Biotechnological applications of microbial proteomes

Journal of Biotechnology

Volumn 145, Issue 4, 2010, Pages 341-349

  Han, Mee Jung ^a   Lee, Sang Yup ^b   Koh, Seung Tae ^a   Noh, Sang Gyun ^a   Han, Won Hee ^a  

^a Dongyang University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Biotechnology; Microbial proteomics; Microorganisms; Multi omics; Two dimensional gel electrophoresis

Indexed keywords

BIOPROCESSES; BIOTECHNOLOGICAL APPLICATIONS; BIOTECHNOLOGY INDUSTRY; FUSION PARTNERS; IDENTIFICATION OF PROTEINS; MICROBIAL PROTEOMICS; MULTI-OMICS; POTENTIAL APPLICATIONS; PROTEOME DATABASE; PROTEOMES; PROTEOMIC APPROACHES; PROTEOMIC TECHNIQUES; PROTEOMIC TECHNOLOGIES; PROTEOMICS; REGULATORY NETWORK; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

BIOTECHNOLOGY; ELECTROPHORESIS; GELS; MICROORGANISMS; MOLECULAR BIOLOGY; TARGETS; TWO DIMENSIONAL;

PROTEINS;

CELL PROTEIN; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MEMBRANE PROTEIN; MICROORGANISM PROTEIN; OUTER MEMBRANE PROTEIN; PROTEINASE; RECOMBINANT PROTEIN; REGULATOR PROTEIN; THIOREDOXIN;

AMINO ACID SEQUENCE; BIOTECHNOLOGY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEIN SYNTHESIS; PROTEIN TARGETING; PROTEOMICS; RECOMBINANT DNA TECHNOLOGY; REVIEW; STRESS;

BACTERIA; BIOTECHNOLOGY; PROTEOME; PROTEOMICS;

EID: 77049111758     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.12.018     Document Type: Review

References (99)

