptin- and interleukin-6-regulated expression of the rPAP gene family: evidence for differencial co-regulatory signals. Eur Cytokine Netw 13: 78-85.
5. Clare JJ, Rayment FB, Ballantine SP, Sreekrishna K, Romano MA. 1991a. High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Biotechnology 9: 455-460.
6. Clare JJ, Romano MA, Rayment FR, et al. 1991b. Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105: 205-212.
7. Cregg JM, Barringer KJ, Hessler AY, Madden KR. 1985. Pichia pastoris as a host system for transformations. Mol Cell Biol 5: 3376-3385.
8. Cregg JM, Tschopp JF, Stilman C, et al. 1987. High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast, Pichia pastoris. Biotechnology 5: 479-485.
9. Cregg JM, Vedvick TS, Raschke WC. 1993. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology 11: 905-910.
10. Gertler A, Simmons J, Keisler DH. 1998. Large-scale preparation of biologically active recombinant ovine obese protein (leptin). FEBS Lett 422: 137-140.
11. Fawzi AB, Zhang HP, Van Heek M, Graziano MP. 1996. Purification of milligram quantities of human leptin from recombinant E. coli. Horm Metab Res 28: 694-697.
12. Guisez Y, Fache I, Campfield LA, et al. 1998. Efficient secretion of biologically active recombinant OB protein (leptin) in Escherichia coli, purification from the periplasm and characterization. Protein Expr Purif 12: 249-258.
13. Halaas JL, Gajiwala KS, Maffei M, et al. 1995. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269: 543-546.
14. Henry A, Masters CL, Beyreuther K, Cappai R. 1997. Expression of human amyloid precursor protein ectodomains in Pichia pastoris: analysis of culture conditions, purification, and characterization. Protein Expr Purif 10: 283-291.
15. Hohenblum H, Maurer M, Mattanovich D. 2002. Dynamics of growth, viability and product formation in recombinant Pichia pastoris fermentations. Second International Conference on Recombinant Protein Production with Prokaryotic and Eukaryotic cells. A Comparative View on Host Physiology, Cernobbio, Italy.
16. Jeong KJ, Lee SY. 1999. High-level production of human leptin by fed-batch cultivation of recombinant Escherichia coli and its purification. Appl Environ Microbiol 65: 3027-3032.
17. Klein C, de La Motte Guery F, Gautier F, et al. 1998. High-level secretion of a wheat lipid transfer protein in Pichia pastoris. Protein Expr Purif 13: 73-82.
18. King ML, Schisler DO, Ruocco JJ. 1981. Epifluorescent method for detection of non-viable yeast. J Am Soc Brew Chem 39: 52-54.
19. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227: 680-685.
20. Lin HY, Hanschkle R, Nicklisch S, et al. 2001. Cellular responses to strong overexpression of recombinant genes in Escherichia coli. DNA relaxation and cell death after induction of α-glucosidase. In Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology. Kluwer Academic: Dordrecht; 55-73.
21. Neubauer P, Winter J. 2001. Expression and fermentation strategies for recombinant protein production in Escherichia coli. In Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology. Kluwer Academic: Dordrecht; 195-258.
22. Park JH, Lee HH, Na SY, et al. 2001. Recombinant expression of biologically active rat leptin in Escherichia coli. Protein Expr Purif 22(1): 60-69.
23. Pelleymounter MA, Cullen MJ, Baker MB, et al. 1995. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269: 540-543.
24. Scorer CA, Buckholz RG, Clare JJ, Romanos MA. 1993. The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene 136: 111-119.
25. Sorensen MA, Pedersen S. 1991. Absolute in vivo translation rates of individual codons in E. coli: the two glutamic acid codons GAA and GAG are translated with a threefold difference in rate. J Mol Biol 222: 265-280.
26. Southern EM. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503-517.
27. Sreekrishna K, Nelles L, Potenz R, et al. 1989. High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris. Biochemistry 28: 4117-4125.
28. Stickland LH. 1951. The determination of small quantities of bacteria by means of Biuret reaction. J Gen Microbiol 5: 698.
29. Thill GP, Davis GR, Stillman C, et al. 1990. Positive and negative effects on multi-copy integrated expression vectors on protein expression in Pichia pastoris. Proceedings of the Sixth International Symposium on Genetics of Industrial Microorganisms, Strasbourg, France. Société Française de Microbiologie: Paris; 477-490.
30. Tschopp JF, Brust PF, Cregg JM, Stillman CA, Gingeras TR. 1987. Expession of lacZ gene from two methanol-regulated promoter in Pichia pastoris. Nucleic Acids Res 15: 3859-3876.
31. Tsujikawa M, Okabayashi K, Morita M, Tanabe T. 1996. Secretion of a variant of human single-chain urokinase-type plasminogen activator without an N-glycosylation site in the methylotrophic yeast, Pichia pastoris and characterisation of the secreted product. Yeast 12: 541-553.
32. Varnerin JP, Smith T, Rosenblum CI, et al. 1998. Production of leptin in Escherichia coli : a comparison methods. Protein Expr Purif 14: 335-342.
33. Werten MWT, Van den Bosch TJ, Wind RD, et al. 1999. High-yield secretion of recombinant gelatins by Pichia pastoris. Yeast 15(11): 1087-1096.
34. Zahn K. 1996. Overexpression of an mRNA dependent on rare codons inhibits protein synthesis and cell growth. J Bacteriol 178: 2926-2933.
35. Zamorano PL, De Sevilla L, Mahesh VB, Brann DW. 1997. Production and refolding of recombinant leptin. Biotechniques 23: 800-802.
36. Zhang Y, Proenca R, Maffei M, et al. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 372: 425-432.
37. Zhang F, Basinski MB, Beals JM, et al. 1997. Crystal structure of the obese protein leptin-E100. Nature 387: 206-209.