Recombinant cold-adapted trypsin I from Atlantic cod - Expression, purification, and identification

Protein Expression and Purification

Volumn 33, Issue 1, 2004, Pages 110-122

  Jónsdóttir, Gudrún ^a   Bjarnason, Jón Bragi ^a   Gudmundsdóttir, Ágústa ^a  

^a UNIVERSITY OF ICELAND   (Iceland)

Author keywords

Cold adapted; Expression; Purification; Serine protease; Trypsin

Indexed keywords

BOVINAE; ESCHERICHIA COLI; GADUS MORHUA; VECTORS;

EID: 0347093698     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.09.012     Document Type: Article

References (33)

1. B. Ásgeirsson, J. Fox, J.B. Bjarnason, Purification and characterization of trypsin from the poikilotherm Gadus morhua, Eur. J. Biochem. 180 (1989) 85-94.
2. A.O. Smalås, E.S. Heimstad, A. Hordvik, N.P. Willassen, R. Male, Cold adaptation of enzymes: structural comparison between salmon and bovine trypsins, Proteins 20 (1994) 149-166.
3. Á Gudmundsdóttir, E. Gudmundsdóttir, S. Óskarsson, J.B. Bjarnason, A.K. Eakin, C.S. Craik, Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen, Eur. J. Biochem. 217 (1993) 1091-1097.
4. H-K. Schrøder-Leiros, N.P. Willassen, A.O. Smalås, Structural comparison of psychrophilic and mesophilic trypsins - elucidating the molecular basis of cold adaptation, Eur. J. Biochem. 267 (2000) 1039-1049.
5. G. Feller, Molecular adaptations to cold in psychrophilic enzymes, Cell. Mol. Life Sci. 60 (2003) 648-662.
6. P. Závodsky, J. Kardos, Á. Svingor, G.A. Petsko, Adjustment of conformational flexibility is a key event in the thermal adaptation of proteins, Proc. Natl. Acad. Sci. USA 95 (1998) 7406-7411.
7. A.O. Smalås, H-K. Schrøder-Leiros, V. Os, N.P. Willassen, Cold-adapted enzymes, Biotechnol. Annu. Rev. 6 (2000) 1-57.
8. N. Aghajari, F. Van Petegem, V. Villeret, J.P. Chessa, C. Gerday, R. Haser, J. Van Beeumen, Crystal structures of a psychrophilic metalloprotease reveal new insights into catalysis by cold-adapted proteases, Proteins 50 (2003) 636-647.
9. B. Shoichet, W.A. Baase, R. Kuroki, B.W. Matthews, A relationship between protein stability and protein function, Proc. Natl. Acad. Sci. USA 92 (1995) 452-456.
10. S. D'Amico, J.C. Marx, C. Gerday, G. Feller, Activity-stability relationships in extremophilic enzymes, J. Biol. Chem. 278 (2003) 7891-7896.
11. K. Miyazaki, P.L. Wintrode, R.A. Greyling, D.N. Rubingh, F.H. Arnold, Direct evolution study of temperature adaptation in a psychrophilic enzyme, J. Mol. Biol. 297 (2000) 1015-1026.
12. M. Macouzet, B.K. Simpson, B.H. Lee, Cloning of fish enzymes and other fish protein genes, Crit. Rev. Biotechnol. 19 (1999) 179-196.
13. L. Helgadóttir, Trypsin I from Atlantic cod: purification, specificity, stability and autolysis, M.Sc. Thesis, Department of Chemistry, Faculty of Sciences, University of Iceland, Reykjavík, Iceland (2002).
14. L. Birolo, M.L. Tutino, B. Fontanella, C. Gerday, K. Mainolfi, S. Pascarella, G. Sannia, F. Vinci, G. Marino, Aspartate aminotransferase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC 125, cloning, expression, properties and molecular modelling, Eur. J. Biochem. 267 (2002) 2790-2802.
15. G. Feller, O. Le Bussy, C. Gerday, Expression of psychrophilic genes in mesophilic hosts: assessment of the folding state of a recombinant alpha-amylase, Appl. Environ. Microbiol. 64 (2000) 1163-1165.
16. J. Arnórsdóttir, R.B. Smáradóttir, Ó.B. Magnússon, S.H. Thorbjarnardóttir, G. Eggertsson, M.M. Kristjánsson, Characterization of a cloned subtilisin-like serine protease from a psychrotrophic Vibrio species, Eur. J. Biochem. 269 (2002) 5536-5546.