1. Aldor I.S., Krawitz D.C., Forrest W., Chen C., Nishihara J.C., Joly J.C., Champion K.M. Proteomic profiling of recombinant Escherichia coli in high-cell-density fermentations for improved production of an antibody fragment biopharmaceutical. Appl. Environ. Microbiol. 2005, 71:1717-1728.
2. Allen S.P., Polazzi J.O., Gierse J.K., Easton A.M. Two novel heat shock genes encoding proteins produced in response to heterologous protein expression in Escherichia coli. J. Bacteriol. 1992, 174:6938-6947.
3. Baek J.H., Han M.-J., Lee S.Y., Yoo J.S. Transcriptome and proteome analyses of adaptive responses to methyl methanesulfonate in Escherichia coli K-12 and ada mutant strains. BMC Microbiol. 2009, 9:186.
4. Baneyx F., Palumbo J.L. Improving heterologous protein folding via molecular chaperone and foldase co-expression. Methods Mol. Biol. 2003, 205:171-197.
5. Blobel G., Sabatini D.D. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. I. Location of the polypeptides within ribosomes. J. Cell Biol. 1970, 45:130-145.
6. Brown W.C., Campbell J.L. A new cloning vector and expression strategy for genes encoding proteins toxic to Escherichia coli. Gene 1993, 127:99-103.
7. Bukau B., Weissman J., Horwich A. Molecular chaperones and protein quality control. Cell 2006, 125:443-451.
8. Bumann D., Aksu S., Wendland M., Janek K., Zimny-Arndt U., Sabarth N., Meyer T.F., Jungblut P.R. Proteome analysis of secreted proteins of the gastric pathogen Helicobacter pylori. Infect. Immun. 2002, 70:3396-3403.
9. Chakraborty A., Regnier F.E. Global internal standard technology for comparative proteomics. J. Chromatogr. A 2002, 949:173-184.
10. Chang H.J., Sheu S.Y., Lo S.J. Expression of foreign antigens on the surface of Escherichia coli by fusion to the outer membrane protein traT. J. Biomed. Sci. 1999, 6:64-70.
11. Choi J.H., Choi J.I., Lee S.Y. Display of proteins on the surface of Escherichia coli by C-terminal deletion fusion to the Salmonella typhimurium OmpC. J. Microbiol. Biotechnol. 2005, 15:141-146.
12. Doherty A.J., Connolly B.A., Worrall A.F. Overproduction of the toxic protein, bovine pancreatic DNaseI, in Escherichia coli using a tightly controlled T7-promoter-based vector. Gene 1993, 136:337-340.
13. Duy N.V., Mäder U., Tran N.P., Cavin J.F., le Tam T., Albrecht D., Hecker M., Antelmann H. The proteome and transcriptome analysis of Bacillus subtilis in response to salicylic acid. Proteomics 2007, 7:698-710.
14. Emslie K.R., Miller J.M., Slade M.B., Dormitzer P.R., Greenberg H.B., Williams K.L. Expression of the rotavirus SA11 protein VP7 in the simple eukaryote Dictyostelium discoideum. J. Virol. 1995, 69:1747-1754.
15. Faraldo-Gómez J.D., Sansom M.S. Acquisition of siderophores in Gram-negative bacteria. Nat. Rev. Mol. Cell Biol. 2003, 4:105-116.
16. Fountoulakis M., Gasser R. Proteomic analysis of the cell envelope fraction of Escherichia coli. Amino Acids 2003, 24:19-41.
17. Goloubinoff P., Gatenby A.A., Lorimer G.H. GroE heat-shock proteins promote assembly of foreign prokaryotic ribulose bisphosphate carboxylase oligomers in Escherichia coli. Nature 1989, 337:44-47.
18. Han M.-J., Lee S.Y. Proteome profiling and its use in metabolic and cellular engineering. Proteomics 2003, 3:2317-2324.
19. Han M.-J., Lee S.Y. The Escherichia coli proteome: past, present, and future prospects. Microbiol. Mol. Biol. Rev. 2006, 70:362-439.
20. Han M.-J., Jeong K.J., Yoo J.S., Lee S.Y. Engineering Escherichia coli for increased productivity of serine-rich proteins based on proteome profiling. Appl. Environ. Microbiol. 2003, 69:5772-5781.
21. Han M.-J., Park S.J., Park T.J., Lee S.Y. Roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli. Biotechnol. Bioeng. 2004, 88:426-436.
22. Han M.-J., Lee J.W., Lee S.Y. Enhanced proteome profiling by inhibiting proteolysis with small heat shock proteins. J. Proteome Res. 2005, 4:2429-2434.
23. Han K.Y., Seo H.S., Song J.A., Ahn K.Y., Park J.S., Lee J. Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression. Biochim. Biophys. Acta 2007, 1774:1536-1543.
24. Han K.Y., Song J.A., Ahn K.Y., Park J.S., Seo H.S., Lee J. Enhanced solubility of heterologous proteins by fusion expression using stress-induced Escherichia coli protein, Tsf. FEMS Microbiol. Lett. 2007, 274:132-138.
25. Han K.Y., Song J.A., Ahn K.Y., Park J.S., Seo H.S., Lee J. Solubilization of aggregation-prone heterologous proteins by covalent fusion of stress-responsive Escherichia coli protein, SlyD. Protein Eng. Des. Sel. 2007, 20:543-549.
26. Han K.Y., Park J.S., Seo H.S., Ahn K.Y., Lee J. Multiple stressor-induced proteome responses of Escherichia coli BL21(DE3). J. Proteome Res. 2008, 7:1891-1903.
27. Han M.-J., Lee J.W., Lee S.Y., Yoo J.S. Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein production. J. Biomed. Biotechnol. 2008, 735101.
28. Hirose I., Sano K., Shioda I., Kumano M., Nakamura K., Yamane K. Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study. Microbiology 2000, 146:65-75.
29. Hoffmann F., Rinas U. On-line estimation of the metabolic burden resulting from synthesis of plasmid-encoded and heat-shock proteins by monitoring respiratory energy generation. Biotechnol. Bioeng. 2001, 76:333-340.
30. Hoffmann F., Weber J., Rinas U. Metabolic adaptation of Escherichia coli during temperature induced recombinant protein synthesis: 1. Readjustment of metabolic enzyme synthesis. Biotechnol. Bioeng. 2002, 80:313-319.
31. Höper D., Bernhardt J., Hecker M. Salt stress adaptation of Bacillus subtilis: a physiological proteomics approach. Proteomics 2006, 6(March (5)):1550-1562.
32. Huber L.A., Pfaller K., Vietor I. Organelle proteomics: implications for subcellular fractionation in proteomics. Circ. Res. 2003, 92:962-968.
33. Ideker T., Thorsson V., Ranish J.A., Christmas R., Buhler J., Eng J.K., Bumgarner R., Goodlett D.R., Aebersold R., Hood L. Integrated genomic and proteomic analyses of a systematically perturbed metabolic network. Science 2001, 292:929-934.
34. Jeong K.J., Lee S.Y. Excretion of human beta-endorphin into culture medium by using outer membrane protein F as a fusion partner in recombinant Escherichia coli. Appl. Environ. Microbiol. 2002, 68:4979-4985.
35. Jones R.C., Deck J., Edmondson R.D., Hart M.E. Relative quantitative comparisons of the extracellular protein profiles of Staphylococcus aureus UAMS-1 and its sarA, agr, and sarA agr regulatory mutants using one-dimensional polyacrylamide gel electrophoresis and nanocapillary liquid chromatography coupled with tandem mass spectrometry. J. Bacteriol. 2008, 190:5265-5278.
36. Josic D., Kovač S. Application of proteomics in biotechnology-microbial proteomics. Biotechnol. J. 2008, 3:496-509.
37. Joyce A.R., Palsson B.Ø. The model organism as a system: integrating 'omics' data sets. Nat. Rev. Mol. Cell Biol. 2006, 7:198-210.
38. Jürgen B., Lin H.Y., Riemschneider S., Scharf C., Neubauer P., Schmid R., Hecker M., Schweder T. Monitoring of genes that respond to overproduction of an insoluble recombinant protein in Escherichia coli glucose-limited fed-batch fermentations. Biotechnol. Bioeng. 2000, 70:217-224.
39. Kolaj O., Spada S., Robin S., Wall J.G. Use of folding modulators to improve heterologous protein production in Escherichia coli. Microb. Cell Fact. 2009, 8:9.
40. Kuczyńska-Wiśnik D., Zurawa-Janicka D., Narkiewicz J., Kwiatkowska J., Lipińska B., Laskowska E. Escherichia coli small heat shock proteins IbpA/B enhance activity of enzymes sequestered in inclusion bodies. Acta Biochim. Pol. 2004, 51:925-931.