17. J.A. Irwin, S.V. Lynch, S. Coughlan, P.J. Baker, H.M. Gudmundsson, G.A. Alfredsson, D.W. Rice, P.C. Engel, Alanine dehydrogenase from the psychrophilic bacterium strain PA-43: overexpression, molecular characterization, and sequence analysis, Extremophiles 7 (2003) 135-143.
18. O. Lanes, I. Leiros, A.O. Smalås, N.P. Willassen, Identification, cloning and expression of uracil-DNA glycosylase from Atlantic cod (Gadus morhua): characterization and homology modelling of the cold-adapted catalytic domain, Extremophiles 1 (2002) 73-86.
19. S. Kristjánsdóttir, Á. Gudmundsdóttir, Propeptide dependent activation of the Antarctic krill euphauserase precursor produced in yeast, Eur. J. Biochem. 267 (2000) 2632-2639.
20. R. Spilliaert, Á. Gudmundsdóttir, Atlantic cod trypsin Y - member of a novel trypsin group, Marine Biotechnol. 1 (1999) 598-607.
21. J.B. Bjarnason, B. Ásgeirsson, M.M. Kristjánsson, Á. Gudmundsdóttir, J.W. Fox, J.F. Chlebowski, C.S. Craik, Characteristics, protein engineering and applications of psychrophilic marine proteinases from atlantic cod, in: W.J.J. van den Tweel, A. Harder, R.M. Buitelaar (Eds.), Stability and Stabilization of Enzymes, Proceedings of an International Symposium held in Maastricht, The Netherlands, Elsevier Science Publishers, The Netherlands, 1993, pp. 205-214.
22. J.B. Bjarnason, B. Benediktsson, Protein hydrolysates produced with the use of marine proteases, Patent: PCT, WO 01/28353 A2 (26, April 2001).
23. J.B. Bjarnason, Fish serine proteases and their pharmaceutical and cosmetic use, Patent: PCT, WO 00/78332 A2 (28, December 2000).
24. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. More, J.G. Seidman, J.A. Smith, K. Struhl, Current Protocols in Molecular Biology, Greene Publishing Associates and Wiley-Interscience, NY, 1989.
25. A. Light, H. Janska, Enterokinase (enteropeptidase): comparative aspects, Trends Biochem. Sci. 14 (1989) 110-112.
26. E.R. LaVallie, E.A. DiBlasio, S. Kovacic, K.L. Grant, P.F. Schendel, J.M. McCoy, A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm, Bio/Technology 11 (1993) 187-193.
27. B. Ásgeirsson, J.B. Bjarnason, Structural and kinetic properties of chymotrypsin from Atlantic cod (Gadus morhua), comparison with bovine chymotrypsin, Comp. Biochem. Physiol. 2 (1991) 327-335.
28. M.M. Kristjánsson, S. Gudmundsdóttir, J.W. Fox, J.B. Bjarnason, Characterization of a collagenolytic serine proteinase from Atlantic cod (Gadus morhua), Comp. Biochem. Physiol. 4 (1995) 707-717.
29. D.C. Benjamin, S. Kristjánsdóttir, Á. Gudmundsdóttir, Increasing the thermal stability of euphauserase: a cold active and multifunctional serine protease from Antarctic krill, Eur. J. Biochem. 268 (2001) 127-131.
30. E. Narinx, E. Baise, C. Gerday, Subtilisin from psychrophilic Antarctic bacteria: characterization and site-directed mutagenesis of residues possibly involved in the adaptation to cold, Protein Eng. 11 (1997) 1271-1279.
31. C. Gerday, M. Aittaleb, M. Bentahir, E. Baise, S. Genicot, C. Gerday, Cold-adapted enzymes: from fundamentals to biotechnology, Trends Biotechnol. 18 (2000) 103-107.
32. S. Taguchi, A. Ozaki, T. Nonaka, Y. Mitsui, H. Momose, A cold-adapted protease engineered by experimental evolution system, J. Biochem. (Tokyo) 126 (1999) 689-693.
33. U. Gerike, M.J. Danson, D.W. Hough, Cold-active citrate synthase: mutagenesis of active-site residues, Protein Eng. 14 (2001) 655-661.