41. Lee S.Y. High cell-density culture of Escherichia coli. Trends Biotechnol. 1996, 14:98-105.
42. Lee P.S., Lee K.H. Engineering HlyA hypersecretion in Escherichia coli based on proteomic and microarray analyses. Biotechnol. Bioeng. 2005, 89:195-205.
43. Lee J.H., Lee D.E., Lee B.U., Kim H.S. Global analyses of transcriptomes and proteomes of a parent strain and an l-threonine-overproducing mutant strain. J. Bacteriol. 2003, 185:5442-5451.
44. Lee S.Y., Choi J.H., Xu Z. Microbial cell-surface display. Trends Biotechnol. 2003, 21:45-52.
45. Lee S.H., Choi J.I., Park S.J., Lee S.Y., Park B.C. Display of bacterial lipase on the Escherichia coli cell surface by using FadL as an anchoring motif and use of the enzyme in enantioselective biocatalysis. Appl. Environ. Microbiol. 2004, 70:5074-5080.
46. Lee S.H., Choi J.I., Han M.J., Choi J.H., Lee S.Y. Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its application to enantioselective resolution in organic solvent. Biotechnol. Bioeng. 2005, 90:223-230.
47. Lee S.Y., Lee D.Y., Kim T.Y. Systems biotechnology for strain improvement. Trends Biotechnol. 2005, 23:349-358.
48. Lee K.H., Park J.H., Kim T.Y., Kim H.U., Lee S.Y. Systems metabolic engineering of Escherichia coli for l-threonine production. Mol. Syst. Biol. 2007, 3:149.
49. Len A.C., Cordwell S.J., Harty D.W., Jacques N.A. Cellular and extracellular proteome analysis of Streptococcus mutans grown in a chemostat. Proteomics 2003, 3:627-646.
50. LeThanh H., Neubauer P., Hoffmann F. The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies. Microb. Cell Fact. 2005, 4:6.
51. Lin X.M., Li H., Wang C., Peng X.X. Proteomic analysis of nalidixic acid resistance in Escherichia coli: identification and functional characterization of OM proteins. J. Proteome Res. 2008, 7:2399-2405.
52. Lopez-Campistrous A., Semchuk P., Burke L., Palmer-Stone T., Brokx S.J., Broderick G., Bottorff D., Bolch S., Weiner J.H., Ellison M.J. Localization, annotation and comparison of the Escherichia coli K-12 proteome under two states of growth. Mol. Cell. Proteomics 2005, 4:1205-1209.
53. Majander K., Anton L., Antikainen J., Lång H., Brummer M., Korhonen T.K., Westerlund-Wikström B. Extracellular secretion of polypeptides using a modified Escherichia coli flagellar secretion apparatus. Nat. Biotechnol. 2005, 23:475-481.
54. Majander K., Korhonen T.K., Westerlund-Wikström B. Simultaneous display of multiple foreign peptides in the FliD capping and FliC filament proteins of the Escherichia coli flagellum. Appl. Environ. Microbiol. 2005, 71:4263-4268.
55. Makrides S.C. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 1996, 60:512-538.
56. Masuda T., Saito N., Tomita M., Ishihama Y. Unbiased quantitation of Escherichia coli membrane proteome using phase-transfer surfactants. Mol. Cell. Proteomics 2009, 10.1074/mcp.M900240-MCP200.
57. McBroom A.J., Kuehn M.J. Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response. Mol. Microbiol. 2007, 63:545-558.
58. Medina M.L., Kiernan U.A., Francisco W.A. Proteomic analysis of rutin-induced secreted proteins from Aspergillus flavus. Fungal Genet. Biol. 2004, 41:327-335.
59. Molloy M.P., Herbert B.R., Slade M.B., Rabilloud T., Nouwens A.S., Williams K.L., Gooley A.A. Proteomic analysis of the Escherichia coli outer membrane. Eur. J. Biochem. 2000, 267:2871-2881.
60. Murby M., Cedergren L., Nilsson J., Nygren P.A., Hammarberg B., Nilsson B., Enfors S.O., Uhlén M. Stabilization of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy. Biotechnol. Appl. Biochem. 1991, 14:336-346.
61. Nandakumar M.P., Cheung A., Marten M.R. Proteomic analysis of extracellular proteins from Escherichia coli W3110. J. Proteome Res. 2006, 5:1155-1161.
62. Neidhardt F.C., VanBogelen R.A. Proteomic analysis of bacterial stress responses. Bacterial Stress Responses 2000, 445-452. ASM Press, Washington, DC. G. Storz, H. Hennecke (Eds.).
63. Nguyen V.D., Wolf C., Mäder U., Lalk M., Langer P., Lindequist U., Hecker M., Antelmann H. Transcriptome and proteome analyses in response to 2-methylhydroquinone and 6-brom-2-vinyl-chroman-4-on reveal different degradation systems involved in the catabolism of aromatic compounds in Bacillus subtilis. Proteomics 2007, 7:1391-1408.
64. Oda K., Kakizono D., Yamada O., Iefuji H., Akita O., Iwashita K. Proteomic analysis of extracellular proteins from Aspergillus oryzae grown under submerged and solid-state culture conditions. Appl. Environ. Microbiol. 2006, 72:3448-3457.
65. Park J.H., Lee K.H., Kim T.Y., Lee S.Y. Metabolic engineering of Escherichia coli for the production of l-valine based on transcriptome analysis and in silico gene knockout simulation. Proc. Natl. Acad. Sci. U.S.A. 2007, 104:7797-7802.
66. Park J.S., Han K.Y., Song J.A., Ahn K.Y., Seo H.S., Lee J. Escherichia coli malate dehydrogenase, a novel solubility enhancer for heterologous proteins synthesized in Escherichia coli. Biotechnol. Lett. 2007, 29:1513-1518.
67. Park J.S., Han K.Y., Lee J.H., Song J.A., Ahn K.Y., Seo H.S., Sim S.J., Kim S.W., Lee J. Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coli protein, RpoS. BMC Biotechnol. 2008, 8:15.
68. Qian Z.G., Xia X.X., Choi J.H., Lee S.Y. Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli. Biotechnol. Bioeng. 2008, 101:587-601.
69. Raman B., Nandakumar M.P., Muthuvijayan V., Marten M.R. Proteome analysis to assess physiological changes in Escherichia coli grown under glucose-limited fed-batch conditions. Biotechnol. Bioeng. 2005, 92:384-392.
70. Ramérez D.M., Bentley W.E. Enhancement of recombinant protein synthesis and stability via coordinated amino acid addition. Biotechnol. Bioeng. 1993, 41:557-565.
71. Rosen R., Ron E.Z. Proteome analysis in the study of the bacterial heat-shock response. Mass Spectrom. Rev. 2002, 21:244-265.
72. Ross P.L., Huang Y.N., Marchese J.N., Williamson B., Parker K., Hattan S., Khainovski N., Pillai S., Dey S., Daniels S., Purkayastha S., Juhasz P., Martin S., Bartlet-Jones M., He F., Jacobson A., Pappin D.J. Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol. Cell. Proteomics 2004, 3:1154-1169.
73. Sabatini D.D., Blobel G. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes. J. Cell Biol. 1970, 45:146-157.
74. Sato M., Machida K., Arikado E., Saito H., Kakegawa T., Kobayashi H. Expression of outer membrane proteins in Escherichia coli growing at acid pH. Appl. Environ. Microbiol. 2000, 66:943-947.
75. Soufi B., Kelstrup C.D., Stoehr G., Fröhlich F., Walther T.C., Olsen J.V. Global analysis of the yeast osmotic stress response by quantitative proteomics. Mol. Biosyst. 2009, 5:1337-1346.
76. Sousa C., Kotrba P., Ruml T., Cebolla A., De Lorenzo V. Metalloadsorption by Escherichia coli cells displaying yeast and mammalian metallothioneins anchored to the outer membrane protein LamB. J. Bacteriol. 1998, 180:2280-2284.
77. Tan Y.P., Lin Q., Wang X.H., Joshi S., Hew C.L., Leung K.Y. Comparative proteomic analysis of extracellular proteins of Edwardsiella tarda. Infect. Immun. 2002, 70:6475-6480.
78. Taylor S.W., Fahy E., Ghosh S.S. Global organellar proteomics. Trends Biotechnol. 2003, 21:82-88.
79. Tjalsma H., Bolhuis A., Jongbloed J.D., Bron S., van Dijl J.M. Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome. Microbiol. Mol. Biol. Rev. 2000, 64:515-547.
80. Tjalsma H., Antelmann H., Jongbloed J.D., Braun P.G., Darmon E., Dorenbos R., Dubois J.Y., Westers H., Zanen G., Quax W.J., Kuipers O.P., Bron S., Hecker M., van Dijl J.M. Proteomics of protein secretion by Bacillus subtilis: separating the " secrets" of the secretome. Microbiol. Mol. Biol. Rev. 2004, 68:207-233.
81. Trias J., Nikaido H. Outer membrane protein D2 catalyzes facilitated diffusion of carbapenems and penems through the outer membrane of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 1990, 34:52-57.
82. Voigt B., Schweder T., Sibbald M.J., Albrecht D., Ehrenreich A., Bernhardt J., Feesche J., Maurer K.H., Gottschalk G., van Dijl J.M., Hecker M. The extracellular proteome of Bacillus licheniformis grown in different media and under different nutrient starvation conditions. Proteomics 2006, 6:268-281.
83. Voigt B., Antelmann H., Albrecht D., Ehrenreich A., Maurer K.H., Evers S., Gottschalk G., van Dijl J.M., Schweder T., Hecker M. Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J. Mol. Microbiol. Biotechnol. 2009, 16:53-68.
84. Weber A., Kögl S.A., Jung K. Time-dependent proteome alterations under osmotic stress during aerobic and anaerobic growth in Escherichia coli. J. Bacteriol. 2006, 188:7165-7175.
85. Weiner J.H., Li L. Proteome of the Escherichia coli envelope and technological challenges in membrane proteome analysis. Biochim. Biophys. Acta 2008, 1778:1698-1713.
86. Wolf C., Hochgräfe F., Kusch H., Albrecht D., Hecker M., Engelmann S. Proteomic analysis of antioxidant strategies of Staphylococcus aureus: diverse responses to different oxidants. Proteomics 2008, 8:3139-3153.
87. Wu C.C., Yates J.R. The application of mass spectrometry to membrane proteomics. Nat. Biotechnol. 2003, 21:262-267.
88. Wu C.C., MacCoss M.J., Howell K.E., Yates J.R. A method for the comprehensive proteomic analysis of membrane proteins. Nat. Biotechnol. 2003, 21:532-538.
89. Xia X.X., Han M.J., Lee S.Y., Yoo J.S. Comparison of the extracellular proteomes of Escherichia coli B and K-12 strains during high cell density cultivation. Proteomics 2008, 8:2089-2103.
90. Xu Z., Lee S.Y. Display of polyhistidine peptides on the Escherichia coli cell surface by using outer membrane protein C as an anchoring motif. Appl. Environ. Microbiol. 1999, 65:5142-5147.
91. Xu C., Wang S., Ren H., Lin X., Wu L., Peng X. Proteomic analysis on the expression of outer membrane proteins of Vibrio alginolyticus at different sodium concentrations. Proteomics 2005, 5:3142-3152.
92. Yoon S.H., Han M.-J., Lee S.Y., Jeong K.J., Yoo J.S. Combined transcriptome and proteome analysis of Escherichia coli during high cell density culture. Biotechnol. Bioeng. 2003, 81:753-767.
93. Yoshino T., Matsunaga T. Development of efficient expression system for protein display on bacterial magnetic particles. Biochem. Biophys. Res. Commun. 2005, 338:1678-1681.
94. Yoshino T., Matsunaga T. Efficient and stable display of functional proteins on bacterial magnetic particles using mms13 as a novel anchor molecule. Appl. Environ. Microbiol. 2006, 72:465-471.
95. Zeng H., Guo G., Mao X.H., Tong W.D., Zou Q.M. Proteomic insights into Helicobacter pylori coccoid forms under oxidative stress. Curr. Microbiol. 2008, 57:281-286.
96. Zhang G., Brokx S., Weiner J.H. Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nat. Biotechnol. 2006, 24:100-104.
97. Zhang D.F., Jiang B., Xiang Z.M., Wang S.Y. Functional characterisation of altered outer membrane proteins for tetracycline resistance in Escherichia coli. Int. J. Antimicrob. Agents 2008, 32:315-319.
98. Zhou H., Ranish J.A., Watts J.D., Aebersold R. Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry. Nat. Biotechnol. 2002, 20:512-515.
99. B. Proteomics 2001, 1:480-493.